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Q3KSU5

- RIR1_EBVG

UniProt

Q3KSU5 - RIR1_EBVG

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Protein
Ribonucleoside-diphosphate reductase large subunit
Gene
BORF2
Organism
Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei171 – 1711Substrate By similarity
Sitei187 – 1871Important for hydrogen atom transfer By similarity
Binding sitei217 – 2171Substrate; via amide nitrogen By similarity
Active sitei387 – 3871Proton acceptor By similarity
Active sitei389 – 3891Cysteine radical intermediate By similarity
Active sitei391 – 3911Proton acceptor By similarity
Sitei403 – 4031Important for hydrogen atom transfer By similarity
Sitei725 – 7251Important for electron transfer By similarity
Sitei726 – 7261Important for electron transfer By similarity
Sitei822 – 8221Interacts with thioredoxin/glutaredoxin By similarity
Sitei825 – 8251Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase 140 kDa subunit
Ribonucleotide reductase large subunit
Gene namesi
ORF Names:BORF2
OrganismiEpstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10376 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007641: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 826826Ribonucleoside-diphosphate reductase large subunit
PRO_0000375966Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi187 ↔ 403Redox-active By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit BaRF1 (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Protein-protein interaction databases

IntActiQ3KSU5. 2 interactions.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni186 – 1872Substrate binding By similarity
Regioni387 – 3915Substrate binding By similarity
Regioni594 – 5985Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3KSU5-1 [UniParc]FASTAAdd to Basket

« Hide

MATTNHVEHE LLSKLIDELK VKANADPEAD VLAGRLLHRL KAESVTHTVA    50
EYLEVFSDKF YDEEFFQMHR DELETRVSAF AQSPAYERIV SSGYLSALRY 100
YDTYLYVGRS GKQESVQHFY MRLAGFCAST TCLYAGLRAA LQRARPEIES 150
DMEVFDYYFE HLTSQTVCCS TPFMRFAGVE NSTLASCILT TPDLSSEWDV 200
TQALYRHLGR YLFQRAGVGV GVTGAGQDGK HISLLMRMIN SHVEYHNYGC 250
KRPVSVAAYM EPWHSQIFKF LETKLPENHE RCPGIFTGLF VPELFFKLFR 300
DTPWSDWYLF DPKDAGDLER LYGEEFEREY YRLVTAGKFC GRVSIKSLMF 350
SIVNCAVKAG SPFILLKEAC NAHFWRDLQG EAMNAANLCA EVLQPSRKSV 400
ATCNLANICL PRCLVNAPLA VRAQRADTQG DELLLALPRL SVTLPGEGAI 450
GDGFSLARLR DATQCATFVV ACSILQGSPT YDSRDMASMG LGVQGLADVF 500
ADLGWQYTDP PSRSLNKEIF EHMYFTALCT SSLIGLHTRK IFPGFKQSKY 550
AGGWFHWHDW AGTDLSIPRE IWSRLSERIV RDGLFNSQFI ALMPTSGCAQ 600
VTGCSDAFYP FYANASTKVT NKEEALRPNR SFWRHVRLDD REALNLVGGR 650
VSCLPEALRQ RYLRFQTAFD YNQEDLIQMS RDRAPFVDQS QSHSLFLREE 700
DAARASTLAN LLVRSYELGL KTIMYYCRIE KAADLGVMEC KASAALSVPR 750
EEQNERSPAE QMLPRPMEPA QVTGPVDIMS KGPGEGPGGW CVPGGLEVCY 800
KYRQLFSEDD LLETDGFTER ACESCQ 826
Length:826
Mass (Da):93,102
Last modified:November 8, 2005 - v1
Checksum:iEC04EBDD0A298389
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY961628 Genomic DNA. Translation: AAY41105.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY961628 Genomic DNA. Translation: AAY41105.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q3KSU5. 2 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic sequence analysis of Epstein-Barr virus strain GD1 from a nasopharyngeal carcinoma patient."
    Zeng M.-S., Li D.-J., Liu Q.-L., Song L.-B., Li M.-Z., Zhang R.-H., Yu X.-J., Wang H.-M., Ernberg I., Zeng Y.-X.
    J. Virol. 79:15323-15330(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Tinkering with a viral ribonucleotide reductase."
    Lembo D., Brune W.
    Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiRIR1_EBVG
AccessioniPrimary (citable) accession number: Q3KSU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 8, 2005
Last modified: October 16, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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