Q3KSQ2 (KITH_EBVG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	TK
ORF Names:	BXLF1

Organism

Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4) [Complete proteome]

Taxonomic identifier

10376 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Gammaherpesvirinae › Lymphocryptovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	607 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as memory B-cells that do not replicate their genome. Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during DNA replication By similarity.
Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Virion tegument. Host nucleus By similarity. Note: Localizes to the centrosome and more precisely to the periphery of the centriole, tightly encircling the tubulin-rich centrioles By similarity.
Miscellaneous	Phosphorylates and thereby activates certain drugs like acyclovir (ACV), valacyclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK By similarity.
Sequence similarities	Belongs to the herpesviruses thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Host nucleus Virion Virion tegument
Developmental stage	Early protein
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW TMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell viral tegument Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BGLF4	P0C731	2	EBI-2621028,EBI-2621032

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 607	607	Thymidine kinase		PRO_0000375958
Regions
	Nucleotide binding	291 – 298	8	ATP By similarity
Sites
	Active site	317	1	Proton acceptor Potential
	Binding site	355	1	Substrate By similarity
	Binding site	402	1	Substrate By similarity
	Binding site	445	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3KSQ2 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 163836CEDA39C52C	FASTA	607	67,207
	10 20 30 40 50 60 MAGFPGKEAG PPGGWRKCQE DESPENERHE NFYAEIDDFA PSVLTPTGSD SGAGEEDDDG 70 80 90 100 110 120 LYQVPTHWPP LMAPTGLSGE RVPCRTQAAV TSNTGNSPGS RHTSCPFTLP RGAQPPAPAH 130 140 150 160 170 180 QKPTAPTPKP RSRECGPSKT PDPFSWFRKT SCTEGGADST SRSFMYQKGF EEGLAGLGLD 190 200 210 220 230 240 DKSDCESEDE SNFRRPSSHS ALKQKNGGKG KPSGLFEHLA AHGREFSKLS KHAAQLKRLS 250 260 270 280 290 300 GSVMNVLNLD DAQDTRQAKA QRKESTRVPI VIHLTNHVPV IKPACSLFLE GAPGVGKTTM 310 320 330 340 350 360 LNHLKAVFGD LTIVVPEPMR YWTHVYENAI KAMHKNVTRA RHGREDTSAE VLACQMKFTT 370 380 390 400 410 420 PFRVLASRKR SLLVTESGAR SVAPLDCWIL HDRHLLSASV VFPLMLLRSQ LLSYSDFIQV 430 440 450 460 470 480 LATFTADPGD TIVWMKLNVE ENMRRLKKRG RKHESGLDAG YLKSVNDAYH AVYCAWLLTQ 490 500 510 520 530 540 YFAPEDIVKV CAGLTTITTV CHQSHTPIIR SGVAEKLYKN SIYSVLKEVI QPYRADAVLL 550 560 570 580 590 600 EVCLAFTRTL AYLQFVLVDL SEFQDDLPGC WTEIYMQALK NPAIRSQFFD WAGLSKVISD FERGNRD

References

[1]

"Genomic sequence analysis of Epstein-Barr virus strain GD1 from a nasopharyngeal carcinoma patient." Zeng M.-S., Li D.-J., Liu Q.-L., Song L.-B., Li M.-Z., Zhang R.-H., Yu X.-J., Wang H.-M., Ernberg I., Zeng Y.-X. J. Virol. 79:15323-15330(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY961628 Genomic DNA. Translation: AAY41147.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q3KSQ2. 8 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001889. Herpes_TK. IPR013672. Herpes_TK_C. [Graphical view]
Pfam	PF00693. Herpes_TK. 1 hit. PF08465. Herpes_TK_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_EBVG

Accession

Primary (citable) accession number: Q3KSQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	November 8, 2005
Last modified:	July 11, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program