Reviewed, UniProtKB/Swiss-Prot Q3M6R3 (SYC_ANAVT)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Ava_3718

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Cysteinyl-tRNA synthetase HAMAP MF_00041		PRO_0000240886
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	271 – 275	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	214	1	Zinc By similarity
	Metal binding	239	1	Zinc By similarity
	Metal binding	243	1	Zinc By similarity
	Binding site	274	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3M6R3-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 95C7EE7DFFA304C5	FASTA	486	55,152
	10 20 30 40 50 60 MTLTIYNTLT RRQEPLETVE PGKVKMYCCG VTVYDYCHLG HARSYIVWDT IRRYLIWRGF 70 80 90 100 110 120 EVKYIQNFTD IDDKILNRAK EQGSTMAEVS NRFIDAYFAD IRRLNVLDAD EYPRVTEHIP 130 140 150 160 170 180 EIHQLIQILE EKGLAYAVGG DVYYRVERFP SYGKLSGREL EQMQAGASGR VDVEDSEPKK 190 200 210 220 230 240 QHPFDFALWK AAKPGEPAWD SPWGQGRPGW HIECSAMIRS KLGATIDIHG GGGDLIFPHH 250 260 270 280 290 300 ENEIAQSEAA MNQPLARYWT HNGMVMVNGQ KMSKSLGNFI TIRELLDGVG SWKGDPVNPM 310 320 330 340 350 360 AVRLFVLQAH YRKPLDFTEE AIANAENSWK TLKEGLLFGY QYGEKLGWGQ ESAIIPELAT 370 380 390 400 410 420 RFQELGDDDF NFSGGLAVLF ELAKELRREG NILVHEGTTK TPSDELQRQW NTLVTLGKVL 430 440 450 460 470 480 GLEATPDDET LTQPGLSDTD IEALIEQRQA ARKNKNFSES DRIRNELQAQ GVTLIDSPQG TRWHRS

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA23323.1.
RefSeq	YP_324218.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3678922.
GenomeReviews	Gene locus Ava_3718 in contig CP000117_GR.
KEGG	ava:Ava_3718.
NMPDR	fig|240292.3.peg.4871.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3M6R3.
OMA	Q3M6R3. VLWKAAK.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_3718-MON.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ANAVT

Accession

Primary (citable) accession number: Q3M6R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)