Q3M8C8 (SYK_ANAVT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysine--tRNA ligase
EC=6.1.1.6

Alternative name(s):
Lysyl-tRNA synthetase
Short name=LysRS

Gene names

Name:	lysS
Ordered Locus Names:	Ava_3150

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00252
Cofactor	Binds 3 magnesium ions per subunit By similarity. HAMAP MF_00252
Subunit structure	Homodimer By similarity. HAMAP MF_00252
Subcellular location	Cytoplasm HAMAP MF_00252.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW lysine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Lysine--tRNA ligase HAMAP MF_00252		PRO_1000012838
Sites
	Metal binding	409	1	Magnesium 1 By similarity
	Metal binding	416	1	Magnesium 1 By similarity
	Metal binding	416	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3M8C8 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: C934A42ECCFA51EE	FASTA	561	63,747
	10 20 30 40 50 60 MSEEDIRAAR LEKVEQLKQL GTNPYAYRWE STHHAAQLQE KFADLTSGEE VDTEVAIAGR 70 80 90 100 110 120 IMARRVFGKL AFFTLEDETG TIQLYLEKNR IQESMAETDA DAFNHLKQLT DVGDILGAKG 130 140 150 160 170 180 TIKRTEKGEL SIYVKEYTIL TKSLLPLPDK WHGLTDVAKR YRQRYVDLIV NPEVRQTFRR 190 200 210 220 230 240 RAQITAGIRR YLEQRDFLEI ETPVLQSETG GADARPFITY HNTLEMELYL RIATELHLKR 250 260 270 280 290 300 LIVGGFEKVF ELGRVFRNEG ISTRHNPEFT SIEIYQAYAD YNDMMALTEG IITTVAQDVL 310 320 330 340 350 360 GTLQITYQGE TVDLTPPWRR VTMHDLVKEY TGLDFHSFQT LEEAKSASKN AGIPGVDEAQ 370 380 390 400 410 420 TIGKILNLAF EEKVEANLIQ PTFVIDYPVE ISPLAKPHRS QPGLVERFEL FIVGRETANS 430 440 450 460 470 480 FSELTDPIDQ RERLEAQAAK KAAGDLEAQG VDEDFLTALE YGMPPTGGLG IGIDRLVMLL 490 500 510 520 530 540 TDSASIRDVI AFPLLKPEGS FIKEYRYEST TQTLTMEFDS GSVYEYFKVP LTVKEELDNA 550 560 PSKGQYFHKF IKGKFKYEQL S

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29413 / PCC 7937.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA22758.1.
RefSeq	YP_323653.1. NC_007413.1.
3D structure databases
ProteinModelPortal	Q3M8C8.
SMR	Q3M8C8. Positions 455-493.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3M8C8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3680784.
GenomeReviews	Gene locus Ava_3150 in contig CP000117_GR.
KEGG	ava:Ava_3150.
NMPDR	fig|240292.3.peg.3155.
PATRIC	35427321. VBIAnaVar43351_4197.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1190.
HOGENOM	HBG631383.
OMA	VEHKLEQ.
PhylomeDB	Q3M8C8.
ProtClustDB	PRK00484.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_3150-MONOMER.
Family and domain databases
HAMAP	MF_00252. Lys_tRNA_synth_class2. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR002313. Lys-tRNA-synth_II. IPR018149. Lys-tRNA-synth_II_C. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K04567.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF4. tRNA-synt_lys_2. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00499. LysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK_ANAVT

Accession

Primary (citable) accession number: Q3M8C8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2005
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program