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Q3KSU8

- DEN_EBVG

UniProt

Q3KSU8 - DEN_EBVG

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Protein
Deneddylase
Gene
BPLF1
Organism
Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei61 – 611 By similarity
Active sitei195 – 1951 Reviewed prediction

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Large tegument protein
Short name:
LTP
Gene namesi
ORF Names:BPLF1
OrganismiEpstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10376 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007641: Genome

Subcellular locationi

Virion tegument By similarity. Host nucleus By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-SubCell
  2. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 31763176Deneddylase
PRO_0000375970Add
BLAST

Proteomic databases

PRIDEiQ3KSU8.

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Protein-protein interaction databases

IntActiQ3KSU8. 14 interactions.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 258218Peptidase C76
Add
BLAST
Repeati335 – 33951
Repeati340 – 34452
Repeati345 – 34953
Repeati350 – 35454
Repeati355 – 35955
Repeati360 – 36456; approximate
Repeati365 – 36957; approximate
Repeati370 – 37458
Repeati375 – 37959
Repeati380 – 384510; approximate

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 269269Deubiquitination activity By similarity
Add
BLAST
Regioni335 – 3845010 X 5 AA approximate repeats of P-A-S-A-A
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1127 – 115731 Reviewed prediction
Add
BLAST
Coiled coili1421 – 146545 Reviewed prediction
Add
BLAST
Coiled coili1733 – 176230 Reviewed prediction
Add
BLAST
Coiled coili1902 – 193130 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi422 – 4287Nuclear localization signal By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi336 – 38651Ala-rich
Add
BLAST
Compositional biasi537 – 5404Poly-Ala
Compositional biasi1383 – 13864Poly-Asn
Compositional biasi1502 – 15109Poly-Ala
Compositional biasi1585 – 15906Poly-Gly
Compositional biasi1683 – 16864Poly-Pro
Compositional biasi1857 – 18604Poly-Ala
Compositional biasi2112 – 21154Poly-Arg
Compositional biasi2127 – 21304Poly-Ala
Compositional biasi2872 – 2975104Ala-rich
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3KSU8-1 [UniParc]FASTAAdd to Basket

