Reviewed, UniProtKB/Swiss-Prot Q3M9J6 (AMPA_ANAVT)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	Ava_2727

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000019878
Sites
	Active site	272	1	Potential
	Active site	347	1	Potential
	Metal binding	260	1	Manganese 2 By similarity
	Metal binding	265	1	Manganese 1 By similarity
	Metal binding	265	1	Manganese 2 By similarity
	Metal binding	284	1	Manganese 2 By similarity
	Metal binding	343	1	Manganese 1 By similarity
	Metal binding	345	1	Manganese 1 By similarity
	Metal binding	345	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3M9J6-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: C251B158A09EEEEF	FASTA	491	51,902
	10 20 30 40 50 60 MAIQLSDKPL LEWAGDTLAI ALFEDAVELT GELASLDEKF AGILKELIAE EEFTGKANST 70 80 90 100 110 120 VFTRVSANIP VRKIILVGLG KTEAFNIDTL RRAAAAVGKV GKKQKSKVIG LSFPLWNNDP 130 140 150 160 170 180 TASSQAIAEG VQLALYQDNR FKSDPDDKGS QVETVELLGF AGQEAAINRA NQIVSGVILA 190 200 210 220 230 240 RQLVAAPANS VTPITMAETA QQIAQDYGLQ IEILEQEDCE KLGMGAFLGV ALASDLPPKF 250 260 270 280 290 300 IHLTYKPEST PKRKLAIVGK GLTFDSGGLN IKGAGSGIET MKIDMGGAAA TLGAAKAIAQ 310 320 330 340 350 360 IKPNVEVHFI SAVTENMISG KAMHPGDILT ASNGKTIEVN NTDAEGRLTL ADALVYTDKL 370 380 390 400 410 420 GLDAIVDLAT LTGANVIALG DDIAGLYTPD DALAGQLEQA ASESGEKIWR MPLEEKYFEG 430 440 450 460 470 480 LKSGIADMKN TGPRPGGSIT AALFLKQFVK DTPWAHLDIA GPVWADKENG YNGPGATGYG 490 VRLLVDWVLS E

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA22340.1.
RefSeq	YP_323235.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3M9J6.
Protein family/group databases
MEROPS	M17.002.
Genome annotation databases
GeneID	3682088.
GenomeReviews	Gene locus Ava_2727 in contig CP000117_GR.
KEGG	ava:Ava_2727.
NMPDR	fig|240292.3.peg.1131.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3M9J6.
OMA	LGHHNSG.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_2727-MON.
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_ANAVT

Accession

Primary (citable) accession number: Q3M9J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)