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Q3M4U9 (BIOB_ANAVT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Ava_4389
OrganismAnabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]
Taxonomic identifier240292 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeAnabaena

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335Biotin synthase HAMAP-Rule MF_01694
PRO_0000381204

Sites

Metal binding561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1001Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1321Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1921Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2641Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3M4U9 [UniParc].

Last modified October 25, 2005. Version 1.
Checksum: EFE32908EE151D97

FASTA33537,264
        10         20         30         40         50         60 
MSVGRIRYDW HTAEIRAVYD MPLLELIYQA ASVHRQFHNP KQIQVCKLIS IKTGGCPEDC 

        70         80         90        100        110        120 
SYCAQSSRYQ TEVKPQALLD KQTVVEIAQN AKQKGVSRVC MGAAWREVRD NSQFDRVLEM 

       130        140        150        160        170        180 
VKDVTDMGLE VCCTLGMLTT DQAKKLETAG LYAYNHNLDT SSDYYSTIIT TRTYSDRLNT 

       190        200        210        220        230        240 
IENVRQTNVT VCSGGILGLG ESIDDRVAML QTLATLNPHP ESVPINILSQ VEGTPLEDQP 

       250        260        270        280        290        300 
DVPVWDVVRM IATARIVMPT SDVRLSAGRA RLSQVEQAFC FMAGANSIFS SDDNKMLTVT 

       310        320        330 
TPCPDYDADQ EMLNLLGLEM RPPSQRPQPV VMGNS 

« Hide

References

[1]"Complete sequence of Anabaena variabilis ATCC 29413."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29413 / PCC 7937.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000117 Genomic DNA. Translation: ABA23987.1.
RefSeqYP_324882.1. NC_007413.1.

3D structure databases

ProteinModelPortalQ3M4U9.
SMRQ3M4U9. Positions 7-319.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING240292.Ava_4389.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA23987; ABA23987; Ava_4389.
GeneID3680575.
KEGGava:Ava_4389.
PATRIC35430183. VBIAnaVar43351_5614.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMACKEDCIF.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycAVAR240292:GCY3-4445-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_ANAVT
AccessionPrimary (citable) accession number: Q3M4U9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 25, 2005
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways