Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q3M8N2

- PFKA_ANAVT

UniProt

Q3M8N2 - PFKA_ANAVT

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Anabaena variabilis (strain ATCC 29413 / PCC 7937)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (25 Oct 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Subject to allosteric activation by ADP and other diphosphonucleosides, and inhibition by phosphoenolpyruvate.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121ATP; via amide nitrogenUniRule annotation
    Metal bindingi108 – 1081Magnesium; catalyticUniRule annotation
    Sitei109 – 1091Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
    Active sitei133 – 1331Proton acceptorUniRule annotation
    Binding sitei168 – 1681Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei229 – 2291SubstrateUniRule annotation
    Binding sitei272 – 2721Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi80 – 812ATPUniRule annotation
    Nucleotide bindingi107 – 1104ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAVAR240292:GCY3-3081-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:Ava_3044
    OrganismiAnabaena variabilis (strain ATCC 29413 / PCC 7937)
    Taxonomic identifieri240292 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeAnabaena
    ProteomesiUP000002533: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 357357ATP-dependent 6-phosphofructokinasePRO_1000120025Add
    BLAST

    Proteomic databases

    PRIDEiQ3M8N2.

    Interactioni

    Subunit structurei

    Homodimer or homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi240292.Ava_3044.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3M8N2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni131 – 1333Substrate bindingUniRule annotation
    Regioni175 – 1773Substrate bindingUniRule annotation
    Regioni278 – 2814Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000248869.
    KOiK00850.
    OMAiGLMHEDY.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_01976. Phosphofructokinase_III.
    InterProiIPR012003. ATP_PFK_prok.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3M8N2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKKIGILTS GGDCPGLNCV IRAVVSHATL TYDWEVFGIP YATQGLLERQ    50
    AIALNMHGWD LRGIDPLLNM GGTILGTINK GDTLAHVDEM LASYEALALD 100
    ALIVIGGDGS LGILHELASR GNWNLVAIPK TIDSDVALTE RAVGFDTAVN 150
    TIVDALNRLT FTAASHDRVM IVEVMGRSAG HLALHAGIAG GADVILIPEI 200
    SYTISGLCQY IAELRDRWQR KFAIVVVAEG AKLCLEDVQE NIASACVSPK 250
    CGRGQYIAEQ IARCSKNLID TRVSVLGHIQ RGGIPSALDR LTATVFGKTA 300
    VDLVAQGKFG QMVAWQNGEA IPVPIQDVVA QSPLHVNPQG SLVQSARCLG 350
    IYVGEKT 357
    Length:357
    Mass (Da):38,010
    Last modified:October 25, 2005 - v1
    Checksum:i35C2655211A929E1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000117 Genomic DNA. Translation: ABA22654.1.
    RefSeqiWP_011319785.1. NC_007413.1.
    YP_323549.1. NC_007413.1.

    Genome annotation databases

    EnsemblBacteriaiABA22654; ABA22654; Ava_3044.
    GeneIDi3681162.
    KEGGiava:Ava_3044.
    PATRICi35427055. VBIAnaVar43351_4069.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000117 Genomic DNA. Translation: ABA22654.1 .
    RefSeqi WP_011319785.1. NC_007413.1.
    YP_323549.1. NC_007413.1.

    3D structure databases

    ProteinModelPortali Q3M8N2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 240292.Ava_3044.

    Proteomic databases

    PRIDEi Q3M8N2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABA22654 ; ABA22654 ; Ava_3044 .
    GeneIDi 3681162.
    KEGGi ava:Ava_3044.
    PATRICi 35427055. VBIAnaVar43351_4069.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000248869.
    KOi K00850.
    OMAi GLMHEDY.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci AVAR240292:GCY3-3081-MONOMER.

    Family and domain databases

    HAMAPi MF_01976. Phosphofructokinase_III.
    InterProi IPR012003. ATP_PFK_prok.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Anabaena variabilis ATCC 29413."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29413 / PCC 7937.

    Entry informationi

    Entry nameiPFKA_ANAVT
    AccessioniPrimary (citable) accession number: Q3M8N2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: October 25, 2005
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3