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Protein

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6

Gene

ALKBH6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron.By similarity

Cofactori

Fe2+PROSITE-ProRule annotationNote: Binds 1 Fe2+ ion per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi114 – 1141Iron; catalyticPROSITE-ProRule annotation
Metal bindingi116 – 1161Iron; catalyticPROSITE-ProRule annotation
Metal bindingi182 – 1821Iron; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6 (EC:1.14.11.-)
Alternative name(s):
Alkylated DNA repair protein alkB homolog 6
Gene namesi
Name:ALKBH6
Synonyms:ABH6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:28243. ALKBH6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA143485295.

Polymorphism and mutation databases

BioMutaiALKBH6.
DMDMi172046713.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6PRO_0000323716Add
BLAST

Proteomic databases

MaxQBiQ3KRA9.
PaxDbiQ3KRA9.
PRIDEiQ3KRA9.

PTM databases

PhosphoSiteiQ3KRA9.

Expressioni

Tissue specificityi

Widely expressed, with highest expression in testis and pancreas.1 Publication

Gene expression databases

BgeeiQ3KRA9.
CleanExiHS_ALKBH6.
ExpressionAtlasiQ3KRA9. baseline and differential.
GenevisibleiQ3KRA9. HS.

Interactioni

Subunit structurei

Interacts with VCPKMT.1 Publication

Protein-protein interaction databases

STRINGi9606.ENSP00000368152.

Structurei

3D structure databases

ProteinModelPortaliQ3KRA9.
SMRiQ3KRA9. Positions 56-226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini96 – 227132Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni103 – 1053Alpha-ketoglutarate bindingBy similarity
Regioni218 – 2247Alpha-ketoglutarate bindingBy similarity

Sequence similaritiesi

Belongs to the alkB family.Curated
Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG254390.
GeneTreeiENSGT00510000048626.
HOGENOMiHOG000210721.
HOVERGENiHBG056765.
InParanoidiQ3KRA9.
KOiK10768.
OMAiPHPKGML.
PhylomeDBiQ3KRA9.
TreeFamiTF314467.

Family and domain databases

Gene3Di2.60.120.590. 1 hit.
InterProiIPR027450. AlkB-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF13532. 2OG-FeII_Oxy_2. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3KRA9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEQDARVPA LEPFRVEQAP PVIYYVPDFI SKEEEEYLLR QVFNAPKPKW
60 70 80 90 100
TQLSGRKLQN WGGLPHPRGM VPERLPPWLQ RYVDKVSNLS LFGGLPANHV
110 120 130 140 150
LVNQYLPGEG IMPHEDGPLY YPTVSTISLG SHTVLDFYEP RRPEDDDPTE
160 170 180 190 200
QPRPPPRPTT SLLLEPRSLL VLRGPAYTRL LHGIAAARVD ALDAASSPPN
210 220 230
AAACPSARPG ACLVRGTRVS LTIRRVPRVL RAGLLLGK
Length:238
Mass (Da):26,483
Last modified:March 18, 2008 - v2
Checksum:i2CA6EEDA8DB61388
GO
Isoform 2 (identifier: Q3KRA9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAGRGMGMLNLEIGGDAGGRIGCKELVLM

Show »
Length:266
Mass (Da):29,285
Checksum:i65D9DAF9D01E59BC
GO
Isoform 3 (identifier: Q3KRA9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAGRGMGMLNLEIGGDAGGRIGCKELVLM
     64-238: LPHPRGMVPE...VLRAGLLLGK → PSGPHCLPQV...LEASQLTMSS