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MSNGDWGQSQ RPRGTGPMRG IRTMDVNAPG GGSGGSALRI LGTASCNQAH     50
CKFGRFAGIQ CVSNCVLYLV KSFLAGRPLT SRPELDEVLD EGARLDALMR 100
QSGILKGHEM AQLTDVPSSV VLRGGGRVHI YRSAEIFGLV LFPAQIANSA 150
VVQSLAEVLH GSYNGVAQFI LYICDIYAGG IIIETDGSFY LFDPHCQKDA 200
APGTPAHVRV STYAHDILQY VGAPGAQYTC VHLYFLPEAF ETEDPRIFML 250
EHYGVYDFYE ANGSGFDLVG PELVSSDGEA AGTPDADSSP PVMLPFERRI 300
IPYNLRPLPS RSFTSDSFPA ARYSPAKTNS PPPSPASAAP ASAAPASAAP 350
ASAAPASAAQ ASVAPASVAP ASAAPASAAP DSAAPASSPP LFIPIPGLGH 400
TPGVPAPSTP PRASGSAAPQ TPKRKKGLGK DSPHKKPTSG RRLPLSSTTD 450
TEDDQLPRPP VPPHRPPSAA RLPPPVIPIP HQSPPASPTP RRAPVSTIAP 500
SVTPSPRLPL QIPIPLPQAA PSNPEIPLTT PSPSPTAAAA PTATTLSPPP 550
TQQQPLQSAA PAPSPPPTQQ QPPQSAAPAP SPLLPQQQPP PSAAPAPSPL 600
LPQQQPPPSA ARAPSPLPPQ QQPLPSATPA PPPAQQLPPS ATTLEPEKNN 650
PSAADRAGTE ISPSPPFGQQ PSFGDDASGG SGLVRYLSDL EEPFLSMSDS 700
EEAESDLASD IPTTEDEDMF EDEVFSNSLE SGSSAPTSPI TLDTARSQYY 750
QTTFDIETPE MDFVPLESNI ARIAGHTYQE QAIVYDPASN REVPEADALS 800
MIDYLLVTVV LEQGLIRSRD RSAVLNLLEF LKDWSGHLQV PTLDLEQLLT 850
SELNIQNLAN MLSENKGRAG EFHEHLAAKL EACLPSLATK DAVRVDAGAK 900
MLAEIPQLAE SDDGKFDLEA ARRRLTDLLS GGDQEGEEGG GEPEDHSIYR 950
GPHVDVPLVL DDESWKRLLS LAEAARTAVA RQQAGVDEED VRFLALLTAI 1000
EYGAPPAASV PPFVHNVAVR SKNAALHVRR CTADIRDKVA SAASDYLSYL 1050
EDPSLPTVMD FDDLLTHLRH TCQIIASLPL LNIRYTSIEW DYRELLYLGT 1100
ALSDMSGIPW PLERVEEDDP SIAPLPEFET VAKKQKELET TRENEKRLRT 1150
ILDDIEAMLG LAGVASAPGA PISPASPSAT PANHDNPEAT PPLADTAALT 1200
IPVIEKYIAN AGSIVGAAKN PTYIRLRDTI QQIVRSKKYL MNILKSITFY 1250
TIDNYIASFE ESIDHLYRDL PVLDPEVQDG IDRILDPMVS EALHTFEMGN 1300
RLTLEPARLV ALQNFATHST LKETAAAVNL LPGLLAVYDA TVTGQAPEDA 1350
LRLLSGLQNQ LSQTLIPGKL KKRFLSYLQK LKNNNNDQLR QKEVQAWRLE 1400
AEGFKPATEE QLEAFLDTAP NKELKRQYEK KLRQLMETGR KEKEKLREQE 1450
DKERRERRAR EANEAWARIR KALGARPEPA PTSPDDWNTL LASLLPDNTD 1500
SAAAAAAAVA RNTDILDSLT QILAAMLLGI TRVRRERLRS LLVDDGGAAE 1550
RMEAAEPGWF TDIETGPLAR LDAWPATPAA TAKEGGGGRG AEEAAGALFR 1600
ARTAADAIRS ALAQTRQALQ SPDMKSAVVN TDLEAPYAEY ERGLAGLLEK 1650
RRAAEAALTA IVSEYVDRTL PEATNDPGQA NLPPPPTIPQ ATAPPRLASD 1700
LALWPKKPQL LTRRERDDLL QATGDFFSEL LTEAEAAEVR ALEEQVRESQ 1750
TLMAKAHEMA ASTRRGFHTA LEAVLSRSRD EAPDDELRSL LPSPPKAPVQ 1800
APLEAALARA AAGNGSWPYR KSLAAAKWIR GICEAVRGLS EGALALAGGA 1850
GAWLNLAAAA DGEIHELTRL LEVEGMAQNS MDGMEELRLA LATLDPKRVA 1900
GGKETVADWK RRLSRLEAII QEAQEESQLQ GTLQDLVTQA RGHTDPRQLK 1950
IVVEAARGLA LGASAGSQYA LLKDKLLRYA SAKQSFLAFY ETAQPTVFVK 2000
HPLTNNLPLL ITISAPPTGW GNGAPTRRAQ FLAAAGPAKY AGTLWLETES 2050
PCDPLNPAYV SADTQEPLNY IPVYHNFLEY VMPTVLENPE AFSLTPAGRP 2100
QAIGPPQDDQ ERRRRTLASV ASARLSAAAA DSYWDTWPDV ESNAGELLRE 2150
YVSAPKALME DLADNPIVAM TLLAHASLIA SRNHPPYPAP ATDREVILLE 2200
QREIMALLVG THPAYAAAFL GAPSFYAGLG LVSALARDGG LGDLLSDSVL 2250
TYRLVRSPAS GRGGMPSTTR GSDDGEDARR LTRHRIAGPP TGFIFFQDAW 2300
EEMDTRAALW PHPEFLGLVH NQSTARARAC MLLLARRCFA PEALQQLWHS 2350
LRPLEGPVAF QDYLRDFVKQ AYTRGEELPR AEGLEVPRET PSSYGTVTGR 2400
ALRNLMPYGT PITGPKRGSG DTIPVSVFEA AVAAAFLGRP LTLFVSSQYL 2450
FNLKTLGQVR VVAPLLYCDG HSEPFRSLVE TISLNFLQDL DGYSESFEPE 2500
MSIFARQAVW LRELLTEARA AKPKEARPPT VAILANRKNI IWKCFTYRHN 2550
LPDVQFYFNA AGASRWPTDV LNPSFYEHED PPLPVGYQLP PNPRNVQELF 2600
SGFPPRVGHG LVSGDGFQSA DNTPASSDRL QQLGGGETDQ GEEGSTTAES 2650
EASGPPSPQS PLLEKVAPGR PRDWLSPTSS PRDVTVTPGL AAPITLPGPR 2700
LMARPYFGAE TRASESPDRS PGTSPRPWPK DSLELLPQPA PQQPPSSPWA 2750
SEQGPIVYTL SPHSTPSTAS GSQKKHTIQI PGLVPSQKPS YPPSAPYKPG 2800
QSTGGIAPTP SAASLTTFGL QPQDTQASSQ DPPYGHSIMQ REKKQQGGRE 2850
EAAEIRPSAT RLPTAVGLRP RAPVVAAGAA ASATPAFDPG EAPSGLPIPQ 2900
APALGSGLAA PAHTPVGALA PSPQKTQAQR PQDAAALPTP TIKAVGARPV 2950
PKATGALAAG ARPRGQPTAA PPSAASPPRV SLPVRSRQQQ SPAIPLPPMH 3000
SGSEPGARPE VRLSQYRHAG PQTYTVQKEA PPSAASQLPK MPKCKDSMYY 3050
PPSGSARYPA PFQALSFSQS VASPAPSSDQ TTLLWNTPSV VTQFLSIEDI 3100
IREVVTGGST SGDLVVPSGS PSSLSTAAPE QDLRYSLTLS QASRVLSRFV 3150
SQLRRKLERS THRLIADLER LKFLYL 3176
Length:3,176
Mass (Da):340,605
Last modified:November 8, 2005 - v1
Checksum:i1D4CDE9D746627D6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY961628 Genomic DNA. Translation: AAY41102.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY961628 Genomic DNA. Translation: AAY41102.1 .

3D structure databases

ModBasei Search...

Protein-protein interaction databases

IntActi Q3KSU8. 14 interactions.

Proteomic databases

PRIDEi Q3KSU8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genomic sequence analysis of Epstein-Barr virus strain GD1 from a nasopharyngeal carcinoma patient."
    Zeng M.-S., Li D.-J., Liu Q.-L., Song L.-B., Li M.-Z., Zhang R.-H., Yu X.-J., Wang H.-M., Ernberg I., Zeng Y.-X.
    J. Virol. 79:15323-15330(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_EBVG
AccessioniPrimary (citable) accession number: Q3KSU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 8, 2005
Last modified: May 14, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi