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Protein

Volume-regulated anion channel subunit LRRC8E

Gene

Lrrc8e

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and may also conduct organic osmolytes like taurine. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Volume-regulated anion channel subunit LRRC8E
Alternative name(s):
Leucine-rich repeat-containing protein 8E
Gene namesi
Name:Lrrc8e
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1311979. Lrrc8e.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Endoplasmic reticulum membrane By similarity

  • Note: In the absence of LRRC8A, resides primarily in a cytoplasmic compartment, probably the endoplasmic reticulum. Requires LRRC8A for expression at the cell membrane.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 43HelicalSequence analysisAdd BLAST21
Topological domaini44 – 116ExtracellularSequence analysisAdd BLAST73
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Topological domaini138 – 264CytoplasmicSequence analysisAdd BLAST127
Transmembranei265 – 285HelicalSequence analysisAdd BLAST21
Topological domaini286 – 312ExtracellularSequence analysisAdd BLAST27
Transmembranei313 – 333HelicalSequence analysisAdd BLAST21
Topological domaini334 – 795CytoplasmicSequence analysisAdd BLAST462

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000762521 – 795Volume-regulated anion channel subunit LRRC8EAdd BLAST795

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi63N-linked (GlcNAc...)Sequence analysis1
Glycosylationi301N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ3KRC6.
PRIDEiQ3KRC6.

PTM databases

PhosphoSitePlusiQ3KRC6.

Expressioni

Gene expression databases

BgeeiENSRNOG00000028460.
GenevisibleiQ3KRC6. RN.

Interactioni

Subunit structurei

Heterooligomer; heterooligomerizes with other LRRC8 proteins (LRRC8A, LRRC8C, LRRC8D and/or LRRC8B), possibly to form a heterohexamer. In vivo, the subunit composition may depend primarily on expression levels, and heterooligomeric channels containing various proportions of the different LRRC8 proteins may coexist.By similarity

Protein-protein interaction databases

BioGridi257792. 1 interactor.
STRINGi10116.ENSRNOP00000037487.

Structurei

3D structure databases

ProteinModelPortaliQ3KRC6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati535 – 556LRR 1Add BLAST22
Repeati558 – 578LRR 2Add BLAST21
Repeati582 – 603LRR 3Add BLAST22
Repeati605 – 626LRR 4Add BLAST22
Repeati630 – 651LRR 5Add BLAST22
Repeati653 – 674LRR 6Add BLAST22
Repeati676 – 697LRR 7Add BLAST22
Repeati699 – 720LRR 8Add BLAST22
Repeati722 – 743LRR 9Add BLAST22
Repeati745 – 766LRR 10Add BLAST22

Sequence similaritiesi

Belongs to the LRRC8 family.Curated
Contains 10 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00840000129676.
HOGENOMiHOG000231806.
HOVERGENiHBG052360.
InParanoidiQ3KRC6.
OMAiFINQLCY.
OrthoDBiEOG091G01ER.
PhylomeDBiQ3KRC6.
TreeFamiTF331443.

