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Q3KSU8

- DEN_EBVG

UniProt

Q3KSU8 - DEN_EBVG

Protein

Deneddylase

Gene

BPLF1

Organism
Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei61 – 611PROSITE-ProRule annotation
    Active sitei195 – 1951PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
    Alternative name(s):
    Large tegument protein
    Short name:
    LTP
    Gene namesi
    ORF Names:BPLF1
    OrganismiEpstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
    Taxonomic identifieri10376 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000007641: Genome

    Subcellular locationi

    Virion tegument By similarity. Host nucleus By similarity

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell
    2. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 31763176DeneddylasePRO_0000375970Add
    BLAST

    Proteomic databases

    PRIDEiQ3KSU8.

    Interactioni

    Subunit structurei

    Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

    Protein-protein interaction databases

    IntActiQ3KSU8. 14 interactions.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 258218Peptidase C76PROSITE-ProRule annotationAdd
    BLAST
    Repeati335 – 33951
    Repeati340 – 34452
    Repeati345 – 34953
    Repeati350 – 35454
    Repeati355 – 35955
    Repeati360 – 36456; approximate
    Repeati365 – 36957; approximate
    Repeati370 – 37458
    Repeati375 – 37959
    Repeati380 – 384510; approximate

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 269269Deubiquitination activityBy similarityAdd
    BLAST
    Regioni335 – 3845010 X 5 AA approximate repeats of P-A-S-A-AAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1127 – 115731Sequence AnalysisAdd
    BLAST
    Coiled coili1421 – 146545Sequence AnalysisAdd
    BLAST
    Coiled coili1733 – 176230Sequence AnalysisAdd
    BLAST
    Coiled coili1902 – 193130Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi422 – 4287Nuclear localization signalBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi336 – 38651Ala-richAdd
    BLAST
    Compositional biasi537 – 5404Poly-Ala
    Compositional biasi1383 – 13864Poly-Asn
    Compositional biasi1502 – 15109Poly-Ala
    Compositional biasi1585 – 15906Poly-Gly
    Compositional biasi1683 – 16864Poly-Pro
    Compositional biasi1857 – 18604Poly-Ala
    Compositional biasi2112 – 21154Poly-Arg
    Compositional biasi2127 – 21304Poly-Ala
    Compositional biasi2872 – 2975104Ala-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the herpesviridae deneddylase family.Curated
    Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Family and domain databases

    InterProiIPR006928. Pept_C76_UL36-USP.
    [Graphical view]
    PfamiPF04843. Herpes_teg_N. 1 hit.
    [Graphical view]
    PROSITEiPS51521. HTUSP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3KSU8-1 [UniParc]FASTAAdd to Basket

