Reviewed, UniProtKB/Swiss-Prot Q3M6H4 (PROB_ANAVT)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	Ava_3807

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Glutamate 5-kinase HAMAP MF_00456		PRO_0000230036
Regions
	Domain	277 – 355	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q3M6H4-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: FA118F16A3338F9D	FASTA	369	39,704
	10 20 30 40 50 60 MTKTIVVKIG TSSLTQPETG QLALSTIATL AETLSYLRQQ GNQVILVSSG AVGVGCARLG 70 80 90 100 110 120 LTERPKAIAL KQAVAAVGQG RLMRVYDDLF TTLQQPIAQV LLTRSDLVQR SRYLNVYNTF 130 140 150 160 170 180 RELLALGVIP VVNENDTVAV DELKFGDNDT LSALVASLVE ADWLFLLTDV DRLYSADPRS 190 200 210 220 230 240 VPDARPISLV TSIKELADLQ VQTGSQGSQW GTGGMMTKIS AARIAIAAGV RTVITQGRYP 250 260 270 280 290 300 RNIEKIIQGE LIGTHFQPQP EPTSARKRWI AYGLVPTGKL YLDSGAIAAI VKAGKSLLPA 310 320 330 340 350 360 GVKTVAGEFE PQDAVQLLDP QGNEIARGLV NYSSKDLQQI CGRHSKEIST ILGYVGAETV IHRDNLVLT

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA23412.1.
RefSeq	YP_324307.1.
3D structure databases
SMR	Q3M6H4. Positions 2-369.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3M6H4.
Genome annotation databases
GeneID	3678777.
GenomeReviews	Gene locus Ava_3807 in contig CP000117_GR.
KEGG	ava:Ava_3807.
NMPDR	fig|240292.3.peg.4050.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HBG507643.
OMA	TDVDRLY.
PhylomeDB	Q3M6H4.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_3807-MONOMER.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. IPR015947. PUA-like. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_ANAVT

Accession

Primary (citable) accession number: Q3M6H4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	October 25, 2005
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)