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Q3KSU8 (DEN_EBVG) Reviewed, UniProtKB/Swiss-Prot

Last modified June 26, 2013. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deneddylase

EC=3.4.19.12
EC=3.4.22.-
Alternative name(s):
Large tegument protein
Short name=LTP
Gene names
ORF Names:BPLF1
OrganismEpstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4) [Complete proteome]
Taxonomic identifier10376 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length3176 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Subcellular location

Virion tegument By similarity. Host nucleus By similarity.

Sequence similarities

Belongs to the herpesviridae deneddylase family.

Contains 1 peptidase C76 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 31763176Deneddylase
PRO_0000375970

Regions

Domain41 – 258218Peptidase C76
Repeat335 – 33951
Repeat340 – 34452
Repeat345 – 34953
Repeat350 – 35454
Repeat355 – 35955
Repeat360 – 36456; approximate
Repeat365 – 36957; approximate
Repeat370 – 37458
Repeat375 – 37959
Repeat380 – 384510; approximate
Region1 – 269269Deubiquitination activity By similarity
Region335 – 3845010 X 5 AA approximate repeats of P-A-S-A-A
Coiled coil1127 – 115731 Potential
Coiled coil1421 – 146545 Potential
Coiled coil1733 – 176230 Potential
Coiled coil1902 – 193130 Potential
Motif422 – 4287Nuclear localization signal By similarity
Compositional bias336 – 38651Ala-rich
Compositional bias537 – 5404Poly-Ala
Compositional bias1383 – 13864Poly-Asn
Compositional bias1502 – 15109Poly-Ala
Compositional bias1585 – 15906Poly-Gly
Compositional bias1683 – 16864Poly-Pro
Compositional bias1857 – 18604Poly-Ala
Compositional bias2112 – 21154Poly-Arg
Compositional bias2127 – 21304Poly-Ala
Compositional bias2872 – 2975104Ala-rich

Sites

Active site611 By similarity
Active site1951 Potential

Sequences

Sequence LengthMass (Da)Tools
Q3KSU8 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 1D4CDE9D746627D6

FASTA3,176340,605
        10         20         30         40         50         60 
MSNGDWGQSQ RPRGTGPMRG IRTMDVNAPG GGSGGSALRI LGTASCNQAH CKFGRFAGIQ 

        70         80         90        100        110        120 
CVSNCVLYLV KSFLAGRPLT SRPELDEVLD EGARLDALMR QSGILKGHEM AQLTDVPSSV 

       130        140        150        160        170        180 
VLRGGGRVHI YRSAEIFGLV LFPAQIANSA VVQSLAEVLH GSYNGVAQFI LYICDIYAGG 

       190        200        210        220        230        240 
IIIETDGSFY LFDPHCQKDA APGTPAHVRV STYAHDILQY VGAPGAQYTC VHLYFLPEAF 

       250        260        270        280        290        300 
ETEDPRIFML EHYGVYDFYE ANGSGFDLVG PELVSSDGEA AGTPDADSSP PVMLPFERRI 

       310        320        330        340        350        360 
IPYNLRPLPS RSFTSDSFPA ARYSPAKTNS PPPSPASAAP ASAAPASAAP ASAAPASAAQ 

       370        380        390        400        410        420 
ASVAPASVAP ASAAPASAAP DSAAPASSPP LFIPIPGLGH TPGVPAPSTP PRASGSAAPQ 

       430        440        450        460        470        480 
TPKRKKGLGK DSPHKKPTSG RRLPLSSTTD TEDDQLPRPP VPPHRPPSAA RLPPPVIPIP 

       490        500        510        520        530        540 
HQSPPASPTP RRAPVSTIAP SVTPSPRLPL QIPIPLPQAA PSNPEIPLTT PSPSPTAAAA 

       550        560        570        580        590        600 
PTATTLSPPP TQQQPLQSAA PAPSPPPTQQ QPPQSAAPAP SPLLPQQQPP PSAAPAPSPL 

       610        620        630        640        650        660 
LPQQQPPPSA ARAPSPLPPQ QQPLPSATPA PPPAQQLPPS ATTLEPEKNN PSAADRAGTE 

       670        680        690        700        710        720 
ISPSPPFGQQ PSFGDDASGG SGLVRYLSDL EEPFLSMSDS EEAESDLASD IPTTEDEDMF 

