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Q3KSU8

- DEN_EBVG

UniProt

Q3KSU8 - DEN_EBVG

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Protein

Deneddylase

Gene

BPLF1

Organism
Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei61 – 611PROSITE-ProRule annotation
Active sitei195 – 1951PROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Large tegument protein
Short name:
LTP
Gene namesi
ORF Names:BPLF1
OrganismiEpstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10376 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007641: Genome

Subcellular locationi

Virion tegument By similarity. Host nucleus By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
  2. viral tegument Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 31763176DeneddylasePRO_0000375970Add
BLAST

Proteomic databases

PRIDEiQ3KSU8.

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

Protein-protein interaction databases

IntActiQ3KSU8. 14 interactions.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 258218Peptidase C76PROSITE-ProRule annotationAdd
BLAST
Repeati335 – 33951
Repeati340 – 34452
Repeati345 – 34953
Repeati350 – 35454
Repeati355 – 35955
Repeati360 – 36456; approximate
Repeati365 – 36957; approximate
Repeati370 – 37458
Repeati375 – 37959
Repeati380 – 384510; approximate

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 269269Deubiquitination activityBy similarityAdd
BLAST
Regioni335 – 3845010 X 5 AA approximate repeats of P-A-S-A-AAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1127 – 115731Sequence AnalysisAdd
BLAST
Coiled coili1421 – 146545Sequence AnalysisAdd
BLAST
Coiled coili1733 – 176230Sequence AnalysisAdd
BLAST
Coiled coili1902 – 193130Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi422 – 4287Nuclear localization signalBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi336 – 38651Ala-richAdd
BLAST
Compositional biasi537 – 5404Poly-Ala
Compositional biasi1383 – 13864Poly-Asn
Compositional biasi1502 – 15109Poly-Ala
Compositional biasi1585 – 15906Poly-Gly
Compositional biasi1683 – 16864Poly-Pro
Compositional biasi1857 – 18604Poly-Ala
Compositional biasi2112 – 21154Poly-Arg
Compositional biasi2127 – 21304Poly-Ala
Compositional biasi2872 – 2975104Ala-richAdd
BLAST