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    MSNGDWGQSQ RPRGTGPMRG IRTMDVNAPG GGSGGSALRI LGTASCNQAH     50
    CKFGRFAGIQ CVSNCVLYLV KSFLAGRPLT SRPELDEVLD EGARLDALMR 100
    QSGILKGHEM AQLTDVPSSV VLRGGGRVHI YRSAEIFGLV LFPAQIANSA 150
    VVQSLAEVLH GSYNGVAQFI LYICDIYAGG IIIETDGSFY LFDPHCQKDA 200
    APGTPAHVRV STYAHDILQY VGAPGAQYTC VHLYFLPEAF ETEDPRIFML 250
    EHYGVYDFYE ANGSGFDLVG PELVSSDGEA AGTPDADSSP PVMLPFERRI 300
    IPYNLRPLPS RSFTSDSFPA ARYSPAKTNS PPPSPASAAP ASAAPASAAP 350
    ASAAPASAAQ ASVAPASVAP ASAAPASAAP DSAAPASSPP LFIPIPGLGH 400
    TPGVPAPSTP PRASGSAAPQ TPKRKKGLGK DSPHKKPTSG RRLPLSSTTD 450
    TEDDQLPRPP VPPHRPPSAA RLPPPVIPIP HQSPPASPTP RRAPVSTIAP 500
    SVTPSPRLPL QIPIPLPQAA PSNPEIPLTT PSPSPTAAAA PTATTLSPPP 550
    TQQQPLQSAA PAPSPPPTQQ QPPQSAAPAP SPLLPQQQPP PSAAPAPSPL 600
    LPQQQPPPSA ARAPSPLPPQ QQPLPSATPA PPPAQQLPPS ATTLEPEKNN 650
    PSAADRAGTE ISPSPPFGQQ PSFGDDASGG SGLVRYLSDL EEPFLSMSDS 700
    EEAESDLASD IPTTEDEDMF EDEVFSNSLE SGSSAPTSPI TLDTARSQYY 750
    QTTFDIETPE MDFVPLESNI ARIAGHTYQE QAIVYDPASN REVPEADALS 800
    MIDYLLVTVV LEQGLIRSRD RSAVLNLLEF LKDWSGHLQV PTLDLEQLLT 850
    SELNIQNLAN MLSENKGRAG EFHEHLAAKL EACLPSLATK DAVRVDAGAK 900
    MLAEIPQLAE SDDGKFDLEA ARRRLTDLLS GGDQEGEEGG GEPEDHSIYR 950
    GPHVDVPLVL DDESWKRLLS LAEAARTAVA RQQAGVDEED VRFLALLTAI 1000
    EYGAPPAASV PPFVHNVAVR SKNAALHVRR CTADIRDKVA SAASDYLSYL 1050
    EDPSLPTVMD FDDLLTHLRH TCQIIASLPL LNIRYTSIEW DYRELLYLGT 1100
    ALSDMSGIPW PLERVEEDDP SIAPLPEFET VAKKQKELET TRENEKRLRT 1150
    ILDDIEAMLG LAGVASAPGA PISPASPSAT PANHDNPEAT PPLADTAALT 1200
    IPVIEKYIAN AGSIVGAAKN PTYIRLRDTI QQIVRSKKYL MNILKSITFY 1250
    TIDNYIASFE ESIDHLYRDL PVLDPEVQDG IDRILDPMVS EALHTFEMGN 1300
    RLTLEPARLV ALQNFATHST LKETAAAVNL LPGLLAVYDA TVTGQAPEDA 1350
    LRLLSGLQNQ LSQTLIPGKL KKRFLSYLQK LKNNNNDQLR QKEVQAWRLE 1400
    AEGFKPATEE QLEAFLDTAP NKELKRQYEK KLRQLMETGR KEKEKLREQE 1450
    DKERRERRAR EANEAWARIR KALGARPEPA PTSPDDWNTL LASLLPDNTD 1500
    SAAAAAAAVA RNTDILDSLT QILAAMLLGI TRVRRERLRS LLVDDGGAAE 1550
    RMEAAEPGWF TDIETGPLAR LDAWPATPAA TAKEGGGGRG AEEAAGALFR 1600
    ARTAADAIRS ALAQTRQALQ SPDMKSAVVN TDLEAPYAEY ERGLAGLLEK 1650
    RRAAEAALTA IVSEYVDRTL PEATNDPGQA NLPPPPTIPQ ATAPPRLASD 1700
    LALWPKKPQL LTRRERDDLL QATGDFFSEL LTEAEAAEVR ALEEQVRESQ 1750
    TLMAKAHEMA ASTRRGFHTA LEAVLSRSRD EAPDDELRSL LPSPPKAPVQ 1800
    APLEAALARA AAGNGSWPYR KSLAAAKWIR GICEAVRGLS EGALALAGGA 1850
    GAWLNLAAAA DGEIHELTRL LEVEGMAQNS MDGMEELRLA LATLDPKRVA 1900
    GGKETVADWK RRLSRLEAII QEAQEESQLQ GTLQDLVTQA RGHTDPRQLK 1950
    IVVEAARGLA LGASAGSQYA LLKDKLLRYA SAKQSFLAFY ETAQPTVFVK 2000
