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Q3M3Y3

- MEND_ANAVT

UniProt

Q3M3Y3 - MEND_ANAVT

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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Anabaena variabilis (strain ATCC 29413 / PCC 7937)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation, Mn2+UniRule annotation
  • thiamine diphosphateUniRule annotationNote: Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciAVAR240292:GCY3-4767-MONOMER.
UniPathwayiUPA00995.
UPA01057; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:Ava_4706
OrganismiAnabaena variabilis (strain ATCC 29413 / PCC 7937)
Taxonomic identifieri240292 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeAnabaena
ProteomesiUP000002533: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5835832-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthasePRO_0000341700Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi240292.Ava_4706.

Structurei

3D structure databases

ProteinModelPortaliQ3M3Y3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1165.
HOGENOMiHOG000218359.
KOiK02551.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamiPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3M3Y3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPIAYKNINQ LWAYVFTETL KRLGLAYAVI CPGSRSTPLA VAFAQQAPDI
60 70 80 90 100
EGISILDERS AAFFALGLAK ATNRPVAIVC TSGTAGANFY PAVIEAQESR
110 120 130 140 150
VPLLLLTADR PPELRDCHSG QTIDQVKLFG SYPNWQTELA LPVSDMGMLG
160 170 180 190 200
YLRQTVIHSW YRMQAPTPGP VHLNIPFRDP LAPIPDGADL SYLLTKFHPE
210 220 230 240 250
EFFAGITDTT SLPDHSQLSI PPEWLKSQRG IIIAGVAQPQ QPQEYCRAIA
260 270 280 290 300
RLSQTLQWPV LAEGLSPIRN YADLNPYLIS TYDLILRNQQ LATRLAPDMV
310 320 330 340 350
IQIGDMPTSK ELRTWIDTHQ PRRWVIDPSD QNLDPLHGRT THLRIRVEEL
360 370 380 390 400
GCKGVEEDKS SVSEYLQLWC NAETKVRVNV DETLDKMEDL VECKAAWLLS
410 420 430 440 450
QILPPETPLF IANSMPVRDV EFFWKPNNLR VRSHFNRGAN GIDGTLSTAL
460 470 480 490 500
GIAHRHQSSV LITGDLALLH DTNGFLIRNK FVGHLTIILI NNNGGGIFEM
510 520 530 540 550
LPIAKFEPPF EEFFGTPQDI DFAQLCTTYN VQHELIHSWV HLQQRLNPLP
560 570 580
NTGIRVLELR TNRKIDAQWR RDNLSNFAAD NII
Length:583
Mass (Da):65,551
Last modified:July 1, 2008 - v2
Checksum:i8F1C11A039C39BD9
GO

Sequence cautioni

The sequence ABA24303.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000117 Genomic DNA. Translation: ABA24303.1. Different initiation.
RefSeqiYP_325198.1. NC_007413.1.

Genome annotation databases

EnsemblBacteriaiABA24303; ABA24303; Ava_4706.
GeneIDi3679725.
KEGGiava:Ava_4706.
PATRICi35430897. VBIAnaVar43351_5969.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000117 Genomic DNA. Translation: ABA24303.1 . Different initiation.
RefSeqi YP_325198.1. NC_007413.1.

3D structure databases

ProteinModelPortali Q3M3Y3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 240292.Ava_4706.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABA24303 ; ABA24303 ; Ava_4706 .
GeneIDi 3679725.
KEGGi ava:Ava_4706.
PATRICi 35430897. VBIAnaVar43351_5969.

Phylogenomic databases

eggNOGi COG1165.
HOGENOMi HOG000218359.
KOi K02551.
OrthoDBi EOG6NWBQW.

Enzyme and pathway databases

UniPathwayi UPA00995 .
UPA01057 ; UER00164 .
BioCyci AVAR240292:GCY3-4767-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
HAMAPi MF_01659. MenD.
InterProi IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view ]
Pfami PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004983. MenD. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00173. menD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Anabaena variabilis ATCC 29413."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29413 / PCC 7937.

Entry informationi

Entry nameiMEND_ANAVT
AccessioniPrimary (citable) accession number: Q3M3Y3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: November 26, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3