Sequence similaritiesi

Belongs to the herpesviridae deneddylase family.Curated
Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3KSU8-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MSNGDWGQSQ RPRGTGPMRG IRTMDVNAPG GGSGGSALRI LGTASCNQAH
60 70 80 90 100
CKFGRFAGIQ CVSNCVLYLV KSFLAGRPLT SRPELDEVLD EGARLDALMR
110 120 130 140 150
QSGILKGHEM AQLTDVPSSV VLRGGGRVHI YRSAEIFGLV LFPAQIANSA
160 170 180 190 200
VVQSLAEVLH GSYNGVAQFI LYICDIYAGG IIIETDGSFY LFDPHCQKDA
210 220 230 240 250
APGTPAHVRV STYAHDILQY VGAPGAQYTC VHLYFLPEAF ETEDPRIFML
260 270 280 290 300
EHYGVYDFYE ANGSGFDLVG PELVSSDGEA AGTPDADSSP PVMLPFERRI
310 320 330 340 350
IPYNLRPLPS RSFTSDSFPA ARYSPAKTNS PPPSPASAAP ASAAPASAAP
360 370 380 390 400
ASAAPASAAQ ASVAPASVAP ASAAPASAAP DSAAPASSPP LFIPIPGLGH
410 420 430 440 450
TPGVPAPSTP PRASGSAAPQ TPKRKKGLGK DSPHKKPTSG RRLPLSSTTD
460 470 480 490 500
TEDDQLPRPP VPPHRPPSAA RLPPPVIPIP HQSPPASPTP RRAPVSTIAP
510 520 530 540 550
SVTPSPRLPL QIPIPLPQAA PSNPEIPLTT PSPSPTAAAA PTATTLSPPP
560 570 580 590 600
TQQQPLQSAA PAPSPPPTQQ QPPQSAAPAP SPLLPQQQPP PSAAPAPSPL
610 620 630 640 650
LPQQQPPPSA ARAPSPLPPQ QQPLPSATPA PPPAQQLPPS ATTLEPEKNN
660 670 680 690 700
PSAADRAGTE ISPSPPFGQQ PSFGDDASGG SGLVRYLSDL EEPFLSMSDS
710 720 730 740 750
EEAESDLASD IPTTEDEDMF EDEVFSNSLE SGSSAPTSPI TLDTARSQYY
760 770 780 790 800
QTTFDIETPE MDFVPLESNI ARIAGHTYQE QAIVYDPASN REVPEADALS
810 820 830 840 850
MIDYLLVTVV LEQGLIRSRD RSAVLNLLEF LKDWSGHLQV PTLDLEQLLT
860 870 880 890 900
SELNIQNLAN MLSENKGRAG EFHEHLAAKL EACLPSLATK DAVRVDAGAK
910 920 930 940 950
MLAEIPQLAE SDDGKFDLEA ARRRLTDLLS GGDQEGEEGG GEPEDHSIYR
960 970 980 990 1000
GPHVDVPLVL DDESWKRLLS LAEAARTAVA RQQAGVDEED VRFLALLTAI
1010 1020 1030 1040 1050
EYGAPPAASV PPFVHNVAVR SKNAALHVRR CTADIRDKVA SAASDYLSYL
1060 1070 1080 1090 1100
EDPSLPTVMD FDDLLTHLRH TCQIIASLPL LNIRYTSIEW DYRELLYLGT
1110 1120 1130 1140 1150
ALSDMSGIPW PLERVEEDDP SIAPLPEFET VAKKQKELET TRENEKRLRT
1160 1170 1180 1190 1200
ILDDIEAMLG LAGVASAPGA PISPASPSAT PANHDNPEAT PPLADTAALT
1210 1220 1230 1240 1250
IPVIEKYIAN AGSIVGAAKN PTYIRLRDTI QQIVRSKKYL MNILKSITFY
1260 1270 1280 1290 1300
TIDNYIASFE ESIDHLYRDL PVLDPEVQDG IDRILDPMVS EALHTFEMGN
1310 1320 1330 1340 1350
RLTLEPARLV ALQNFATHST LKETAAAVNL LPGLLAVYDA TVTGQAPEDA
1360 1370 1380 1390 1400
LRLLSGLQNQ LSQTLIPGKL KKRFLSYLQK LKNNNNDQLR QKEVQAWRLE
1410 1420 1430 1440 1450
AEGFKPATEE QLEAFLDTAP NKELKRQYEK KLRQLMETGR KEKEKLREQE
1460 1470 1480 1490 1500
DKERRERRAR EANEAWARIR KALGARPEPA PTSPDDWNTL LASLLPDNTD
1510 1520 1530 1540 1550
SAAAAAAAVA RNTDILDSLT QILAAMLLGI TRVRRERLRS LLVDDGGAAE
1560 1570 1580 1590 1600
RMEAAEPGWF TDIETGPLAR LDAWPATPAA TAKEGGGGRG AEEAAGALFR
1610 1620 1630 1640 1650
ARTAADAIRS ALAQTRQALQ SPDMKSAVVN TDLEAPYAEY ERGLAGLLEK
1660 1670 1680 1690 1700
RRAAEAALTA IVSEYVDRTL PEATNDPGQA NLPPPPTIPQ ATAPPRLASD
1710 1720 1730 1740 1750
LALWPKKPQL LTRRERDDLL QATGDFFSEL LTEAEAAEVR ALEEQVRESQ
1760 1770 1780 1790 1800
TLMAKAHEMA ASTRRGFHTA LEAVLSRSRD EAPDDELRSL LPSPPKAPVQ
1810 1820 1830 1840 1850
APLEAALARA AAGNGSWPYR KSLAAAKWIR GICEAVRGLS EGALALAGGA
1860 1870 1880 1890 1900
