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Protein

Epstein-Barr nuclear antigen 2

Gene

EBNA2

Organism
Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays a key role in the activation of the host resting B-cell and stimulation of B-cell proliferation. Acts by up-regulating the expression of viral EBNA1-6, LMP1, LMP2A and LMP2B genes, as well as several host genes including CD21, CD23 and MYC. Activates transcription by acting as an adapter molecule that binds to cellular sequence-specific DNA-binding proteins such as host CBF1, SMARCB1 and SPI1. Once EBNA2 is near promoter sites, its acidic activating domain recruits basal and activation-associated transcription factors TFIIB, TAF40, TFIIH components ERCC2 and ERCC3, and CBP in order to promote transcription. Alternatively, EBNA2 can affect activities of cell cycle regulators and retard cell cycle progression at G2/M phase. It also induces chromosomal instability, by disrupting mitotic checkpoints, multi-nucleation and formation of micronuclei in infected cells (By similarity).By similarity

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processHost-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Modulation of host cell cycle by virus, Modulation of host PP1 activity by virus, Transcription, Transcription regulation, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Epstein-Barr nuclear antigen 2
Short name:
EBNA-2
Short name:
EBV nuclear antigen 2
Gene namesi
Name:EBNA2
ORF Names:BYRF1
OrganismiEpstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10376 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000007641 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002266001 – 451Epstein-Barr nuclear antigen 2Add BLAST451

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with human SMARCB1/INI1, presumably generating an open chromatin conformation at the EBNA2-responsive target genes. Interacts with human WAPL. Interacts with host CBF1; this interaction allows transcriptional activation by EBNA2. Interacts with host general transcription factors GTF2B, ERCC2 and ERCC3. Interacts (via PXLXP motif) with host ZMYND11/BS69 (via MYND-type zinc finger) (By similarity).By similarity

Protein-protein interaction databases

IntActiQ3KSV2. 13 interactors.

Structurei

3D structure databases

SMRiQ3KSV2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati311 – 31212
Repeati313 – 31422
Repeati315 – 31632
Repeati317 – 31842
Repeati319 – 32052
Repeati321 – 32262
Repeati323 – 32462

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 176SMARCB1/INI1 bindingBy similarityAdd BLAST176
Regioni311 – 3246.5 X 2 AA tandem repeats of R-GAdd BLAST14

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi347 – 351PXLXP motif, interaction with host ZMYND11By similarity5
Motifi401 – 405PXLXP motif, interaction with host ZMYND11By similarity5

Sequence similaritiesi

Belongs to the herpesviridae EBNA2 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

OrthoDBiVOG090000FZ.

Sequencei

Sequence statusi: Complete.

Q3KSV2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTFYLALHG GQTYHLIVDT DSVGNPSLSV IPSNPYQEQL SDTPLIPLTI
60 70 80 90 100
FVGENTGVPP PPPPPPQRRD AWTQEPSPLD WDPLGYDVGH GPLASAMRML
110 120 130 140 150
WMANYIVRQS RGDRGLILPQ GPQTAPQAML VQPHVPPLRP TAPTILSPLS
160 170 180 190 200
QPRLTPPQPL MMPPRPTPPT PLPPATLTVP PRPTRPTTLP PTPLLTVLQR
210 220 230 240 250
PTELQPTPSP PRMHLPVLHV PDQSMHPLTH QSTPNDPDSP EPRSPTVFYN
260 270 280 290 300
IPPMPLPPSQ LPPPAAPAQP PPGVINDQQL HHLPSGPPWW PPICDPPQPS
310 320 330 340 350
KTQGQSRGQS RGRGRGRGRG RGKSRDKQRK PGGPWRPEPN TSSPSMPELS
360 370 380 390 400
PVLGLHQGQG AGDSPTPGPS NAAPVCRNSH TATPNVSPIH EPESHNSPEA
410 420 430 440 450
PILFPDDWYP PSIDPADLDE SWDYIFETTE SPSSDEDYVE GPSKRPRPSI

Q
Length:451
Mass (Da):49,042
Last modified:November 8, 2005 - v1
Checksum:iFF12A76055A91C9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY961628 Genomic DNA. Translation: AAY41099.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY961628 Genomic DNA. Translation: AAY41099.1.

3D structure databases

SMRiQ3KSV2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3KSV2. 13 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiVOG090000FZ.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiEBNA2_EBVG
AccessioniPrimary (citable) accession number: Q3KSV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: November 8, 2005
Last modified: March 15, 2017
This is version 43 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.