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Q3KSQ2

- KITH_EBVG

UniProt

Q3KSQ2 - KITH_EBVG

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Protein

Thymidine kinase

Gene

TK

Organism
Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as memory B-cells that do not replicate their genome. Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during DNA replication (By similarity).By similarity

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei317 – 3171Proton acceptorSequence Analysis
Binding sitei355 – 3551SubstrateBy similarity
Binding sitei402 – 4021SubstrateBy similarity
Binding sitei445 – 4451ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi291 – 2988ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. thymidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. TMP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
ORF Names:BXLF1
OrganismiEpstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10376 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007641: Genome

Subcellular locationi

Virion tegument. Host nucleus By similarity
Note: Localizes to the centrosome and more precisely to the periphery of the centriole, tightly encircling the tubulin-rich centrioles.By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
  2. viral tegument Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 607607Thymidine kinasePRO_0000375958Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BGLF4P0C7312EBI-2621028,EBI-2621032

Protein-protein interaction databases

IntActiQ3KSQ2. 8 interactions.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR013672. Herpes_TK_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
PF08465. Herpes_TK_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3KSQ2 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGFPGKEAG PPGGWRKCQE DESPENERHE NFYAEIDDFA PSVLTPTGSD
60 70 80 90 100
SGAGEEDDDG LYQVPTHWPP LMAPTGLSGE RVPCRTQAAV TSNTGNSPGS
110 120 130 140 150
RHTSCPFTLP RGAQPPAPAH QKPTAPTPKP RSRECGPSKT PDPFSWFRKT
160 170 180 190 200
SCTEGGADST SRSFMYQKGF EEGLAGLGLD DKSDCESEDE SNFRRPSSHS
210 220 230 240 250
ALKQKNGGKG KPSGLFEHLA AHGREFSKLS KHAAQLKRLS GSVMNVLNLD
260 270 280 290 300
DAQDTRQAKA QRKESTRVPI VIHLTNHVPV IKPACSLFLE GAPGVGKTTM
310 320 330 340 350
LNHLKAVFGD LTIVVPEPMR YWTHVYENAI KAMHKNVTRA RHGREDTSAE
360 370 380 390 400
VLACQMKFTT PFRVLASRKR SLLVTESGAR SVAPLDCWIL HDRHLLSASV
410 420 430 440 450
VFPLMLLRSQ LLSYSDFIQV LATFTADPGD TIVWMKLNVE ENMRRLKKRG
460 470 480 490 500
RKHESGLDAG YLKSVNDAYH AVYCAWLLTQ YFAPEDIVKV CAGLTTITTV
510 520 530 540 550
CHQSHTPIIR SGVAEKLYKN SIYSVLKEVI QPYRADAVLL EVCLAFTRTL
560 570 580 590 600
AYLQFVLVDL SEFQDDLPGC WTEIYMQALK NPAIRSQFFD WAGLSKVISD

FERGNRD
Length:607
Mass (Da):67,207
Last modified:November 8, 2005 - v1
Checksum:i163836CEDA39C52C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY961628 Genomic DNA. Translation: AAY41147.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY961628 Genomic DNA. Translation: AAY41147.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q3KSQ2. 8 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR001889. Herpes_TK.
IPR013672. Herpes_TK_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00693. Herpes_TK. 1 hit.
PF08465. Herpes_TK_C. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genomic sequence analysis of Epstein-Barr virus strain GD1 from a nasopharyngeal carcinoma patient."
    Zeng M.-S., Li D.-J., Liu Q.-L., Song L.-B., Li M.-Z., Zhang R.-H., Yu X.-J., Wang H.-M., Ernberg I., Zeng Y.-X.
    J. Virol. 79:15323-15330(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiKITH_EBVG
AccessioniPrimary (citable) accession number: Q3KSQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 8, 2005
Last modified: October 29, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Phosphorylates and thereby activates certain drugs like acyclovir (ACV), valacyclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK.By similarity

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3