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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Anabaena variabilis (strain ATCC 29413 / PCC 7937)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Subject to allosteric activation by ADP and other diphosphonucleosides, and inhibition by phosphoenolpyruvate.By similarity

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12ATP; via amide nitrogenUniRule annotation1
Metal bindingi108Magnesium; catalyticUniRule annotation1
Sitei109Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation1
Active sitei133Proton acceptorUniRule annotation1
Binding sitei168Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei229SubstrateUniRule annotation1
Binding sitei272Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi80 – 81ATPUniRule annotation2
Nucleotide bindingi107 – 110ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAVAR240292:G7WH-7224-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Ava_3044
OrganismiAnabaena variabilis (strain ATCC 29413 / PCC 7937)
Taxonomic identifieri240292 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeAnabaena
Proteomesi
  • UP000002533 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001200251 – 357ATP-dependent 6-phosphofructokinaseAdd BLAST357

Proteomic databases

PRIDEiQ3M8N2.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi240292.Ava_3044.

Structurei

3D structure databases

ProteinModelPortaliQ3M8N2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 133Substrate bindingUniRule annotation3
Regioni175 – 177Substrate bindingUniRule annotation3
Regioni278 – 281Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R1X. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248869.
KOiK00850.
OMAiPEISYTI.
OrthoDBiPOG091H01AC.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3M8N2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKKIGILTS GGDCPGLNCV IRAVVSHATL TYDWEVFGIP YATQGLLERQ
60 70 80 90 100
AIALNMHGWD LRGIDPLLNM GGTILGTINK GDTLAHVDEM LASYEALALD
110 120 130 140 150
ALIVIGGDGS LGILHELASR GNWNLVAIPK TIDSDVALTE RAVGFDTAVN
160 170 180 190 200
TIVDALNRLT FTAASHDRVM IVEVMGRSAG HLALHAGIAG GADVILIPEI
210 220 230 240 250
SYTISGLCQY IAELRDRWQR KFAIVVVAEG AKLCLEDVQE NIASACVSPK
260 270 280 290 300
CGRGQYIAEQ IARCSKNLID TRVSVLGHIQ RGGIPSALDR LTATVFGKTA
310 320 330 340 350
VDLVAQGKFG QMVAWQNGEA IPVPIQDVVA QSPLHVNPQG SLVQSARCLG

IYVGEKT
Length:357
Mass (Da):38,010
Last modified:October 25, 2005 - v1
Checksum:i35C2655211A929E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000117 Genomic DNA. Translation: ABA22654.1.

Genome annotation databases

EnsemblBacteriaiABA22654; ABA22654; Ava_3044.
KEGGiava:Ava_3044.
PATRICi35427055. VBIAnaVar43351_4069.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000117 Genomic DNA. Translation: ABA22654.1.

3D structure databases

ProteinModelPortaliQ3M8N2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi240292.Ava_3044.

Proteomic databases

PRIDEiQ3M8N2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABA22654; ABA22654; Ava_3044.
KEGGiava:Ava_3044.
PATRICi35427055. VBIAnaVar43351_4069.

Phylogenomic databases

eggNOGiENOG4107R1X. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248869.
KOiK00850.
OMAiPEISYTI.
OrthoDBiPOG091H01AC.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciAVAR240292:G7WH-7224-MONOMER.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA_ANAVT
AccessioniPrimary (citable) accession number: Q3M8N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 25, 2005
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.