Q3KRA9 (ALKB6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6
EC=1.14.11.-

Alternative name(s):
Alkylated DNA repair protein alkB homolog 6

Gene names

Name:	ALKBH6
Synonyms:	ABH6

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron By similarity.
Cofactor	Binds 1 Fe2+ ion per subunit By similarity.
Subunit structure	Interacts with METTL21D. Ref.6
Subcellular location	Cytoplasm. Nucleus Ref.1.
Tissue specificity	Widely expressed, with highest expression in testis and pancreas. Ref.1
Sequence similarities	Belongs to the alkB family. Contains 1 Fe2OG dioxygenase domain.
Sequence caution	The sequence AAI05802.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q3KRA9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q3KRA9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAGRGMGMLNLEIGGDAGGRIGCKELVLM

Isoform 3 (identifier: Q3KRA9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAGRGMGMLNLEIGGDAGGRIGCKELVLM
     64-238: LPHPRGMVPE...VLRAGLLLGK → PSGPHCLPQV...LEASQLTMSS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6		PRO_0000323716
Regions
	Domain	96 – 227	132	Fe2OG dioxygenase
	Region	103 – 105	3	Alpha-ketoglutarate binding By similarity
	Region	218 – 224	7	Alpha-ketoglutarate binding By similarity
Sites
	Metal binding	114	1	Iron; catalytic By similarity
	Metal binding	116	1	Iron; catalytic By similarity
	Metal binding	182	1	Iron; catalytic By similarity
Natural variations
	Alternative sequence	1	1	M → MAGRGMGMLNLEIGGDAGGR IGCKELVLM in isoform 2 and isoform 3.		VSP_032056
	Alternative sequence	64 – 238	175	LPHPR…LLLGK → PSGPHCLPQVGFLIPEGWFL SGCPHGSSATWTKCQTSASL EASQLTMSS in isoform 3.		VSP_032057

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 2CA6EEDA8DB61388	FASTA	238	26,483
	10 20 30 40 50 60 MEEQDARVPA LEPFRVEQAP PVIYYVPDFI SKEEEEYLLR QVFNAPKPKW TQLSGRKLQN 70 80 90 100 110 120 WGGLPHPRGM VPERLPPWLQ RYVDKVSNLS LFGGLPANHV LVNQYLPGEG IMPHEDGPLY 130 140 150 160 170 180 YPTVSTISLG SHTVLDFYEP RRPEDDDPTE QPRPPPRPTT SLLLEPRSLL VLRGPAYTRL 190 200 210 220 230 LHGIAAARVD ALDAASSPPN AAACPSARPG ACLVRGTRVS LTIRRVPRVL RAGLLLGK
	Isoform 2 [UniParc]. Checksum: 65D9DAF9D01E59BC	FASTA	266	29,285
	10 20 30 40 50 60 MAGRGMGMLN LEIGGDAGGR IGCKELVLME EQDARVPALE PFRVEQAPPV IYYVPDFISK 70 80 90 100 110 120 EEEEYLLRQV FNAPKPKWTQ LSGRKLQNWG GLPHPRGMVP ERLPPWLQRY VDKVSNLSLF 130 140 150 160 170 180 GGLPANHVLV NQYLPGEGIM PHEDGPLYYP TVSTISLGSH TVLDFYEPRR PEDDDPTEQP 190 200 210 220 230 240 RPPPRPTTSL LLEPRSLLVL RGPAYTRLLH GIAAARVDAL DAASSPPNAA ACPSARPGAC 250 260 LVRGTRVSLT IRRVPRVLRA GLLLGK
	Isoform 3 [UniParc]. Checksum: 6468CF74757A398B	FASTA	140	15,325
	10 20 30 40 50 60 MAGRGMGMLN LEIGGDAGGR IGCKELVLME EQDARVPALE PFRVEQAPPV IYYVPDFISK 70 80 90 100 110 120 EEEEYLLRQV FNAPKPKWTQ LSGRKLQNWG GPSGPHCLPQ VGFLIPEGWF LSGCPHGSSA 130 140 TWTKCQTSAS LEASQLTMSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression and sub-cellular localization of human ABH family molecules." Tsujikawa K., Koike K., Kitae K., Shinkawa A., Arima H., Suzuki T., Tsuchiya M., Makino Y., Furukawa T., Konishi N., Yamamoto H. J. Cell. Mol. Med. 11:1105-1116(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Testis.
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-238 (ISOFORM 2). Tissue: Urinary bladder.
[5]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"Lysine methylation of VCP by a member of a novel human protein methyltransferase family." Kernstock S., Davydova E., Jakobsson M., Moen A., Pettersen S., Maelandsmo G.M., Egge-Jacobsen W., Falnes P.O. Nat. Commun. 3:1038-1038(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH METTL21D.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB273714 mRNA. Translation: BAF63844.1. AF038458 Genomic DNA. No translation available. AK310704 mRNA. No translation available. BC105801 mRNA. Translation: AAI05802.1. Different initiation.
IPI	IPI00394729. IPI00792409. IPI00797675.
RefSeq	NP_116267.3. NM_032878.3.
UniGene	Hs.71941.
3D structure databases
ProteinModelPortal	Q3KRA9.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000368152.
PTM databases
PhosphoSite	Q3KRA9.
Polymorphism databases
DMDM	172046713.
Proteomic databases
PaxDb	Q3KRA9.
PRIDE	Q3KRA9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000252984; ENSP00000252984; ENSG00000239382. ENST00000378875; ENSP00000368152; ENSG00000239382. ENST00000433672; ENSP00000392847; ENSG00000239382. ENST00000475223; ENSP00000464844; ENSG00000239382.
GeneID	84964.
KEGG	hsa:84964.
UCSC	uc002ocv.1. human. uc002ocx.1. human.
Organism-specific databases
CTD	84964.
GeneCards	GC19M036500.
HGNC	HGNC:28243. ALKBH6.
MIM	613304. gene.
neXtProt	NX_Q3KRA9.
PharmGKB	PA143485295.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG254390.
HOGENOM	HOG000210721.
HOVERGEN	HBG056765.
InParanoid	Q3KRA9.
KO	K10768.
OMA	RGTAYTR.
OrthoDB	EOG461450.
Gene expression databases
ArrayExpress	Q3KRA9.
Bgee	Q3KRA9.
CleanEx	HS_ALKBH6.
Genevestigator	Q3KRA9.
Family and domain databases
InterPro	IPR005123. Oxoglu/Fe-dep_dioxygenase. [Graphical view]
Pfam	PF13532. 2OG-FeII_Oxy_2. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	84964.
NextBio	75486.
SOURCE	Search...

Entry information

Entry name

ALKB6_HUMAN

Accession

Primary (citable) accession number: Q3KRA9
Secondary accession number(s): A5LGM8, A6NLP1, A8MU96

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	May 1, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.