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Q3M7Z9 (PNP_ANAVT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Polyribonucleotide nucleotidyltransferase
EC=2.7.7.8
Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase
Gene names
Name:pnp
Ordered Locus Names:Ava_3279
OrganismAnabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]
Taxonomic identifier240292 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length718 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP MF_01595

Catalytic activity

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595

Subunit structure

Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_01595.

Sequence similarities

Belongs to the polyribonucleotide nucleotidyltransferase family.

Contains 1 KH domain.

Contains 1 S1 motif domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandRNA-binding
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processRNA processing

Inferred from electronic annotation. Source: InterPro

mRNA catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function3'-5'-exoribonuclease activity

Inferred from electronic annotation. Source: InterPro

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

polyribonucleotide nucleotidyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 718718Polyribonucleotide nucleotidyltransferase HAMAP MF_01595
PRO_0000329497

Regions

Domain563 – 62260KH
Domain632 – 70069S1 motif

Sequences

Sequence LengthMass (Da)Tools
Q3M7Z9 [UniParc].

Last modified October 25, 2005. Version 1.
Checksum: 3526BD49C8984685

FASTA71877,751
        10         20         30         40         50         60 
MAEFEKSISF DGRDIRLKVG LLAPQAGGSV LIESGDTAVL VTATRSAGRE GIDFLPLTVD 

        70         80         90        100        110        120 
YEERLYAAGR IPGGIMRREG RPPEKTILTS RLIDRPLRPL FPSWLRDDLQ IVALTMSMDE 

       130        140        150        160        170        180 
QVPPDVLAVT GASIATLIAK IPFNGPMAAV RVGLVGDDFI INPTYAEIEA GDLDLVVAGS 

       190        200        210        220        230        240 
PHGVIMVEAG ANQLPERDII EAIDFGYEAV RDLIKAQLDL VAELGLEIVQ EAPPEVDQTL 

       250        260        270        280        290        300 
ENYIRDRASD EIKKILAQFE LTKPERDAAL DVVKDNIATA IAELPEEDPI RLAATANSKA 

       310        320        330        340        350        360 
LGNTFKDITK YFMRRQIVED NVRVDGRKLD QVRPVSSQVG VLPKRVHGSG LFNRGLTQVL 

       370        380        390        400        410        420 
SACTLGTPGD AQNLNDDLQT DQSKRYLHHY NFPPFSVGET KPLRAPGRRE IGHGALAERA 

       430        440        450        460        470        480 
ILPVLPPKEQ FPYVIRVVSE VLSSNGSTSM GSVCGSTLAL MDAGVPILKP VSGAAMGLIK 

       490        500        510        520        530        540 
EGDEVRVLTD IQGIEDFLGD MDFKVAGTDA GITALQMDMK ISGLSLEVIA QAIHQAKDAR 

       550        560        570        580        590        600 
LHILDKMLQT IDTPRTETSP YAPRLLTIKI DPDMIGLVIG PGGKTIKGIT EETGAKIDIE 

       610        620        630        640        650        660 
DDGTVTISAV DENKAKRARN IVQGMTRKLN EGDVYAGRVT RIIPIGAFVE FLPGKEGMIH 

       670        680        690        700        710 
ISQLADYRVG KVEDEVAVGD EVIVKVREID NKGRINLTRL GIHPDQAAAA REAAAVNR

« Hide

References

[1]"Complete sequence of Anabaena variabilis ATCC 29413."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29413 / PCC 7937.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000117 Genomic DNA. Translation: ABA22887.1.
RefSeqYP_323782.1. NC_007413.1.

3D structure databases

HSSPHSSP built from PDB template 1X4M based on UniProtKB Q91WJ8.
ProteinModelPortalQ3M7Z9.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ3M7Z9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3680310.
GenomeReviewsGene locus Ava_3279 in contig CP000117_GR.
KEGGava:Ava_3279.
NMPDRfig|240292.3.peg.2302.
PATRIC35427615. VBIAnaVar43351_4342.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1185.
HOGENOMHBG382411.
OMAYGETVVL.
PhylomeDBQ3M7Z9.
ProtClustDBPRK11824.

Enzyme and pathway databases

BioCycAVAR240292:AVA_3279-MONOMER.

Family and domain databases

HAMAPMF_01595. PNPase.
[Tree]
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH.
IPR004088. KH_type_1.
IPR018111. KH_type_1_subgr.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR012162. PNPase.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
KOK00962.
PANTHERPTHR11252. PNPase. 1 hit.
PfamPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFPIRSF005499. PNPase. 1 hit.
SMARTSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMSSF46915. 3_ExoRNase. 1 hit.
SSF55666. 3_ExoRNase. 2 hits.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF54211. Ribosomal_S5_D2-typ_fold. 2 hits.
TIGRFAMsTIGR03591. Polynuc_phos. 1 hit.
PROSITEPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePNP_ANAVT
AccessionPrimary (citable) accession number: Q3M7Z9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 25, 2005
Last modified: January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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