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Q3M8N2 (PFKA_ANAVT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Ordered Locus Names:Ava_3044
OrganismAnabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]
Taxonomic identifier240292 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeAnabaena

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_01976

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_01976

Cofactor

Magnesium By similarity. HAMAP-Rule MF_01976

Enzyme regulation

Subject to allosteric activation by ADP and other diphosphonucleosides, and inhibition by phosphoenolpyruvate By similarity. HAMAP-Rule MF_01976

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_01976

Subunit structure

Homodimer or homotetramer By similarity. HAMAP-Rule MF_01976

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01976.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 357357ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_01976
PRO_1000120025

Regions

Nucleotide binding80 – 812ATP By similarity
Nucleotide binding107 – 1104ATP By similarity
Region131 – 1333Substrate binding By similarity
Region175 – 1773Substrate binding By similarity
Region278 – 2814Substrate binding By similarity

Sites

Active site1331Proton acceptor By similarity
Metal binding1081Magnesium; catalytic By similarity
Binding site121ATP; via amide nitrogen By similarity
Binding site1681Substrate; shared with dimeric partner By similarity
Binding site2291Substrate By similarity
Binding site2721Substrate; shared with dimeric partner By similarity
Site1091Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3M8N2 [UniParc].

Last modified October 25, 2005. Version 1.
Checksum: 35C2655211A929E1

FASTA35738,010
        10         20         30         40         50         60 
MRKKIGILTS GGDCPGLNCV IRAVVSHATL TYDWEVFGIP YATQGLLERQ AIALNMHGWD 

        70         80         90        100        110        120 
LRGIDPLLNM GGTILGTINK GDTLAHVDEM LASYEALALD ALIVIGGDGS LGILHELASR 

       130        140        150        160        170        180 
GNWNLVAIPK TIDSDVALTE RAVGFDTAVN TIVDALNRLT FTAASHDRVM IVEVMGRSAG 

       190        200        210        220        230        240 
HLALHAGIAG GADVILIPEI SYTISGLCQY IAELRDRWQR KFAIVVVAEG AKLCLEDVQE 

       250        260        270        280        290        300 
NIASACVSPK CGRGQYIAEQ IARCSKNLID TRVSVLGHIQ RGGIPSALDR LTATVFGKTA 

       310        320        330        340        350 
VDLVAQGKFG QMVAWQNGEA IPVPIQDVVA QSPLHVNPQG SLVQSARCLG IYVGEKT 

« Hide

References

[1]"Complete sequence of Anabaena variabilis ATCC 29413."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29413 / PCC 7937.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000117 Genomic DNA. Translation: ABA22654.1.
RefSeqYP_323549.1. NC_007413.1.

3D structure databases

ProteinModelPortalQ3M8N2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING240292.Ava_3044.

Proteomic databases

PRIDEQ3M8N2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA22654; ABA22654; Ava_3044.
GeneID3681162.
KEGGava:Ava_3044.
PATRIC35427055. VBIAnaVar43351_4069.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248869.
KOK00850.
OMAGLMHEDY.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

BioCycAVAR240292:GCY3-3081-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_01976. Phosphofructokinase_III.
InterProIPR012003. ATP_PFK_prok.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA_ANAVT
AccessionPrimary (citable) accession number: Q3M8N2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 25, 2005
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways