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Protein

Eukaryotic translation initiation factor 6

Gene

Eif6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis. Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis, Ribosome biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 6UniRule annotation
Short name:
eIF-6UniRule annotation
Gene namesi
Name:Eif6
Synonyms:Itgb4bp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1305373 Eif6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004020951 – 245Eukaryotic translation initiation factor 6Add BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei113PhosphotyrosineBy similarity1
Modified residuei165PhosphothreonineUniRule annotationBy similarity1
Modified residuei166PhosphoserineUniRule annotationBy similarity1
Modified residuei174Phosphoserine; by CK1UniRule annotationBy similarity1
Modified residuei175Phosphoserine; by CK1UniRule annotationBy similarity1
Modified residuei235Phosphoserine; by PKCUniRule annotationBy similarity1
Modified residuei239PhosphoserineUniRule annotationBy similarity1
Modified residuei243PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-174 and Ser-175 by CSNK1D/CK1 promotes nuclear export.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3KRD8
PRIDEiQ3KRD8

PTM databases

iPTMnetiQ3KRD8
PhosphoSitePlusiQ3KRD8

Expressioni

Gene expression databases

BgeeiENSRNOG00000049497
ExpressionAtlasiQ3KRD8 baseline and differential
GenevisibleiQ3KRD8 RN

Interactioni

Subunit structurei

Monomer. Associates with the 60S ribosomal subunit. Interacts with RACK1. Interacts with DICER1, AGO2, TARBP2, MOV10 and RPL7A; they form a large RNA-induced silencing complex (RISC).UniRule annotation

Protein-protein interaction databases

BioGridi258203, 1 interactor
STRINGi10116.ENSRNOP00000066257

Structurei

3D structure databases

ProteinModelPortaliQ3KRD8
SMRiQ3KRD8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-6 family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3185 Eukaryota
COG1976 LUCA
GeneTreeiENSGT00390000015972
HOVERGENiHBG001300
InParanoidiQ3KRD8
KOiK03264
OMAiNDWCAFT
PhylomeDBiQ3KRD8

Family and domain databases

CDDicd00527 IF6, 1 hit
HAMAPiMF_00032 eIF_6, 1 hit
InterProiView protein in InterPro
IPR002769 eIF6
PANTHERiPTHR10784 PTHR10784, 1 hit
PfamiView protein in Pfam
PF01912 eIF-6, 1 hit
PIRSFiPIRSF006413 IF-6, 1 hit
SMARTiView protein in SMART
SM00654 eIF6, 1 hit
TIGRFAMsiTIGR00323 eIF-6, 1 hit

Sequencei

Sequence statusi: Complete.

Q3KRD8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVRASFENN CEVGCFAKLT NTYCLVAIGG SENFYSVFEG ELSDTIPVVH
60 70 80 90 100
ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLPD SVQIRRVEER
110 120 130 140 150
LSALGNVTTC NDYVALVHPD LDRETEEILA DVLKVEVFRQ TVADQVLVGS
160 170 180 190 200
YCVFSNQGGL VHPKTSIEDQ DELSSLLQVP LVAGTVNRGS EVIAAGMVVN
210 220 230 240
DWCAFCGLDT TSTELSVVES VFKLNEAKPS TIATSMRDSL IDSLT
Length:245
Mass (Da):26,571
Last modified:November 8, 2005 - v1
Checksum:i046FA8F06709F69A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474050 Genomic DNA Translation: EDL85894.1
BC105764 mRNA Translation: AAI05765.1
RefSeqiNP_001032429.1, NM_001037352.1
XP_006235378.1, XM_006235316.3
XP_017447156.1, XM_017591667.1
UniGeneiRn.190833

Genome annotation databases

EnsembliENSRNOT00000073492; ENSRNOP00000066257; ENSRNOG00000049497
GeneIDi305506
KEGGirno:305506

Similar proteinsi

Entry informationi

Entry nameiIF6_RAT
AccessioniPrimary (citable) accession number: Q3KRD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: November 8, 2005
Last modified: March 28, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health