Show »
Length:140
Mass (Da):15,325
Checksum:i6468CF74757A398B
GO

Sequence cautioni

The sequence AAI05802.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MAGRGMGMLNLEIGGDAGGR IGCKELVLM in isoform 2 and isoform 3. 2 PublicationsVSP_032056
Alternative sequencei64 – 238175LPHPR…LLLGK → PSGPHCLPQVGFLIPEGWFL SGCPHGSSATWTKCQTSASL EASQLTMSS in isoform 3. 1 PublicationVSP_032057Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB273714 mRNA. Translation: BAF63844.1.
AF038458 Genomic DNA. No translation available.
AK310704 mRNA. No translation available.
BC105801 mRNA. Translation: AAI05802.1. Different initiation.
CCDSiCCDS12485.2. [Q3KRA9-2]
CCDS74342.1. [Q3KRA9-1]
RefSeqiNP_001284630.1. NM_001297701.1. [Q3KRA9-1]
NP_116267.3. NM_032878.3. [Q3KRA9-2]
XP_005259414.1. XM_005259357.3. [Q3KRA9-1]
UniGeneiHs.71941.

Genome annotation databases

EnsembliENST00000252984; ENSP00000252984; ENSG00000239382. [Q3KRA9-1]
ENST00000378875; ENSP00000368152; ENSG00000239382. [Q3KRA9-2]
GeneIDi84964.
KEGGihsa:84964.
UCSCiuc002ocv.1. human. [Q3KRA9-2]
uc002ocx.1. human. [Q3KRA9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB273714 mRNA. Translation: BAF63844.1.
AF038458 Genomic DNA. No translation available.
AK310704 mRNA. No translation available.
BC105801 mRNA. Translation: AAI05802.1. Different initiation.
CCDSiCCDS12485.2. [Q3KRA9-2]
CCDS74342.1. [Q3KRA9-1]
RefSeqiNP_001284630.1. NM_001297701.1. [Q3KRA9-1]
NP_116267.3. NM_032878.3. [Q3KRA9-2]
XP_005259414.1. XM_005259357.3. [Q3KRA9-1]
UniGeneiHs.71941.

3D structure databases

ProteinModelPortaliQ3KRA9.
SMRiQ3KRA9. Positions 56-226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000368152.

PTM databases

PhosphoSiteiQ3KRA9.

Polymorphism and mutation databases

BioMutaiALKBH6.
DMDMi172046713.

Proteomic databases

MaxQBiQ3KRA9.
PaxDbiQ3KRA9.
PRIDEiQ3KRA9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000252984; ENSP00000252984; ENSG00000239382. [Q3KRA9-1]
ENST00000378875; ENSP00000368152; ENSG00000239382. [Q3KRA9-2]
GeneIDi84964.
KEGGihsa:84964.
UCSCiuc002ocv.1. human. [Q3KRA9-2]
uc002ocx.1. human. [Q3KRA9-1]

Organism-specific databases

CTDi84964.
GeneCardsiGC19M036500.
HGNCiHGNC:28243. ALKBH6.
MIMi613304. gene.
neXtProtiNX_Q3KRA9.
PharmGKBiPA143485295.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG254390.
GeneTreeiENSGT00510000048626.
HOGENOMiHOG000210721.
HOVERGENiHBG056765.
InParanoidiQ3KRA9.
KOiK10768.
OMAiPHPKGML.
PhylomeDBiQ3KRA9.
TreeFamiTF314467.

Miscellaneous databases

GenomeRNAii84964.
NextBioi75486.
PROiQ3KRA9.
SOURCEiSearch...

Gene expression databases

BgeeiQ3KRA9.
CleanExiHS_ALKBH6.
ExpressionAtlasiQ3KRA9. baseline and differential.
GenevisibleiQ3KRA9. HS.

Family and domain databases

Gene3Di2.60.120.590. 1 hit.
InterProiIPR027450. AlkB-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF13532. 2OG-FeII_Oxy_2. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Testis.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-238 (ISOFORM 2).
    Tissue: Urinary bladder.
  5. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Lysine methylation of VCP by a member of a novel human protein methyltransferase family."
    Kernstock S., Davydova E., Jakobsson M., Moen A., Pettersen S., Maelandsmo G.M., Egge-Jacobsen W., Falnes P.O.
    Nat. Commun. 3:1038-1038(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VCPKMT.

Entry informationi

Entry nameiALKB6_HUMAN
AccessioniPrimary (citable) accession number: Q3KRA9
Secondary accession number(s): A5LGM8, A6NLP1, A8MU96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: June 24, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.