GAWLNLAAAA DGEIHELTRL LEVEGMAQNS MDGMEELRLA LATLDPKRVA
1910 1920 1930 1940 1950
GGKETVADWK RRLSRLEAII QEAQEESQLQ GTLQDLVTQA RGHTDPRQLK
1960 1970 1980 1990 2000
IVVEAARGLA LGASAGSQYA LLKDKLLRYA SAKQSFLAFY ETAQPTVFVK
2010 2020 2030 2040 2050
HPLTNNLPLL ITISAPPTGW GNGAPTRRAQ FLAAAGPAKY AGTLWLETES
2060 2070 2080 2090 2100
PCDPLNPAYV SADTQEPLNY IPVYHNFLEY VMPTVLENPE AFSLTPAGRP
2110 2120 2130 2140 2150
QAIGPPQDDQ ERRRRTLASV ASARLSAAAA DSYWDTWPDV ESNAGELLRE
2160 2170 2180 2190 2200
YVSAPKALME DLADNPIVAM TLLAHASLIA SRNHPPYPAP ATDREVILLE
2210 2220 2230 2240 2250
QREIMALLVG THPAYAAAFL GAPSFYAGLG LVSALARDGG LGDLLSDSVL
2260 2270 2280 2290 2300
TYRLVRSPAS GRGGMPSTTR GSDDGEDARR LTRHRIAGPP TGFIFFQDAW
2310 2320 2330 2340 2350
EEMDTRAALW PHPEFLGLVH NQSTARARAC MLLLARRCFA PEALQQLWHS
2360 2370 2380 2390 2400
LRPLEGPVAF QDYLRDFVKQ AYTRGEELPR AEGLEVPRET PSSYGTVTGR
2410 2420 2430 2440 2450
ALRNLMPYGT PITGPKRGSG DTIPVSVFEA AVAAAFLGRP LTLFVSSQYL
2460 2470 2480 2490 2500
FNLKTLGQVR VVAPLLYCDG HSEPFRSLVE TISLNFLQDL DGYSESFEPE
2510 2520 2530 2540 2550
MSIFARQAVW LRELLTEARA AKPKEARPPT VAILANRKNI IWKCFTYRHN
2560 2570 2580 2590 2600
LPDVQFYFNA AGASRWPTDV LNPSFYEHED PPLPVGYQLP PNPRNVQELF
2610 2620 2630 2640 2650
SGFPPRVGHG LVSGDGFQSA DNTPASSDRL QQLGGGETDQ GEEGSTTAES
2660 2670 2680 2690 2700
EASGPPSPQS PLLEKVAPGR PRDWLSPTSS PRDVTVTPGL AAPITLPGPR
2710 2720 2730 2740 2750
LMARPYFGAE TRASESPDRS PGTSPRPWPK DSLELLPQPA PQQPPSSPWA
2760 2770 2780 2790 2800
SEQGPIVYTL SPHSTPSTAS GSQKKHTIQI PGLVPSQKPS YPPSAPYKPG
2810 2820 2830 2840 2850
QSTGGIAPTP SAASLTTFGL QPQDTQASSQ DPPYGHSIMQ REKKQQGGRE
2860 2870 2880 2890 2900
EAAEIRPSAT RLPTAVGLRP RAPVVAAGAA ASATPAFDPG EAPSGLPIPQ
2910 2920 2930 2940 2950
APALGSGLAA PAHTPVGALA PSPQKTQAQR PQDAAALPTP TIKAVGARPV
2960 2970 2980 2990 3000
PKATGALAAG ARPRGQPTAA PPSAASPPRV SLPVRSRQQQ SPAIPLPPMH
3010 3020 3030 3040 3050
SGSEPGARPE VRLSQYRHAG PQTYTVQKEA PPSAASQLPK MPKCKDSMYY
3060 3070 3080 3090 3100
PPSGSARYPA PFQALSFSQS VASPAPSSDQ TTLLWNTPSV VTQFLSIEDI
3110 3120 3130 3140 3150
IREVVTGGST SGDLVVPSGS PSSLSTAAPE QDLRYSLTLS QASRVLSRFV
3160 3170
SQLRRKLERS THRLIADLER LKFLYL
Length:3,176
Mass (Da):340,605
Last modified:November 8, 2005 - v1
Checksum:i1D4CDE9D746627D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY961628 Genomic DNA. Translation: AAY41102.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY961628 Genomic DNA. Translation: AAY41102.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q3KSU8. 14 interactions.

Proteomic databases

PRIDEi Q3KSU8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genomic sequence analysis of Epstein-Barr virus strain GD1 from a nasopharyngeal carcinoma patient."
    Zeng M.-S., Li D.-J., Liu Q.-L., Song L.-B., Li M.-Z., Zhang R.-H., Yu X.-J., Wang H.-M., Ernberg I., Zeng Y.-X.
    J. Virol. 79:15323-15330(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_EBVG
AccessioniPrimary (citable) accession number: Q3KSU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 8, 2005
Last modified: October 29, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3