Reviewed, UniProtKB/Swiss-Prot Q3M3Q5 (TRMB_ANAVT)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
Alternative name(s):
    tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	Ava_4784

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057		PRO_0000229153
Sites
	Binding site	43	1	S-adenosyl-L-methionine By similarity
	Binding site	68	1	S-adenosyl-L-methionine By similarity
	Binding site	95	1	S-adenosyl-L-methionine By similarity
	Binding site	121	1	S-adenosyl-L-methionine By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	157	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3M3Q5-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: EA13CC417B1D94D9	FASTA	215	24,541
	10 20 30 40 50 60 MSALPFVRVR QHVNPLAQKY LTPANPLEWE KVYSSPHQPL HLDIGCARGR FVLQMAQVEP 70 80 90 100 110 120 RWNFLGLEIR EPLVIEANQF RSQLGLSNLH YLYCNANNSL QPLLSSLPTG ILQRVTIQFP 130 140 150 160 170 180 DPWFKTRHAK RRVVQPELVQ DIANYLAVGG VVFLQSDMEF VAVEMCDRFA ANPAFKKVGS 190 200 210 GEWLSENPLP VATERETTTQ NRGEPVYRAL FERIS

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA24381.1.
RefSeq	YP_325276.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3679437.
GenomeReviews	Gene locus Ava_4784 in contig CP000117_GR.
KEGG	ava:Ava_4784.
NMPDR	fig|240292.3.peg.3610.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3M3Q5.
OMA	Q3M3Q5. KRRVVQP.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_4784-MON.
Family and domain databases
HAMAP	MF_01057. [Tree]
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
PANTHER	PTHR23417:SF1. Methyltransf_4. 1 hit.
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. CHP91. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_ANAVT

Accession

Primary (citable) accession number: Q3M3Q5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)