Reviewed, UniProtKB/Swiss-Prot Q3M6L1 (RTCA_ANAVT)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	Ava_3770

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000264793
Sites
	Active site	316	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3M6L1-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: E789541292B3F3FA	FASTA	348	36,668
	10 20 30 40 50 60 MIDIDGSYGE GGGQVLRTSL SLAAITGEPI RIAGIRAGRR KPGLAAQHLT AVRAAARICH 70 80 90 100 110 120 GELQGDALGS TMLEFIPGGG VKAGNYIFDV SEVQQGGSAG AITLVLQTIL LPLALADGDS 130 140 150 160 170 180 HITLRGGTHV IFSPTVTYIE RVYLPMLCRM GIKAQVKLGA WGWYPRGGGE VNLQVKGGCQ 190 200 210 220 230 240 LCGLNLLERG ELKRVQGLAV ATELPAHIPQ RMANRAENLL RTAGLRVSMQ ALREKGVAPG 250 260 270 280 290 300 AGIFLTAEYC NSLTGFGGFG RLRLSSEKVA EIACGQLLQF HETGAPVDEH LADQLLLPAA 310 320 330 340 LASESSQYRV AEVSTHLTTN AAVIEKFGLG KITVNQAERV VAIASDKT

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA23375.1.
RefSeq	YP_324270.1.
3D structure databases
SMR	Q3M6L1. Positions 1-343.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3M6L1.
Genome annotation databases
GeneID	3678974.
GenomeReviews	Gene locus Ava_3770 in contig CP000117_GR.
KEGG	ava:Ava_3770.
NMPDR	fig|240292.3.peg.3888.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0430.
HOGENOM	HBG683539.
OMA	EIDGSYG.
PhylomeDB	Q3M6L1.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_3770-MONOMER.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR000228. RNA3'_term_phos_cycl-like. IPR013796. RNA3'_term_phos_cycl_insert. IPR020723. RNA3'_term_phos_cycl_prd. IPR017770. RNA3'_term_phos_cycl_sub. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_ANAVT

Accession

Primary (citable) accession number: Q3M6L1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	October 25, 2005
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)