Reviewed, UniProtKB/Swiss-Prot Q3M3F2 (ISPE_ANAVT)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Ava_4887

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity.
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235058
Regions
	Nucleotide binding	99 – 109	11	ATP Potential
Sites
	Active site	11	1	By similarity
	Active site	141	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3M3F2-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 438D018C949773D0	FASTA	317	34,517
	10 20 30 40 50 60 MRSYKLIAPA KINLYLEIIG DRPDGYHELV MILQSIDLAD EIEIHSLSSE TIRVHCNHPQ 70 80 90 100 110 120 VPTDKSNLVY RAAELMATKF PEAFAKYGGV DITVHKHIPV AAGLAGGSTN AAAVLVGIDL 130 140 150 160 170 180 LWNLGLTQTE LEELGSILGS DVPFCVAGGT VIATGRGEQL SPLPNLDHIY IVLGKYRSLE 190 200 210 220 230 240 VSTAWAYKTY RQEYGSTYLR DTNDLASRAA AVHSGSIVKA IVEKDAVAIA QKLHNDLEKV 250 260 270 280 290 300 VLPSYPQVLQ LRELFASQPS VIGTMMSGSG PSVFALCENQ AQAEQVQQQV RQTIPDEDLE 310 LFVTRTITHG IQVLGNE

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA24484.1.
RefSeq	YP_325379.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3M3F2.
Genome annotation databases
GeneID	3679211.
GenomeReviews	Gene locus Ava_4887 in contig CP000117_GR.
KEGG	ava:Ava_4887.
NMPDR	fig|240292.3.peg.3792.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3M3F2.
OMA	VFYPIPL.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_4887-MON.
Family and domain databases
HAMAP	MF_00061. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_ANAVT

Accession

Primary (citable) accession number: Q3M3F2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	October 25, 2005
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)