Reviewed, UniProtKB/Swiss-Prot Q3M8N2 (K6PF_ANAVT)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase
      Short name=Phosphofructokinase
    EC=2.7.1.11
Alternative name(s):
    Phosphohexokinase

Gene names

Name:	pfkA
Ordered Locus Names:	Ava_3044

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity HAMAP MF_00339.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	6-phosphofructokinase HAMAP MF_00339		PRO_1000120025
Regions
	Nucleotide binding	22 – 26	5	ATP By similarity
	Nucleotide binding	160 – 164	5	ATP By similarity
	Nucleotide binding	177 – 193	17	ATP By similarity
Sites
	Active site	133	1	Proton acceptor By similarity
	Metal binding	191	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	193	1	Magnesium By similarity
	Binding site	168	1	Substrate By similarity
	Binding site	272	1	Substrate By similarity
	Binding site	278	1	Substrate By similarity
	Binding site	281	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3M8N2-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 35C2655211A929E1	FASTA	357	38,010
	10 20 30 40 50 60 MRKKIGILTS GGDCPGLNCV IRAVVSHATL TYDWEVFGIP YATQGLLERQ AIALNMHGWD 70 80 90 100 110 120 LRGIDPLLNM GGTILGTINK GDTLAHVDEM LASYEALALD ALIVIGGDGS LGILHELASR 130 140 150 160 170 180 GNWNLVAIPK TIDSDVALTE RAVGFDTAVN TIVDALNRLT FTAASHDRVM IVEVMGRSAG 190 200 210 220 230 240 HLALHAGIAG GADVILIPEI SYTISGLCQY IAELRDRWQR KFAIVVVAEG AKLCLEDVQE 250 260 270 280 290 300 NIASACVSPK CGRGQYIAEQ IARCSKNLID TRVSVLGHIQ RGGIPSALDR LTATVFGKTA 310 320 330 340 350 VDLVAQGKFG QMVAWQNGEA IPVPIQDVVA QSPLHVNPQG SLVQSARCLG IYVGEKT

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA22654.1.
RefSeq	YP_323549.1.
3D structure databases
HSSP	HSSP built from PDB template 1ZXX based on UniProtKB P80019.
SMR	Q3M8N2. Positions 3-351.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3M8N2.
Genome annotation databases
GeneID	3681162.
GenomeReviews	Gene locus Ava_3044 in contig CP000117_GR.
KEGG	ava:Ava_3044.
NMPDR	fig|240292.3.peg.2267.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HBG405044.
OMA	ASSHKRV.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_3044-MONOMER.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_ANAVT

Accession

Primary (citable) accession number: Q3M8N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 25, 2005
Last modified:	January 19, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)