Reviewed, UniProtKB/Swiss-Prot Q3M3M2 (GLPK_ANAVT)

Names and origin

Protein names

Recommended name:
    Glycerol kinase
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase
    Glycerokinase
      Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Ava_4817

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism By similarity.
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Glycerol kinase HAMAP MF_00186		PRO_1000077408
Regions
	Nucleotide binding	414 – 418	5	ATP By similarity
Sites
	Binding site	16	1	Substrate By similarity
	Binding site	20	1	ATP By similarity
	Binding site	86	1	Substrate By similarity
	Binding site	138	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	265	1	ATP By similarity
	Binding site	313	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3M3M2-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 6F636D675FE8F34F	FASTA	500	55,073
	10 20 30 40 50 60 MQTSSSGYIL ALDLGTTGNR AFIFNNAGKI VAQAYKELTQ HYPQPGWLEH DAEEIWQDTC 70 80 90 100 110 120 WVMKTAIANA QISPSEIAAI GLTVQRETCL LWDKTTGKPL HKAIVWQDRR TAPLCHQLQE 130 140 150 160 170 180 KGYAQEIYSR TGLVVDAYFS ATKLRWLLDY ITGVDLKNVL AGTIDTWILW KLTGGKVHAT 190 200 210 220 230 240 DHSNASRTML MNLKTGEWDE QLLEILQIPA HILPQIQPSL GKFGLTDTSL LDTAIPITAI 250 260 270 280 290 300 LGDQQAALFG HGCDRPGLMK CTYGTGSFLV AHTGSNIVRS QHQLISTIAW TQYNSQENIN 310 320 330 340 350 360 IGYALEGSMF TSGACIQWLR DGIKLIKTAA ETETMANQVD DNGGVYFVPA FSGLGAPYWD 370 380 390 400 410 420 MNARGAFFGI TASVQPQHLV RAVLEAIAYQ VLEVVQAINA SCSSPMQRLI VDGGACENNF 430 440 450 460 470 480 LMQFQADVLG IPVERPTMRD TTVQGAAFAA GLAVGFWDSY TALVNQRQID RIFEPGEGSQ 490 500 NAIYNFATWQ KAVKRSLDWV

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA24414.1.
RefSeq	YP_325309.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3M3M2.
Genome annotation databases
GeneID	3679393.
GenomeReviews	Gene locus Ava_4817 in contig CP000117_GR.
KEGG	ava:Ava_4817.
NMPDR	fig|240292.3.peg.3672.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3M3M2.
OMA	SRCIIFD.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_4817-MON.
Family and domain databases
HAMAP	MF_00186. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. False negative. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_ANAVT

Accession

Primary (citable) accession number: Q3M3M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 25, 2005
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)