Reviewed, UniProtKB/Swiss-Prot Q3M7Y8 (TPIS_ANAVT)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase
      Short name=TIM
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	Ava_3290

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity. HAMAP MF_00147
Subcellular location	Cytoplasm Probable HAMAP MF_00147.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Triosephosphate isomerase HAMAP MF_00147		PRO_0000307423
Sites
	Active site	96	1	Electrophile By similarity
	Active site	165	1	Proton acceptor By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	11	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3M7Y8-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 10BDF34603CF661D	FASTA	241	26,577
	10 20 30 40 50 60 MRKIVIAGNW KMFKTQAESQ EFLKEFLPAL EETPQEREVL LCVPFTDLAI LSQSLHGSLV 70 80 90 100 110 120 QLGAQNVHWA ENGAYTGEIS GPMLTEIGVR YVIVGHSERR QFFGETDETV NLRLQAAQKY 130 140 150 160 170 180 GLTPILCVGE TKQQRDSGET ESLIVSQLDK DLINVDQTNL VIAYEPIWAI GTGDTCETTE 190 200 210 220 230 240 ANRVIGLIRS QLKNPDVPIQ YGGSVKPNNI DEIMAQPEID GVLVGGASLE AASFARIVNY Q

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA22898.1.
RefSeq	YP_323793.1.
3D structure databases
HSSP	HSSP built from PDB template 1B9B based on UniProtKB P36204.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3M7Y8.
Genome annotation databases
GeneID	3680321.
GenomeReviews	Gene locus Ava_3290 in contig CP000117_GR.
KEGG	ava:Ava_3290.
NMPDR	fig|240292.3.peg.2096.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0149.
HOGENOM	HBG708281.
OMA	DIRSVQT.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_3290-MONOMER.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_ANAVT

Accession

Primary (citable) accession number: Q3M7Y8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	October 25, 2005
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)