    HPLTNNLPLL ITISAPPTGW GNGAPTRRAQ FLAAAGPAKY AGTLWLETES 2050
    PCDPLNPAYV SADTQEPLNY IPVYHNFLEY VMPTVLENPE AFSLTPAGRP 2100
    QAIGPPQDDQ ERRRRTLASV ASARLSAAAA DSYWDTWPDV ESNAGELLRE 2150
    YVSAPKALME DLADNPIVAM TLLAHASLIA SRNHPPYPAP ATDREVILLE 2200
    QREIMALLVG THPAYAAAFL GAPSFYAGLG LVSALARDGG LGDLLSDSVL 2250
    TYRLVRSPAS GRGGMPSTTR GSDDGEDARR LTRHRIAGPP TGFIFFQDAW 2300
    EEMDTRAALW PHPEFLGLVH NQSTARARAC MLLLARRCFA PEALQQLWHS 2350
    LRPLEGPVAF QDYLRDFVKQ AYTRGEELPR AEGLEVPRET PSSYGTVTGR 2400
    ALRNLMPYGT PITGPKRGSG DTIPVSVFEA AVAAAFLGRP LTLFVSSQYL 2450
    FNLKTLGQVR VVAPLLYCDG HSEPFRSLVE TISLNFLQDL DGYSESFEPE 2500
    MSIFARQAVW LRELLTEARA AKPKEARPPT VAILANRKNI IWKCFTYRHN 2550
    LPDVQFYFNA AGASRWPTDV LNPSFYEHED PPLPVGYQLP PNPRNVQELF 2600
    SGFPPRVGHG LVSGDGFQSA DNTPASSDRL QQLGGGETDQ GEEGSTTAES 2650
    EASGPPSPQS PLLEKVAPGR PRDWLSPTSS PRDVTVTPGL AAPITLPGPR 2700
    LMARPYFGAE TRASESPDRS PGTSPRPWPK DSLELLPQPA PQQPPSSPWA 2750
    SEQGPIVYTL SPHSTPSTAS GSQKKHTIQI PGLVPSQKPS YPPSAPYKPG 2800
    QSTGGIAPTP SAASLTTFGL QPQDTQASSQ DPPYGHSIMQ REKKQQGGRE 2850
    EAAEIRPSAT RLPTAVGLRP RAPVVAAGAA ASATPAFDPG EAPSGLPIPQ 2900
    APALGSGLAA PAHTPVGALA PSPQKTQAQR PQDAAALPTP TIKAVGARPV 2950
    PKATGALAAG ARPRGQPTAA PPSAASPPRV SLPVRSRQQQ SPAIPLPPMH 3000
    SGSEPGARPE VRLSQYRHAG PQTYTVQKEA PPSAASQLPK MPKCKDSMYY 3050
    PPSGSARYPA PFQALSFSQS VASPAPSSDQ TTLLWNTPSV VTQFLSIEDI 3100
    IREVVTGGST SGDLVVPSGS PSSLSTAAPE QDLRYSLTLS QASRVLSRFV 3150
    SQLRRKLERS THRLIADLER LKFLYL 3176
    Length:3,176
    Mass (Da):340,605
    Last modified:November 8, 2005 - v1
    Checksum:i1D4CDE9D746627D6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY961628 Genomic DNA. Translation: AAY41102.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY961628 Genomic DNA. Translation: AAY41102.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q3KSU8. 14 interactions.

    Proteomic databases

    PRIDEi Q3KSU8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR006928. Pept_C76_UL36-USP.
    [Graphical view ]
    Pfami PF04843. Herpes_teg_N. 1 hit.
    [Graphical view ]
    PROSITEi PS51521. HTUSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequence analysis of Epstein-Barr virus strain GD1 from a nasopharyngeal carcinoma patient."
      Zeng M.-S., Li D.-J., Liu Q.-L., Song L.-B., Li M.-Z., Zhang R.-H., Yu X.-J., Wang H.-M., Ernberg I., Zeng Y.-X.
      J. Virol. 79:15323-15330(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiDEN_EBVG
    AccessioniPrimary (citable) accession number: Q3KSU8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 26, 2009
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3