       730        740        750        760        770        780 
EDEVFSNSLE SGSSAPTSPI TLDTARSQYY QTTFDIETPE MDFVPLESNI ARIAGHTYQE 

       790        800        810        820        830        840 
QAIVYDPASN REVPEADALS MIDYLLVTVV LEQGLIRSRD RSAVLNLLEF LKDWSGHLQV 

       850        860        870        880        890        900 
PTLDLEQLLT SELNIQNLAN MLSENKGRAG EFHEHLAAKL EACLPSLATK DAVRVDAGAK 

       910        920        930        940        950        960 
MLAEIPQLAE SDDGKFDLEA ARRRLTDLLS GGDQEGEEGG GEPEDHSIYR GPHVDVPLVL 

       970        980        990       1000       1010       1020 
DDESWKRLLS LAEAARTAVA RQQAGVDEED VRFLALLTAI EYGAPPAASV PPFVHNVAVR 

      1030       1040       1050       1060       1070       1080 
SKNAALHVRR CTADIRDKVA SAASDYLSYL EDPSLPTVMD FDDLLTHLRH TCQIIASLPL 

      1090       1100       1110       1120       1130       1140 
LNIRYTSIEW DYRELLYLGT ALSDMSGIPW PLERVEEDDP SIAPLPEFET VAKKQKELET 

      1150       1160       1170       1180       1190       1200 
TRENEKRLRT ILDDIEAMLG LAGVASAPGA PISPASPSAT PANHDNPEAT PPLADTAALT 

      1210       1220       1230       1240       1250       1260 
IPVIEKYIAN AGSIVGAAKN PTYIRLRDTI QQIVRSKKYL MNILKSITFY TIDNYIASFE 

      1270       1280       1290       1300       1310       1320 
ESIDHLYRDL PVLDPEVQDG IDRILDPMVS EALHTFEMGN RLTLEPARLV ALQNFATHST 

      1330       1340       1350       1360       1370       1380 
LKETAAAVNL LPGLLAVYDA TVTGQAPEDA LRLLSGLQNQ LSQTLIPGKL KKRFLSYLQK 

      1390       1400       1410       1420       1430       1440 
LKNNNNDQLR QKEVQAWRLE AEGFKPATEE QLEAFLDTAP NKELKRQYEK KLRQLMETGR 

      1450       1460       1470       1480       1490       1500 
KEKEKLREQE DKERRERRAR EANEAWARIR KALGARPEPA PTSPDDWNTL LASLLPDNTD 

      1510       1520       1530       1540       1550       1560 
SAAAAAAAVA RNTDILDSLT QILAAMLLGI TRVRRERLRS LLVDDGGAAE RMEAAEPGWF 

      1570       1580       1590       1600       1610       1620 
TDIETGPLAR LDAWPATPAA TAKEGGGGRG AEEAAGALFR ARTAADAIRS ALAQTRQALQ 

      1630       1640       1650       1660       1670       1680 
SPDMKSAVVN TDLEAPYAEY ERGLAGLLEK RRAAEAALTA IVSEYVDRTL PEATNDPGQA 

      1690       1700       1710       1720       1730       1740 
NLPPPPTIPQ ATAPPRLASD LALWPKKPQL LTRRERDDLL QATGDFFSEL LTEAEAAEVR 

      1750       1760       1770       1780       1790       1800 
ALEEQVRESQ TLMAKAHEMA ASTRRGFHTA LEAVLSRSRD EAPDDELRSL LPSPPKAPVQ 

      1810       1820       1830       1840       1850       1860 
APLEAALARA AAGNGSWPYR KSLAAAKWIR GICEAVRGLS EGALALAGGA GAWLNLAAAA 

      1870       1880       1890       1900       1910       1920 
DGEIHELTRL LEVEGMAQNS MDGMEELRLA LATLDPKRVA GGKETVADWK RRLSRLEAII 

      1930       1940       1950       1960       1970       1980 
QEAQEESQLQ GTLQDLVTQA RGHTDPRQLK IVVEAARGLA LGASAGSQYA LLKDKLLRYA 

      1990       2000       2010       2020       2030       2040 
SAKQSFLAFY ETAQPTVFVK HPLTNNLPLL ITISAPPTGW GNGAPTRRAQ FLAAAGPAKY 

