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Q3M3A3 (SYL_ANAVT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Ava_4936
OrganismAnabaena variabilis (strain ATCC 29413 / PCC 7937) [Complete proteome] [HAMAP]
Taxonomic identifier240292 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeAnabaena

Protein attributes

Sequence length872 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 872872Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009289

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif634 – 6385"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6371ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3M3A3 [UniParc].

Last modified October 25, 2005. Version 1.
Checksum: 20AB279E2EC4045C

FASTA87298,513
        10         20         30         40         50         60 
MDSRYNPAIL EEKWQKTWVE LGLDKTQTQS NKPKFYALSM FPYPSGSLHM GHVRNYTITD 

        70         80         90        100        110        120 
VIARLKRMQG YRVLHPMGWD AFGLPAENAA IDRGVPPANW TYQNITQMRQ QLQRLGLSID 

       130        140        150        160        170        180 
WDSEVATCSP DYYKWTQWIF LQFLQAGLAY QKEAAVNWDP IDQTVLANEQ VDNEGRSWRS 

       190        200        210        220        230        240 
GAIVERKLLR QWFLKITDYA EELLNDLDKL TGWPERVKLM QANWIGKSVG AYLEFPIVGS 

       250        260        270        280        290        300 
TEKIAVYTTR PDTVYGVSYV VLAPEHPLTK QVTSKTQQAV VDTFIQEVTN QSELERTAED 

       310        320        330        340        350        360 
KPKRGVATGG KAINPFTGEE VPIWIADYVL YEYGTGAVMG VPAHDVRDFK FAQRYDLPID 

       370        380        390        400        410        420 
FVIAAPDDVA GFDLSPTSET EEVTQVVQIE YNQAYTEPGI LINSGAFTGM TSTDAKQAIV 

       430        440        450        460        470        480 
KYATEKGFGK ERIQYRLRDW LISRQRYWGA PIPVIHCPNC GIVPVPDKDL PVILPEEVEF 

       490        500        510        520        530        540 
TGRGGSPLAQ LESWVNVPCP TCGSPAKRET DTMDTFIDSS WYFLRFTDAR NEAQVFESAK 

       550        560        570        580        590        600 
TNDWMPVDQY VGGIEHAILH LLYSRFFTKV LRDRGLLNFD EPFERLLTQG MVQGLTYFNP 

       610        620        630        640        650        660 
NKGGKDKWVP SHLVNPNDPR DPQTGEPLQR LYATMSKSKG NGVAPEDVIA KYGVDTARMF 

       670        680        690        700        710        720 
ILFKAPPEKD LEWDEADVEG QFRFLNRVWR LVTDYVASGV NPKNKSGELS KSEKDLRRAI 

       730        740        750        760        770        780 
HSAIQSVTED LEDEYQFNTA ISELMKLSNA LTDANGKDSR VYAEGIHTLV VLLAPFAPHI 

       790        800        810        820        830        840 
AEELWQLLGN SESVHTQTWP AFDPAALVAD EITLVIQVNG KKRADIQVPS QADKAELEKY 

       850        860        870 
ARESEVVQRH LEGKEIKKVI VVPGKLVNFV VG 

« Hide

References

[1]"Complete sequence of Anabaena variabilis ATCC 29413."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyrpides N., Mavrommatis K., Richardson P.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29413 / PCC 7937.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000117 Genomic DNA. Translation: ABA24533.1.
RefSeqYP_325428.1. NC_007413.1.

3D structure databases

ProteinModelPortalQ3M3A3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING240292.Ava_4936.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABA24533; ABA24533; Ava_4936.
GeneID3679123.
KEGGava:Ava_4936.
PATRIC35431409. VBIAnaVar43351_6223.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAKGNPLES.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycAVAR240292:GCY3-4999-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ANAVT
AccessionPrimary (citable) accession number: Q3M3A3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 25, 2005
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries