Reviewed, UniProtKB/Swiss-Prot Q3M7Z9 (PNP_ANAVT)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide nucleotidyltransferase
    EC=2.7.7.8
Alternative name(s):
    Polynucleotide phosphorylase
      Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	Ava_3279

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 718	718	Polyribonucleotide nucleotidyltransferase HAMAP MF_01595		PRO_0000329497
Regions
	Domain	563 – 622	60	KH
	Domain	632 – 700	69	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	Q3M7Z9-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 3526BD49C8984685	FASTA	718	77,751
	10 20 30 40 50 60 MAEFEKSISF DGRDIRLKVG LLAPQAGGSV LIESGDTAVL VTATRSAGRE GIDFLPLTVD 70 80 90 100 110 120 YEERLYAAGR IPGGIMRREG RPPEKTILTS RLIDRPLRPL FPSWLRDDLQ IVALTMSMDE 130 140 150 160 170 180 QVPPDVLAVT GASIATLIAK IPFNGPMAAV RVGLVGDDFI INPTYAEIEA GDLDLVVAGS 190 200 210 220 230 240 PHGVIMVEAG ANQLPERDII EAIDFGYEAV RDLIKAQLDL VAELGLEIVQ EAPPEVDQTL 250 260 270 280 290 300 ENYIRDRASD EIKKILAQFE LTKPERDAAL DVVKDNIATA IAELPEEDPI RLAATANSKA 310 320 330 340 350 360 LGNTFKDITK YFMRRQIVED NVRVDGRKLD QVRPVSSQVG VLPKRVHGSG LFNRGLTQVL 370 380 390 400 410 420 SACTLGTPGD AQNLNDDLQT DQSKRYLHHY NFPPFSVGET KPLRAPGRRE IGHGALAERA 430 440 450 460 470 480 ILPVLPPKEQ FPYVIRVVSE VLSSNGSTSM GSVCGSTLAL MDAGVPILKP VSGAAMGLIK 490 500 510 520 530 540 EGDEVRVLTD IQGIEDFLGD MDFKVAGTDA GITALQMDMK ISGLSLEVIA QAIHQAKDAR 550 560 570 580 590 600 LHILDKMLQT IDTPRTETSP YAPRLLTIKI DPDMIGLVIG PGGKTIKGIT EETGAKIDIE 610 620 630 640 650 660 DDGTVTISAV DENKAKRARN IVQGMTRKLN EGDVYAGRVT RIIPIGAFVE FLPGKEGMIH 670 680 690 700 710 ISQLADYRVG KVEDEVAVGD EVIVKVREID NKGRINLTRL GIHPDQAAAA REAAAVNR

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA22887.1.
RefSeq	YP_323782.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3680310.
GenomeReviews	Gene locus Ava_3279 in contig CP000117_GR.
KEGG	ava:Ava_3279.
NMPDR	fig|240292.3.peg.2302.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3M7Z9.
OMA	Q3M7Z9. GHGNLAK.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_3279-MON.
Family and domain databases
HAMAP	MF_01595. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
PANTHER	PTHR11252. PNPase. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03591. Polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_ANAVT

Accession

Primary (citable) accession number: Q3M7Z9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)