Reviewed, UniProtKB/Swiss-Prot Q3KRD0 (SYDM_RAT)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase, mitochondrial
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:

Dars2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	652 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 46	46	Mitochondrion Potential
	Chain	47 – 652	606	Aspartyl-tRNA synthetase, mitochondrial		PRO_0000250738
Amino acid modifications
	Modified residue	626	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3KRD0-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 6CB570CC05F30086	FASTA	652	73,953
	10 20 30 40 50 60 MYLGSWLNRL GRGLSRPIGK TKQPIWGSLS RSLTLSSQRV PEFSSFVART NTCGELRSSH 70 80 90 100 110 120 LGQEVTLCGW IQYRRQNTFL VLRDCHGLVQ ILIPQDESAA SVRRTLCEAP VESVVRVSGT 130 140 150 160 170 180 VIARPLGQEN PKMPTGEIEI KAKTAELLNA CKKLPFEIKD FVKKTEALRL QYRYLDLRSS 190 200 210 220 230 240 QMQHNLRLRS QMVMKMREYL CTLHGFVDIE TPTLFKRTPG GAKEFLVPSR EPGRFYSLPQ 250 260 270 280 290 300 SPQQFKQLLM VGGLDRYFQV ARCYRDEGSR PDRQPEFTQI DIEMSFVDQT GIQHLVEGLL 310 320 330 340 350 360 HYSWPEDKDP LVAPFPSMTF AEALATYGTD KPDTRFGMKI VDISDVFRNT EIRFLQDALA 370 380 390 400 410 420 KPQGTVKAIC VHEGAKYLRK EDIEFIRKFA AHHFSQEVLP IFLNARKNWS SPFAKFITEE 430 440 450 460 470 480 ERLELTRLME IQEDDMVLLT AGQHEKACSL LGKLRLECAD LLETRGLALR DPALFSFLWV 490 500 510 520 530 540 LDFPLFLAKE ESPTELESAH HPFTAPHPGD IHLLYTEPEK VRGQHYDLVL NGNEIGGGSI 550 560 570 580 590 600 RIHDAQLQRY ILETLLKEDV KLLSHLLQAL DYGAPPHGGI ALGLDRLVCL VTGAPSIRDV 610 620 630 640 650 IAFPKSYRGH DLMSNAPDTV SPEDLKPYHI HVSWPTDSEE RASATPSKYL SS

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC105774 mRNA. Translation: AAI05775.1.
IPI	IPI00372374.
RefSeq	NP_001029315.1.
UniGene	Rn.137838
3D structure databases
SMR	Q3KRD0. Positions 48-635.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3KRD0.
Proteomic databases
PRIDE	Q3KRD0.
Genome annotation databases
Ensembl	ENSRNOT00000003828; ENSRNOP00000003828; ENSRNOG00000002813; Rattus norvegicus. [Genome view]
GeneID	304919.
KEGG	rno:304919.
NMPDR	fig|10116.3.peg.9359.
UCSC	NM_001034143. rat.
Organism-specific databases
CTD	304919.
RGD	1308286. Dars2.
Phylogenomic databases
eggNOG	roNOG07016.
HOVERGEN	Q3KRD0.
InParanoid	Q3KRD0.
OMA	PLFEYDE.
OrthoDB	EOG91ZHWP.
PhylomeDB	Q3KRD0.
Enzyme and pathway databases
BRENDA	6.1.1.12. 248.
Gene expression databases
ArrayExpress	Q3KRD0.
Genevestigator	Q3KRD0.
GermOnline	ENSRNOG00000002813. Rattus norvegicus.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR002312. Asp-tRNA-synth_IIb. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	653833.

Entry information

Entry name

SYDM_RAT

Accession

Primary (citable) accession number: Q3KRD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	November 8, 2005
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)