Q3M6Z4 (PURL_ANAVT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Ava_3636

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena › Anabaena variabilis

Protein attributes

Sequence length	777 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 777	777	Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420		PRO_0000236646
Regions
	Nucleotide binding	107 – 118	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3M6Z4 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: D21EB57A49164600	FASTA	777	83,320
	10 20 30 40 50 60 MTATSPAPFS PQEIAAEGIK PEEYAEIVRR LGRHPNKAEL GMFGVMWSEH CCYKNSRPLL 70 80 90 100 110 120 KQFPTTGPRI LVGPGENAGV VDLGEGLQLA FKIESHNHPS AVEPFQGAAT GVGGILRDIF 130 140 150 160 170 180 TMGARPIALL NSLRFGSLED PKTQRLFSGV VAGISHYGNC VGVPTVGGEV YFDPAYSGNP 190 200 210 220 230 240 LVNVMALGLM ETPEIVKSGA SGIGNPVLYV GSTTGRDGMG GASFASAELS DESIDDRPAV 250 260 270 280 290 300 QVGDPFLEKS LIEACLEAFK TGAVVAAQDM GAAGITCSTS EMAAKGGVGI ELDLDKIPVR 310 320 330 340 350 360 ETGMIPYEYL LSESQERMLF VAHKGREQEL IDIFHRWGLQ AVVAGTVIAE PIVRILFQGA 370 380 390 400 410 420 IAAEIPADAL AENTPLYERE LLAEPPEYAR QAWEWSSDSL PTCTTAGIEI QGNLQSWQEI 430 440 450 460 470 480 LLTLLNTPTI ASKNWVYRQY DHQVQNNTVF LPGGADAAVV RLRPLEGQGK ITNTLSGVAA 490 500 510 520 530 540 TVDCNPRYVY LDPYEGAKAV VAEAARNLSC VGAEPLAVTD NLNFGSPEKP IGYWQLSEAC 550 560 570 580 590 600 RGLAEGCREL ATPVTGGNVS LYNETLDPQG NPQPIYPTPV VGMVGLITDL TKICGQGWQT 610 620 630 640 650 660 PGDVIYLLGA SITTLGASEY LATIHDTVAG RPPRVDFDLE RRVQKVCREG IYADWVRSAH 670 680 690 700 710 720 DCAEGGLVVA LAESCLAGNL GAEIHLDASG SQLQRLDEVL FGEGGARILV SVASTQQENW 730 740 750 760 770 ESYLQEHLGQ NWQKLGIVGN TDADLAVLTT DNQSLIRVSI EEMNDRYQNA IARRLAL

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29413 / PCC 7937.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA23242.1.
RefSeq	YP_324137.1. NC_007413.1.
3D structure databases
ProteinModelPortal	Q3M6Z4.
ModBase	Search...
Protein-protein interaction databases
STRING	240292.Ava_3636.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABA23242; ABA23242; Ava_3636.
GeneID	3679270.
KEGG	ava:Ava_3636.
PATRIC	35428441. VBIAnaVar43351_4753.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HOG000238227.
KO	K01952.
OMA	WSEHCCY.
ProtClustDB	PRK01213.
Enzyme and pathway databases
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00420. PurL_2.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_ANAVT

Accession

Primary (citable) accession number: Q3M6Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 25, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program