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Q3KSU5

- RIR1_EBVG

UniProt

Q3KSU5 - RIR1_EBVG

Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

BORF2

Organism
Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei171 – 1711SubstrateBy similarity
    Sitei187 – 1871Important for hydrogen atom transferBy similarity
    Binding sitei217 – 2171Substrate; via amide nitrogenBy similarity
    Active sitei387 – 3871Proton acceptorBy similarity
    Active sitei389 – 3891Cysteine radical intermediateBy similarity
    Active sitei391 – 3911Proton acceptorBy similarity
    Sitei403 – 4031Important for hydrogen atom transferBy similarity
    Sitei725 – 7251Important for electron transferBy similarity
    Sitei726 – 7261Important for electron transferBy similarity
    Sitei822 – 8221Interacts with thioredoxin/glutaredoxinBy similarity
    Sitei825 – 8251Interacts with thioredoxin/glutaredoxinBy similarity

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase 140 kDa subunit
    Ribonucleotide reductase large subunit
    Gene namesi
    ORF Names:BORF2
    OrganismiEpstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
    Taxonomic identifieri10376 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000007641: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 826826Ribonucleoside-diphosphate reductase large subunitPRO_0000375966Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi187 ↔ 403Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Subunit structurei

    Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit BaRF1 (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ3KSU5. 2 interactions.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni186 – 1872Substrate bindingBy similarity
    Regioni387 – 3915Substrate bindingBy similarity
    Regioni594 – 5985Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    [Graphical view]
    PfamiPF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
    PROSITEiPS00089. RIBORED_LARGE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3KSU5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATTNHVEHE LLSKLIDELK VKANADPEAD VLAGRLLHRL KAESVTHTVA    50
    EYLEVFSDKF YDEEFFQMHR DELETRVSAF AQSPAYERIV SSGYLSALRY 100
    YDTYLYVGRS GKQESVQHFY MRLAGFCAST TCLYAGLRAA LQRARPEIES 150
    DMEVFDYYFE HLTSQTVCCS TPFMRFAGVE NSTLASCILT TPDLSSEWDV 200
    TQALYRHLGR YLFQRAGVGV GVTGAGQDGK HISLLMRMIN SHVEYHNYGC 250
    KRPVSVAAYM EPWHSQIFKF LETKLPENHE RCPGIFTGLF VPELFFKLFR 300
    DTPWSDWYLF DPKDAGDLER LYGEEFEREY YRLVTAGKFC GRVSIKSLMF 350
    SIVNCAVKAG SPFILLKEAC NAHFWRDLQG EAMNAANLCA EVLQPSRKSV 400
    ATCNLANICL PRCLVNAPLA VRAQRADTQG DELLLALPRL SVTLPGEGAI 450
    GDGFSLARLR DATQCATFVV ACSILQGSPT YDSRDMASMG LGVQGLADVF 500
    ADLGWQYTDP PSRSLNKEIF EHMYFTALCT SSLIGLHTRK IFPGFKQSKY 550
    AGGWFHWHDW AGTDLSIPRE IWSRLSERIV RDGLFNSQFI ALMPTSGCAQ 600
    VTGCSDAFYP FYANASTKVT NKEEALRPNR SFWRHVRLDD REALNLVGGR 650
    VSCLPEALRQ RYLRFQTAFD YNQEDLIQMS RDRAPFVDQS QSHSLFLREE 700
    DAARASTLAN LLVRSYELGL KTIMYYCRIE KAADLGVMEC KASAALSVPR 750
    EEQNERSPAE QMLPRPMEPA QVTGPVDIMS KGPGEGPGGW CVPGGLEVCY 800
    KYRQLFSEDD LLETDGFTER ACESCQ 826
    Length:826
    Mass (Da):93,102
    Last modified:November 8, 2005 - v1
    Checksum:iEC04EBDD0A298389
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY961628 Genomic DNA. Translation: AAY41105.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY961628 Genomic DNA. Translation: AAY41105.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q3KSU5. 2 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    InterProi IPR013346. NrdE_NrdA.
    IPR000788. RNR_lg_C.
    IPR013509. RNR_lsu_N.
    [Graphical view ]
    Pfami PF02867. Ribonuc_red_lgC. 1 hit.
    PF00317. Ribonuc_red_lgN. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
    PROSITEi PS00089. RIBORED_LARGE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequence analysis of Epstein-Barr virus strain GD1 from a nasopharyngeal carcinoma patient."
      Zeng M.-S., Li D.-J., Liu Q.-L., Song L.-B., Li M.-Z., Zhang R.-H., Yu X.-J., Wang H.-M., Ernberg I., Zeng Y.-X.
      J. Virol. 79:15323-15330(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Tinkering with a viral ribonucleotide reductase."
      Lembo D., Brune W.
      Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiRIR1_EBVG
    AccessioniPrimary (citable) accession number: Q3KSU5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 26, 2009
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3