Family and domain databases

Gene3Di3.80.10.10. 3 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR021040. LRRC8_Pannexin-like.
[Graphical view]
PfamiPF13855. LRR_8. 2 hits.
PF12534. Pannexin_like. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 6 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 8 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3KRC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPVAEFKQF TEQQPAFKVL KPWWDVLAEY LTVAMLMIGV FGCTLQVTQD
60 70 80 90 100
KIICLPSHES RENSSEAPCQ QLLPQGISEQ IGGLRELSGL KNNLDLQQYS
110 120 130 140 150
FINQLCYETA LHWYAKYFPY LVVIHTLIFM VCTSFWFKFP GTSSKIEHFI
160 170 180 190 200
SILGKCFDSP WTTRALSEVS GENHKGPAAG RATVTTVTTV GTGTGKVGEG
210 220 230 240 250
EKEKVLIEPE KVVTEPPAVT LLDKKEGEQA KALFEKVKKF RVHVEEGDIL
260 270 280 290 300
YSMYIRQTVL KVCKFFAILV YNLVYVEKIS FLVACRVETS EITGYASFCC
310 320 330 340 350
NHTKAHLFSK LAFCYISFVC VYGITCLYTL YWLFHRPLKE YSFRSVREET
360 370 380 390 400
GMNDIPDVRN DFAFMLHLID QYDSLYSKRF AVFLSEVSES RLKQLNLNHE
410 420 430 440 450
WTPDKLRQKL QRNARGRLEL SLCMLPGLPD TVFELSEVEA LRLEAICDIS
460 470 480 490 500
FPPGLSQLVN LQELSLLHSP ARLPFSSQIF LRDRLKVICV KFEELREVPL
510 520 530 540 550
WVFGLRGLEE LHLEGLFPPE MARGATLESL RELKQLKVLS LRSNAGKVPA
560 570 580 590 600
SVTDVAGHLQ RLSLHNDGAR LLALNSLKKL AVLRELELVA CGLERIPHAI
610 620 630 640 650
FSLGALQELD LKDNHLRSIE EILSFQHCRK LVILRLWHNQ IAYVPEHVRK
660 670 680 690 700
LRSLEQLYLS HNKLETLPAQ LGQCFGLRLL DVSHNGLRSL PPELGLLQSL
710 720 730 740 750
QHLALSYNAL ESLPDELFFC HKLRTLLLGY NHLTQFSPHV AALQALSRLE
760 770 780 790
LKGNRLEALP EELGDCKGLK KSGLLVEDTL YEGLPAEVRE KMEEE
Length:795
Mass (Da):90,445
Last modified:November 8, 2005 - v1
Checksum:i17F6795B243632DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105779 mRNA. Translation: AAI05780.1.
RefSeqiNP_001029311.1. NM_001034139.1.
XP_006248825.1. XM_006248763.3.
XP_006248826.1. XM_006248764.3.
XP_008767176.1. XM_008768954.1.
UniGeneiRn.73867.

Genome annotation databases

EnsembliENSRNOT00000035142; ENSRNOP00000037487; ENSRNOG00000028460.
GeneIDi304203.
KEGGirno:304203.
UCSCiRGD:1311979. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105779 mRNA. Translation: AAI05780.1.
RefSeqiNP_001029311.1. NM_001034139.1.
XP_006248825.1. XM_006248763.3.
XP_006248826.1. XM_006248764.3.
XP_008767176.1. XM_008768954.1.
UniGeneiRn.73867.

3D structure databases

ProteinModelPortaliQ3KRC6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi257792. 1 interactor.
STRINGi10116.ENSRNOP00000037487.

PTM databases

PhosphoSitePlusiQ3KRC6.

Proteomic databases

PaxDbiQ3KRC6.
PRIDEiQ3KRC6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000035142; ENSRNOP00000037487; ENSRNOG00000028460.
GeneIDi304203.
KEGGirno:304203.
UCSCiRGD:1311979. rat.

Organism-specific databases

CTDi80131.
RGDi1311979. Lrrc8e.

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00840000129676.
HOGENOMiHOG000231806.
HOVERGENiHBG052360.
InParanoidiQ3KRC6.
OMAiFINQLCY.
OrthoDBiEOG091G01ER.
PhylomeDBiQ3KRC6.
TreeFamiTF331443.

Miscellaneous databases

PROiQ3KRC6.

Gene expression databases

BgeeiENSRNOG00000028460.
GenevisibleiQ3KRC6. RN.

Family and domain databases

Gene3Di3.80.10.10. 3 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR021040. LRRC8_Pannexin-like.
[Graphical view]
PfamiPF13855. LRR_8. 2 hits.
PF12534. Pannexin_like. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 6 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 8 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLRC8E_RAT
AccessioniPrimary (citable) accession number: Q3KRC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 8, 2005
Last modified: November 30, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.