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Q3KSQ2

- KITH_EBVG

UniProt

Q3KSQ2 - KITH_EBVG

Protein

Thymidine kinase

Gene

TK

Organism
Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
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    Functioni

    In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as memory B-cells that do not replicate their genome. Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during DNA replication By similarity.By similarity

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei317 – 3171Proton acceptorSequence Analysis
    Binding sitei355 – 3551SubstrateBy similarity
    Binding sitei402 – 4021SubstrateBy similarity
    Binding sitei445 – 4451ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi291 – 2988ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. thymidine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. TMP biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Gene namesi
    Name:TK
    ORF Names:BXLF1
    OrganismiEpstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
    Taxonomic identifieri10376 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000007641: Genome

    Subcellular locationi

    Virion tegument. Host nucleus By similarity
    Note: Localizes to the centrosome and more precisely to the periphery of the centriole, tightly encircling the tubulin-rich centrioles.By similarity

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell
    2. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 607607Thymidine kinasePRO_0000375958Add
    BLAST

    Expressioni

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BGLF4P0C7312EBI-2621028,EBI-2621032

    Protein-protein interaction databases

    IntActiQ3KSQ2. 8 interactions.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR001889. Herpes_TK.
    IPR013672. Herpes_TK_C.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00693. Herpes_TK. 1 hit.
    PF08465. Herpes_TK_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q3KSQ2-1 [UniParc]FASTAAdd to Basket

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    MAGFPGKEAG PPGGWRKCQE DESPENERHE NFYAEIDDFA PSVLTPTGSD    50
    SGAGEEDDDG LYQVPTHWPP LMAPTGLSGE RVPCRTQAAV TSNTGNSPGS 100
    RHTSCPFTLP RGAQPPAPAH QKPTAPTPKP RSRECGPSKT PDPFSWFRKT 150
    SCTEGGADST SRSFMYQKGF EEGLAGLGLD DKSDCESEDE SNFRRPSSHS 200
    ALKQKNGGKG KPSGLFEHLA AHGREFSKLS KHAAQLKRLS GSVMNVLNLD 250
    DAQDTRQAKA QRKESTRVPI VIHLTNHVPV IKPACSLFLE GAPGVGKTTM 300
    LNHLKAVFGD LTIVVPEPMR YWTHVYENAI KAMHKNVTRA RHGREDTSAE 350
    VLACQMKFTT PFRVLASRKR SLLVTESGAR SVAPLDCWIL HDRHLLSASV 400
    VFPLMLLRSQ LLSYSDFIQV LATFTADPGD TIVWMKLNVE ENMRRLKKRG 450
    RKHESGLDAG YLKSVNDAYH AVYCAWLLTQ YFAPEDIVKV CAGLTTITTV 500
    CHQSHTPIIR SGVAEKLYKN SIYSVLKEVI QPYRADAVLL EVCLAFTRTL 550
    AYLQFVLVDL SEFQDDLPGC WTEIYMQALK NPAIRSQFFD WAGLSKVISD 600
    FERGNRD 607
    Length:607
    Mass (Da):67,207
    Last modified:November 8, 2005 - v1
    Checksum:i163836CEDA39C52C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY961628 Genomic DNA. Translation: AAY41147.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY961628 Genomic DNA. Translation: AAY41147.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q3KSQ2. 8 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR001889. Herpes_TK.
    IPR013672. Herpes_TK_C.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00693. Herpes_TK. 1 hit.
    PF08465. Herpes_TK_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequence analysis of Epstein-Barr virus strain GD1 from a nasopharyngeal carcinoma patient."
      Zeng M.-S., Li D.-J., Liu Q.-L., Song L.-B., Li M.-Z., Zhang R.-H., Yu X.-J., Wang H.-M., Ernberg I., Zeng Y.-X.
      J. Virol. 79:15323-15330(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiKITH_EBVG
    AccessioniPrimary (citable) accession number: Q3KSQ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 26, 2009
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Phosphorylates and thereby activates certain drugs like acyclovir (ACV), valacyclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK.By similarity

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3