Reviewed, UniProtKB/Swiss-Prot Q3M3A3 (SYL_ANAVT)

Names and origin

Protein names

Recommended name:
    Leucyl-tRNA synthetase
    EC=6.1.1.4
Alternative name(s):
    Leucine--tRNA ligase
      Short name=LeuRS

Gene names

Name:	leuS
Ordered Locus Names:	Ava_4936

Organism

Anabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]

Taxonomic identifier

240292 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Anabaena

Protein attributes

Sequence length	872 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP MF_00049
Subcellular location	Cytoplasm. HAMAP MF_00049
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 872	872	Leucyl-tRNA synthetase HAMAP MF_00049		PRO_1000009289
Regions
	Motif	42 – 52	11	"HIGH" region HAMAP MF_00049
	Motif	634 – 638	5	"KMSKS" region HAMAP MF_00049
Sites
	Binding site	637	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3M3A3-1 [UniParc]. Last modified October 25, 2005. Version 1. Checksum: 20AB279E2EC4045C	FASTA	872	98,513
	10 20 30 40 50 60 MDSRYNPAIL EEKWQKTWVE LGLDKTQTQS NKPKFYALSM FPYPSGSLHM GHVRNYTITD 70 80 90 100 110 120 VIARLKRMQG YRVLHPMGWD AFGLPAENAA IDRGVPPANW TYQNITQMRQ QLQRLGLSID 130 140 150 160 170 180 WDSEVATCSP DYYKWTQWIF LQFLQAGLAY QKEAAVNWDP IDQTVLANEQ VDNEGRSWRS 190 200 210 220 230 240 GAIVERKLLR QWFLKITDYA EELLNDLDKL TGWPERVKLM QANWIGKSVG AYLEFPIVGS 250 260 270 280 290 300 TEKIAVYTTR PDTVYGVSYV VLAPEHPLTK QVTSKTQQAV VDTFIQEVTN QSELERTAED 310 320 330 340 350 360 KPKRGVATGG KAINPFTGEE VPIWIADYVL YEYGTGAVMG VPAHDVRDFK FAQRYDLPID 370 380 390 400 410 420 FVIAAPDDVA GFDLSPTSET EEVTQVVQIE YNQAYTEPGI LINSGAFTGM TSTDAKQAIV 430 440 450 460 470 480 KYATEKGFGK ERIQYRLRDW LISRQRYWGA PIPVIHCPNC GIVPVPDKDL PVILPEEVEF 490 500 510 520 530 540 TGRGGSPLAQ LESWVNVPCP TCGSPAKRET DTMDTFIDSS WYFLRFTDAR NEAQVFESAK 550 560 570 580 590 600 TNDWMPVDQY VGGIEHAILH LLYSRFFTKV LRDRGLLNFD EPFERLLTQG MVQGLTYFNP 610 620 630 640 650 660 NKGGKDKWVP SHLVNPNDPR DPQTGEPLQR LYATMSKSKG NGVAPEDVIA KYGVDTARMF 670 680 690 700 710 720 ILFKAPPEKD LEWDEADVEG QFRFLNRVWR LVTDYVASGV NPKNKSGELS KSEKDLRRAI 730 740 750 760 770 780 HSAIQSVTED LEDEYQFNTA ISELMKLSNA LTDANGKDSR VYAEGIHTLV VLLAPFAPHI 790 800 810 820 830 840 AEELWQLLGN SESVHTQTWP AFDPAALVAD EITLVIQVNG KKRADIQVPS QADKAELEKY 850 860 870 ARESEVVQRH LEGKEIKKVI VVPGKLVNFV VG

References

[1]

"Complete sequence of Anabaena variabilis ATCC 29413." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000117 Genomic DNA. Translation: ABA24533.1.
RefSeq	YP_325428.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3679123.
GenomeReviews	Gene locus Ava_4936 in contig CP000117_GR.
KEGG	ava:Ava_4936.
NMPDR	fig|240292.3.peg.3656.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3M3A3.
OMA	Q3M3A3. MMFASPP.
Enzyme and pathway databases
BioCyc	AVAR240292:AVA_4936-MON.
Family and domain databases
HAMAP	MF_00049. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-synth_Ia_bac/mito. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF7. Leu_tRNAsyn_1a. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL_ANAVT

Accession

Primary (citable) accession number: Q3M3A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 25, 2005
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)