Reviewed, UniProtKB/Swiss-Prot Q3M4F6 (DXS_ANAVT)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	Ava_4532

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	635 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity. HAMAP MF_00315
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 635	635	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000256371

Sequences

	Sequence		Length	Mass (Da)
	Q3M4F6-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 41DD7664865CDB54	FASTA	635	69,052
	10 20 30 40 50 60 MHLSEITHPN QLHGLSIRQL QQIARQIRDK HLQTVAEFGG HLGPGLGVVE LTLGLYQTLD 70 80 90 100 110 120 LDRDKVIWDV GHQAYPHKLI TGRYSDFHTL RQKDGIAGYL KRGENKFDHF GAGHASTSIS 130 140 150 160 170 180 AALGMALARD MNGEKFKAVA VIGDGALTGG MALEAINHAG HLPKTNLLVV LNDNEMSISP 190 200 210 220 230 240 NVGAIPRYLN KMRLSPPVQF IKDNFEEQFK HIPFVGESLS PELGRIKEGM KRLAVPKVGA 250 260 270 280 290 300 VFEELGFTYM GPVDGHNLEE LIATFQQAHQ IAGPVLVHVA TIKGKGYELA EKDQVGYHAQ 310 320 330 340 350 360 TPFNLTTGKA IPSNKPKPPA YAKVFSHTLV KLAEQNPKII GITAAMATGT GLDKLQAKLP 370 380 390 400 410 420 NQYIDVGIAE QHAVTLAAGL ACEGMRPVAA IYSTFLQRAY DQIIHDVCIQ NLPVFFCLDR 430 440 450 460 470 480 AGIVGSDGPT HQGMYDIAYL RCIPNIVMMA PKDEAEMQRM VVTGIEYTTG PIAMRFPRGN 490 500 510 520 530 540 GYGVPLMEEG WEPLEIGKGE ILRNGDDVLI IGYGTMVYPS MQAAEILSEH GIEATVINAR 550 560 570 580 590 600 FVKPLDTELI VPLAQKIGRV VTLEEGCVMG GFGSAVAEAL LDADVVVPVK RIGIPDVLVE 610 620 630 HATPDESKAE LGLTSRQIAE RVLQAYFQKQ VSAVI

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA24130.1.
RefSeq	YP_325025.1.
3D structure databases
SMR	Q3M4F6. Positions 3-624.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3M4F6.
Genome annotation databases
GeneID	3680136.
GenomeReviews	Gene locus Ava_4532 in contig CP000117_GR.
KEGG	ava:Ava_4532.
NMPDR	fig|240292.3.peg.4092.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	LLNDNEM.
PhylomeDB	Q3M4F6.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_4532-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. PF00456. Transketolase_N. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_ANAVT

Accession

Primary (citable) accession number: Q3M4F6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 25, 2005
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)