ID   PNP_ANAVT               Reviewed;         718 AA.
AC   Q3M7Z9;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   16-JUN-2009, entry version 24.
DE   RecName: Full=Polyribonucleotide nucleotidyltransferase;
DE            EC=2.7.7.8;
DE   AltName: Full=Polynucleotide phosphorylase;
DE            Short=PNPase;
GN   Name=pnp; OrderedLocusNames=Ava_3279;
OS   Anabaena variabilis (strain ATCC 29413 / PCC 7937).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Anabaena.
OX   NCBI_TaxID=240292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J.,
RA   Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P.;
RT   "Complete sequence of Anabaena variabilis ATCC 29413.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in mRNA degradation. Hydrolyzes single-stranded
CC       polyribonucleotides processively in the 3'- to 5'-direction (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: RNA(n+1) + phosphate = RNA(n) + a nucleoside
CC       diphosphate.
CC   -!- SUBUNIT: Homotrimer. Organized into a structure (processome or RNA
CC       degradosome) containing a number of RNA-processing enzymes (By
CC       similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the polyribonucleotide
CC       nucleotidyltransferase family.
CC   -!- SIMILARITY: Contains 1 KH domain.
CC   -!- SIMILARITY: Contains 1 S1 motif domain.
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DR   EMBL; CP000117; ABA22887.1; -; Genomic_DNA.
DR   RefSeq; YP_323782.1; -.
DR   GeneID; 3680310; -.
DR   GenomeReviews; CP000117_GR; Ava_3279.
DR   KEGG; ava:Ava_3279; -.
DR   NMPDR; fig|240292.3.peg.2302; -.
DR   HOGENOM; Q3M7Z9; -.
DR   OMA; Q3M7Z9; GHGNLAK.
DR   BioCyc; AVAR240292:AVA_3279-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000175; F:3'-5'-exoribonuclease activity; IEA:InterPro.
DR   GO; GO:0004654; F:polyribonucleotide nucleotidyltransferase a...; IEA:HAMAP.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006402; P:mRNA catabolic process; IEA:HAMAP.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   HAMAP; MF_01595; -; 1.
DR   InterPro; IPR001247; ExoRNase_PH_dom1.
DR   InterPro; IPR015847; ExoRNase_PH_dom2.
DR   InterPro; IPR004087; KH.
DR   InterPro; IPR004088; KH_type_1.
DR   InterPro; IPR018111; KH_type_1_subgr.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR012162; PNPase.
DR   InterPro; IPR015848; PNPase_PH_RNA-bd_bac/org-type.
DR   InterPro; IPR003029; Rbsml_prot_S1_RNA-bd_dom.
DR   Gene3D; G3DSA:2.40.50.140; OB_NA_bd_sub; 1.
DR   Gene3D; G3DSA:1.10.10.400; PNPase_PH_RNA-bd_bac/org-type; 1.
DR   PANTHER; PTHR11252; PNPase; 1.
DR   Pfam; PF00013; KH_1; 1.
DR   Pfam; PF03726; PNPase; 1.
DR   Pfam; PF01138; RNase_PH; 2.
DR   Pfam; PF03725; RNase_PH_C; 2.
DR   Pfam; PF00575; S1; 1.
DR   PIRSF; PIRSF005499; PNPase; 1.
DR   SMART; SM00322; KH; 1.
DR   TIGRFAMs; TIGR03591; Polynuc_phos; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
DR   PROSITE; PS50126; S1; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Nucleotidyltransferase; RNA-binding;
KW   Transferase.
FT   CHAIN         1    718       Polyribonucleotide
FT                                nucleotidyltransferase.
FT                                /FTId=PRO_0000329497.
FT   DOMAIN      563    622       KH.
FT   DOMAIN      632    700       S1 motif.
SQ   SEQUENCE   718 AA;  77751 MW;  3526BD49C8984685 CRC64;
     MAEFEKSISF DGRDIRLKVG LLAPQAGGSV LIESGDTAVL VTATRSAGRE GIDFLPLTVD
     YEERLYAAGR IPGGIMRREG RPPEKTILTS RLIDRPLRPL FPSWLRDDLQ IVALTMSMDE
     QVPPDVLAVT GASIATLIAK IPFNGPMAAV RVGLVGDDFI INPTYAEIEA GDLDLVVAGS
     PHGVIMVEAG ANQLPERDII EAIDFGYEAV RDLIKAQLDL VAELGLEIVQ EAPPEVDQTL
     ENYIRDRASD EIKKILAQFE LTKPERDAAL DVVKDNIATA IAELPEEDPI RLAATANSKA
     LGNTFKDITK YFMRRQIVED NVRVDGRKLD QVRPVSSQVG VLPKRVHGSG LFNRGLTQVL
     SACTLGTPGD AQNLNDDLQT DQSKRYLHHY NFPPFSVGET KPLRAPGRRE IGHGALAERA
     ILPVLPPKEQ FPYVIRVVSE VLSSNGSTSM GSVCGSTLAL MDAGVPILKP VSGAAMGLIK
     EGDEVRVLTD IQGIEDFLGD MDFKVAGTDA GITALQMDMK ISGLSLEVIA QAIHQAKDAR
     LHILDKMLQT IDTPRTETSP YAPRLLTIKI DPDMIGLVIG PGGKTIKGIT EETGAKIDIE
     DDGTVTISAV DENKAKRARN IVQGMTRKLN EGDVYAGRVT RIIPIGAFVE FLPGKEGMIH
     ISQLADYRVG KVEDEVAVGD EVIVKVREID NKGRINLTRL GIHPDQAAAA REAAAVNR
//