Reviewed, UniProtKB/Swiss-Prot Q3M8X6 (ISPH_ANAVT)

Names and origin

Protein names

Recommended name:
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
    EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	Ava_2949

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity.
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity.
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the ispH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021084

Sequences

	Sequence		Length	Mass (Da)
	Q3M8X6-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 6DB8BD048950D28B	FASTA	402	45,425
	10 20 30 40 50 60 MDTKTFKRTL QHSENYNRKG FGHQAEVATQ LQSEYQSSLI QEIRDRNYTL QRGDVTIRLA 70 80 90 100 110 120 QAFGFCWGVE RAVAMAYETR KHFPTERIWI TNEIIHNPSV NQRMQEMQVG FIPVEAGNKD 130 140 150 160 170 180 FSVVGNNDVV ILPAFGASVQ EMQLLSEKGC KIVDTTCPWV SKVWNTVEKH KKGDHTSIIH 190 200 210 220 230 240 GKYKHEETIA TSSFAGKYLI VLNLKEAQYV ADYILHGRNR EEFLQKFAKA CSAGFDPDKD 250 260 270 280 290 300 LERVGIANQT TMLKGETEQI GKLFEHTMLQ KYGPVELNQH FQSFNTICDA TQERQDAMLE 310 320 330 340 350 360 LVQENLDLMI VIGGFNSSNT TQLQQISQER GLPSYHIDVV ERIKSVNSIE HRQLNGELVT 370 380 390 400 TENWLPAGKI VVGVTSGAST PDKVVEDVIE KIFALKATTA VV

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA22560.1.
RefSeq	YP_323455.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3M8X6.
Genome annotation databases
GeneID	3681319.
GenomeReviews	Gene locus Ava_2949 in contig CP000117_GR.
KEGG	ava:Ava_2949.
NMPDR	fig|240292.3.peg.1801.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3M8X6.
OMA	KNDICYA.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_2949-MON.
Family and domain databases
HAMAP	MF_00191. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. ispH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_ANAVT

Accession

Primary (citable) accession number: Q3M8X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2005
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)