Q3LIE5 (ADPRM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Manganese-dependent ADP-ribose/CDP-alcohol diphosphatase
EC=3.6.1.13
EC=3.6.1.16
EC=3.6.1.53

Alternative name(s):
ADPRibase-Mn
CDP-choline phosphohydrolase

Gene names

Name:	ADPRM
Synonyms:	C17orf48
ORF Names:	MDS006, Nbla03831

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes ADP-ribose, IDP-ribose, CDP-glycerol, CDP-choline and CDP-ethanolamine, but not other non-reducing ADP-sugars or CDP-glucose. May be involved in immune cell signaling as suggested by the second-messenger role of ADP-ribose, which activates TRPM2 as a mediator of oxidative/nitrosative stress By similarity.
Catalytic activity	CDP-choline + H2O = CMP + phosphocholine. ADP-D-ribose + H2O = AMP + D-ribose 5-phosphate. CDP-glycerol + H2O = CMP + sn-glycerol 3-phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the ADPRibase-Mn family.
Sequence caution	The sequence AAF87317.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ADP-ribose diphosphatase activity Inferred from electronic annotation. Source: EC CDP-glycerol diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q3LIE5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q3LIE5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     202-205: LSEP → ELFL
     206-342: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Manganese-dependent ADP-ribose/CDP-alcohol diphosphatase		PRO_0000286567
Sites
	Metal binding	25	1	Zinc 1 By similarity
	Metal binding	27	1	Zinc 1 By similarity
	Metal binding	74	1	Zinc 1 By similarity
	Metal binding	74	1	Zinc 2 By similarity
	Metal binding	110	1	Zinc 2 By similarity
	Metal binding	241	1	Zinc 2 By similarity
	Metal binding	278	1	Zinc 2 By similarity
	Metal binding	280	1	Zinc 1 By similarity
Natural variations
	Alternative sequence	202 – 205	4	LSEP → ELFL in isoform 2.		VSP_025092
	Alternative sequence	206 – 342	137	Missing in isoform 2.		VSP_025093
	Natural variant	92	1	L → R. Corresponds to variant rs34940296 [ dbSNP | Ensembl ].		VAR_032125
	Natural variant	337	1	E → G. Corresponds to variant rs406446 [ dbSNP | Ensembl ].		VAR_032126
Experimental info
	Sequence conflict	113	1	F → S in BAF85318. Ref.4
	Sequence conflict	202	1	L → F in AAF87317. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 2DBD4499D9DA2AC2	FASTA	342	39,529
	10 20 30 40 50 60 MDDKPNPEAL SDSSERLFSF GVIADVQFAD LEDGFNFQGT RRRYYRHSLL HLQGAIEDWN 70 80 90 100 110 120 NESSMPCCVL QLGDIIDGYN AQYNASKKSL ELVMDMFKRL KVPVHHTWGN HEFYNFSREY 130 140 150 160 170 180 LTHSKLNTKF LEDQIVHHPE TMPSEDYYAY HFVPFPKFRF ILLDAYDLSV LGVDQSSPKY 190 200 210 220 230 240 EQCMKILREH NPNTELNSPQ GLSEPQFVQF NGGFSQEQLN WLNEVLTFSD TNQEKVVIVS 250 260 270 280 290 300 HLPIYPDASD NVCLAWNYRD ALAVIWSHEC VVCFFAGHTH DGGYSEDPFG VYHVNLEGVI 310 320 330 340 ETAPDSQAFG TVHVYPDKMM LKGRGRVPDR IMNYKKERAF HC
	Isoform 2 [UniParc]. Checksum: C830ED5DC8F1C833	FASTA	205	24,010
	10 20 30 40 50 60 MDDKPNPEAL SDSSERLFSF GVIADVQFAD LEDGFNFQGT RRRYYRHSLL HLQGAIEDWN 70 80 90 100 110 120 NESSMPCCVL QLGDIIDGYN AQYNASKKSL ELVMDMFKRL KVPVHHTWGN HEFYNFSREY 130 140 150 160 170 180 LTHSKLNTKF LEDQIVHHPE TMPSEDYYAY HFVPFPKFRF ILLDAYDLSV LGVDQSSPKY 190 200 EQCMKILREH NPNTELNSPQ GELFL

References

[1]	"Neuroblastoma oligo-capping cDNA project: toward the understanding of the genesis and biology of neuroblastoma." Ohira M., Morohashi A., Nakamura Y., Isogai E., Furuya K., Hamano S., Machida T., Aoyama M., Fukumura M., Miyazaki K., Suzuki Y., Sugano S., Hirato J., Nakagawara A. Cancer Lett. 197:63-68(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Neuroblastoma.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Thymus.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Placenta and Testis.
[5]	"Novel genes expressed in hematopoietic stem/progenitor cells from myelodysplastic syndrome patients." Huang C., Zhang C., Tu Y., Gu W., Wang Y., Han Z., Chen Z., Zhou J., Gu J., Huang Q., Yu Y., Xu S., Ren S., Fu G. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-217 (ISOFORM 1). Tissue: Hematopoietic stem cell.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB073393 mRNA. Translation: BAE45723.1. AK292629 mRNA. Translation: BAF85318.1. CH471108 Genomic DNA. Translation: EAW89995.1. CH471108 Genomic DNA. Translation: EAW89996.1. BC001294 mRNA. Translation: AAH01294.1. BC070155 mRNA. Translation: AAH70155.1. AF168715 mRNA. Translation: AAF87317.1. Frameshift.
IPI	IPI00025025. IPI00744968.
RefSeq	NP_064618.3. NM_020233.4.
UniGene	Hs.47668.
3D structure databases
ProteinModelPortal	Q3LIE5.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000369099.
PTM databases
PhosphoSite	Q3LIE5.
Polymorphism databases
DMDM	121942723.
Proteomic databases
PaxDb	Q3LIE5.
PRIDE	Q3LIE5.
Protocols and materials databases
DNASU	56985.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000379774; ENSP00000369099; ENSG00000170222. ENST00000468843; ENSP00000431622; ENSG00000170222. ENST00000527582; ENSP00000463134; ENSG00000170222.
GeneID	56985.
KEGG	hsa:56985.
UCSC	uc002gmt.3. human.
Organism-specific databases
CTD	56985.
GeneCards	GC17P010600.
HGNC	HGNC:30925. ADPRM.
HPA	HPA023265. HPA023820.
neXtProt	NX_Q3LIE5.
PharmGKB	PA142672231.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1409.
HOGENOM	HOG000154875.
HOVERGEN	HBG100432.
InParanoid	Q3LIE5.
KO	K01517.
OMA	LAWNYRD.
OrthoDB	EOG4TXBSP.
Gene expression databases
Bgee	Q3LIE5.
CleanEx	HS_C17orf48.
Genevestigator	Q3LIE5.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	56985.
NextBio	62667.

Entry information

Entry name

ADPRM_HUMAN

Accession

Primary (citable) accession number: Q3LIE5
Secondary accession number(s): A8K9B4 , D3DTS4, Q9BVD4, Q9NRU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.