Reviewed, UniProtKB/Swiss-Prot Q3M8T9 (LEU3_ANAVT)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:	leuB
Ordered Locus Names:	Ava_2987

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate By similarity.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	3-isopropylmalate dehydrogenase		PRO_0000250100
Regions
	Nucleotide binding	78 – 91	14	NAD By similarity
	Nucleotide binding	284 – 296	13	NAD By similarity
Sites
	Metal binding	226	1	Magnesium or manganese By similarity
	Metal binding	250	1	Magnesium or manganese By similarity
	Metal binding	254	1	Magnesium or manganese By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	108	1	Substrate By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	226	1	Substrate By similarity
	Site	143	1	Important for catalysis By similarity
	Site	194	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3M8T9-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: F95CB20C7827D793	FASTA	362	38,849
	10 20 30 40 50 60 MTQNYRITLL PGDGIGPEIM AVAVDVLKVV GQQFDIQFDF QEALIGGAAI DATGEPLPSA 70 80 90 100 110 120 TLDTCRQSDA VLLAAIGGYK WDSLPPHQRP EGGLLGLRAG LELFANLRPA QILPQLIDAS 130 140 150 160 170 180 TLKREVVEGV DIMVVRELTG GIYFGKPRGI FTTETGEKRG VNTMVYTESE IDRIGRIAFE 190 200 210 220 230 240 TARKRRGKLC SVDKANVLDV SQLWRDRITK LSQEYPDVEL SHLYVDNAAM QLVRAPKQFD 250 260 270 280 290 300 TIVTGNLFGD ILSDAAAMLT GSIGMLPSAS LGASGPGVFE PVHGSAPDIA GLDKANPLAQ 310 320 330 340 350 360 VLSAAMMLRY ALNQPQAADK IEQAVLQVLE QGDRTGDIMS VGMNLLGCRA MGDSLIKALE KS

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA22597.1.
RefSeq	YP_323492.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3681222.
GenomeReviews	Gene locus Ava_2987 in contig CP000117_GR.
KEGG	ava:Ava_2987.
NMPDR	fig|240292.3.peg.2045.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3M8T9.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_2987-MON.
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_ANAVT

Accession

Primary (citable) accession number: Q3M8T9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	October 25, 2005
Last modified:	November 4, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)