      2050       2060       2070       2080       2090       2100 
AGTLWLETES PCDPLNPAYV SADTQEPLNY IPVYHNFLEY VMPTVLENPE AFSLTPAGRP 

      2110       2120       2130       2140       2150       2160 
QAIGPPQDDQ ERRRRTLASV ASARLSAAAA DSYWDTWPDV ESNAGELLRE YVSAPKALME 

      2170       2180       2190       2200       2210       2220 
DLADNPIVAM TLLAHASLIA SRNHPPYPAP ATDREVILLE QREIMALLVG THPAYAAAFL 

      2230       2240       2250       2260       2270       2280 
GAPSFYAGLG LVSALARDGG LGDLLSDSVL TYRLVRSPAS GRGGMPSTTR GSDDGEDARR 

      2290       2300       2310       2320       2330       2340 
LTRHRIAGPP TGFIFFQDAW EEMDTRAALW PHPEFLGLVH NQSTARARAC MLLLARRCFA 

      2350       2360       2370       2380       2390       2400 
PEALQQLWHS LRPLEGPVAF QDYLRDFVKQ AYTRGEELPR AEGLEVPRET PSSYGTVTGR 

      2410       2420       2430       2440       2450       2460 
ALRNLMPYGT PITGPKRGSG DTIPVSVFEA AVAAAFLGRP LTLFVSSQYL FNLKTLGQVR 

      2470       2480       2490       2500       2510       2520 
VVAPLLYCDG HSEPFRSLVE TISLNFLQDL DGYSESFEPE MSIFARQAVW LRELLTEARA 

      2530       2540       2550       2560       2570       2580 
AKPKEARPPT VAILANRKNI IWKCFTYRHN LPDVQFYFNA AGASRWPTDV LNPSFYEHED 

      2590       2600       2610       2620       2630       2640 
PPLPVGYQLP PNPRNVQELF SGFPPRVGHG LVSGDGFQSA DNTPASSDRL QQLGGGETDQ 

      2650       2660       2670       2680       2690       2700 
GEEGSTTAES EASGPPSPQS PLLEKVAPGR PRDWLSPTSS PRDVTVTPGL AAPITLPGPR 

      2710       2720       2730       2740       2750       2760 
LMARPYFGAE TRASESPDRS PGTSPRPWPK DSLELLPQPA PQQPPSSPWA SEQGPIVYTL 

      2770       2780       2790       2800       2810       2820 
SPHSTPSTAS GSQKKHTIQI PGLVPSQKPS YPPSAPYKPG QSTGGIAPTP SAASLTTFGL 

      2830       2840       2850       2860       2870       2880 
QPQDTQASSQ DPPYGHSIMQ REKKQQGGRE EAAEIRPSAT RLPTAVGLRP RAPVVAAGAA 

      2890       2900       2910       2920       2930       2940 
ASATPAFDPG EAPSGLPIPQ APALGSGLAA PAHTPVGALA PSPQKTQAQR PQDAAALPTP 

      2950       2960       2970       2980       2990       3000 
TIKAVGARPV PKATGALAAG ARPRGQPTAA PPSAASPPRV SLPVRSRQQQ SPAIPLPPMH 

      3010       3020       3030       3040       3050       3060 
SGSEPGARPE VRLSQYRHAG PQTYTVQKEA PPSAASQLPK MPKCKDSMYY PPSGSARYPA 

      3070       3080       3090       3100       3110       3120 
PFQALSFSQS VASPAPSSDQ TTLLWNTPSV VTQFLSIEDI IREVVTGGST SGDLVVPSGS 

      3130       3140       3150       3160       3170 
PSSLSTAAPE QDLRYSLTLS QASRVLSRFV SQLRRKLERS THRLIADLER LKFLYL 

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References

[1]"Genomic sequence analysis of Epstein-Barr virus strain GD1 from a nasopharyngeal carcinoma patient."
Zeng M.-S., Li D.-J., Liu Q.-L., Song L.-B., Li M.-Z., Zhang R.-H., Yu X.-J., Wang H.-M., Ernberg I., Zeng Y.-X.
J. Virol. 79:15323-15330(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY961628 Genomic DNA. Translation: AAY41102.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ3KSU8. 14 interactions.

Proteomic databases

PRIDEQ3KSU8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDEN_EBVG
AccessionPrimary (citable) accession number: Q3KSU8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 8, 2005
Last modified: June 26, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries