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Protein

Aspartate--tRNA ligase, mitochondrial

Gene

Dars2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei265 – 2651AspartateBy similarity
Binding sitei534 – 5341ATPBy similarity
Binding sitei541 – 5411AspartateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi265 – 2673ATPBy similarity
Nucleotide bindingi583 – 5864ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligase, mitochondrial (EC:6.1.1.12)
Alternative name(s):
Aspartyl-tRNA synthetase
Short name:
AspRS
Gene namesi
Name:Dars2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1308286. Dars2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4646MitochondrionSequence analysisAdd
BLAST
Chaini47 – 652606Aspartate--tRNA ligase, mitochondrialPRO_0000250738Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei218 – 2181PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3KRD0.
PRIDEiQ3KRD0.

PTM databases

iPTMnetiQ3KRD0.
PhosphoSiteiQ3KRD0.

Expressioni

Gene expression databases

GenevisibleiQ3KRD0. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003828.

Structurei

3D structure databases

ProteinModelPortaliQ3KRD0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni243 – 2464AspartateBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2411. Eukaryota.
COG0173. LUCA.
GeneTreeiENSGT00550000074971.
HOGENOMiHOG000275159.
HOVERGENiHBG055815.
InParanoidiQ3KRD0.
KOiK01876.
OMAiYQLDVEM.
OrthoDBiEOG773XFN.
PhylomeDBiQ3KRD0.
TreeFamiTF314827.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_bac/mit.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 2 hits.
PTHR22594:SF5. PTHR22594:SF5. 2 hits.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3KRD0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYLGSWLNRL GRGLSRPIGK TKQPIWGSLS RSLTLSSQRV PEFSSFVART
60 70 80 90 100
NTCGELRSSH LGQEVTLCGW IQYRRQNTFL VLRDCHGLVQ ILIPQDESAA
110 120 130 140 150
SVRRTLCEAP VESVVRVSGT VIARPLGQEN PKMPTGEIEI KAKTAELLNA
160 170 180 190 200
CKKLPFEIKD FVKKTEALRL QYRYLDLRSS QMQHNLRLRS QMVMKMREYL
210 220 230 240 250
CTLHGFVDIE TPTLFKRTPG GAKEFLVPSR EPGRFYSLPQ SPQQFKQLLM
260 270 280 290 300
VGGLDRYFQV ARCYRDEGSR PDRQPEFTQI DIEMSFVDQT GIQHLVEGLL
310 320 330 340 350
HYSWPEDKDP LVAPFPSMTF AEALATYGTD KPDTRFGMKI VDISDVFRNT
360 370 380 390 400
EIRFLQDALA KPQGTVKAIC VHEGAKYLRK EDIEFIRKFA AHHFSQEVLP
410 420 430 440 450
IFLNARKNWS SPFAKFITEE ERLELTRLME IQEDDMVLLT AGQHEKACSL
460 470 480 490 500
LGKLRLECAD LLETRGLALR DPALFSFLWV LDFPLFLAKE ESPTELESAH
510 520 530 540 550
HPFTAPHPGD IHLLYTEPEK VRGQHYDLVL NGNEIGGGSI RIHDAQLQRY
560 570 580 590 600
ILETLLKEDV KLLSHLLQAL DYGAPPHGGI ALGLDRLVCL VTGAPSIRDV
610 620 630 640 650
IAFPKSYRGH DLMSNAPDTV SPEDLKPYHI HVSWPTDSEE RASATPSKYL

SS
Length:652
Mass (Da):73,953
Last modified:November 8, 2005 - v1
Checksum:i6CB570CC05F30086
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105774 mRNA. Translation: AAI05775.1.
RefSeqiNP_001029315.1. NM_001034143.1.
UniGeneiRn.137838.

Genome annotation databases

EnsembliENSRNOT00000003828; ENSRNOP00000003828; ENSRNOG00000002813.
GeneIDi304919.
KEGGirno:304919.
UCSCiRGD:1308286. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105774 mRNA. Translation: AAI05775.1.
RefSeqiNP_001029315.1. NM_001034143.1.
UniGeneiRn.137838.

3D structure databases

ProteinModelPortaliQ3KRD0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003828.

PTM databases

iPTMnetiQ3KRD0.
PhosphoSiteiQ3KRD0.

Proteomic databases

PaxDbiQ3KRD0.
PRIDEiQ3KRD0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003828; ENSRNOP00000003828; ENSRNOG00000002813.
GeneIDi304919.
KEGGirno:304919.
UCSCiRGD:1308286. rat.

Organism-specific databases

CTDi55157.
RGDi1308286. Dars2.

Phylogenomic databases

eggNOGiKOG2411. Eukaryota.
COG0173. LUCA.
GeneTreeiENSGT00550000074971.
HOGENOMiHOG000275159.
HOVERGENiHBG055815.
InParanoidiQ3KRD0.
KOiK01876.
OMAiYQLDVEM.
OrthoDBiEOG773XFN.
PhylomeDBiQ3KRD0.
TreeFamiTF314827.

Miscellaneous databases

PROiQ3KRD0.

Gene expression databases

GenevisibleiQ3KRD0. RN.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_bac/mit.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 2 hits.
PTHR22594:SF5. PTHR22594:SF5. 2 hits.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  2. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-218, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYDM_RAT
AccessioniPrimary (citable) accession number: Q3KRD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: November 8, 2005
Last modified: June 8, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.