Q3M473 (GLGB_ANAVT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	Ava_4616

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685		PRO_0000260636
Sites
	Active site	434	1	Nucleophile By similarity
	Active site	487	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3M473 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: FFDAFBDBC68D583E	FASTA	764	88,744
	10 20 30 40 50 60 MPMTTIAPEQ VNRIVWNQHH DPFEILGSHP IEQNGKNVWV VRAYLPNASA AWVVLPEQRQ 70 80 90 100 110 120 EYAMQTVHDP HFFECIIETS ELSNYQLKTK EGEHERVSYD PYAFRSPRLT DFDLHLFAEG 130 140 150 160 170 180 NHHRIYEKLG AHFTEVDGVT GVYFAVWAPN ARNVSVLGDF NLWDGRKHQM RKGATGVWEL 190 200 210 220 230 240 FIPEIGVGEH YKYEIKNFAG HIYEKSDPFG FQQEPRPKTA SIVSNLNSYN WSDENWLEQR 250 260 270 280 290 300 RHTDPLTQPI SVYEVHLGSW LHAASAEPAK LPNGETEPVV IASELNPGAR FLTYRELASR 310 320 330 340 350 360 LIPYVKELGY THIELLPIAE HPFDGSWGYQ VTGYYAPTSR FGTPEDFMYF VDQCHQNNIG 370 380 390 400 410 420 VIVDWVPGHF PKDGHGLAFF DGTHLYEHAD SRKGEHKEWG TLVFNYSRNE VRNFLVANAL 430 440 450 460 470 480 FWFDKYHIDG IRVDAVASML YLDYCRKEGE WLPNQYGGRE NLEAADFLRQ VNHLLFSYFP 490 500 510 520 530 540 GVLSIAEEST DWPMVSWPTY TGGLGFNLKW NMGWMHDMLD YFSMDPWFRQ FHQNNVTFSM 550 560 570 580 590 600 WYNHSENFML ALSHDEVVHG KSNIIGKMPG DKWQKLANVR CLFAYMFAHP GKKTMFMSME 610 620 630 640 650 660 FGQWSEWNVW ADLEWPLLQF EPHQQLKKFF TELNKLYRSE PALYTLDFAR EGFDWIDCSD 670 680 690 700 710 720 NRHSVVSFIR REKDTENFVV VVCNFTPQPH SHYRIGVPEK GFYTELFNSD ARQYGGSNMG 730 740 750 760 NLGGKWTDDW SMHSRPYSLD LCLPPLGVLI LKMDKEKTAK ASGS

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29413 / PCC 7937.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000117 Genomic DNA. Translation: ABA24213.1.
RefSeq	YP_325108.1. NC_007413.1.
3D structure databases
ProteinModelPortal	Q3M473.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3M473.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3679966.
GenomeReviews	Gene locus Ava_4616 in contig CP000117_GR.
KEGG	ava:Ava_4616.
NMPDR	fig|240292.3.peg.3432.
PATRIC	35430693. VBIAnaVar43351_5868.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	DGTCLYE.
PhylomeDB	Q3M473.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_4616-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_ANAVT

Accession

Primary (citable) accession number: Q3M473

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 25, 2005
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program