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Q3KRA9 (ALKB6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6

EC=1.14.11.-
Alternative name(s):
Alkylated DNA repair protein alkB homolog 6
Gene names
Name:ALKBH6
Synonyms:ABH6
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length238 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron By similarity.

Cofactor

Binds 1 Fe2+ ion per subunit By similarity.

Subunit structure

Interacts with VCPKMT. Ref.6

Subcellular location

Cytoplasm. Nucleus Ref.1.

Tissue specificity

Widely expressed, with highest expression in testis and pancreas. Ref.1

Sequence similarities

Belongs to the alkB family.

Contains 1 Fe2OG dioxygenase domain.

Sequence caution

The sequence AAI05802.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3KRA9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3KRA9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAGRGMGMLNLEIGGDAGGRIGCKELVLM
Isoform 3 (identifier: Q3KRA9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MAGRGMGMLNLEIGGDAGGRIGCKELVLM
     64-238: LPHPRGMVPE...VLRAGLLLGK → PSGPHCLPQV...LEASQLTMSS

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 238238Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6
PRO_0000323716

Regions

Domain96 – 227132Fe2OG dioxygenase
Region103 – 1053Alpha-ketoglutarate binding By similarity
Region218 – 2247Alpha-ketoglutarate binding By similarity

Sites

Metal binding1141Iron; catalytic By similarity
Metal binding1161Iron; catalytic By similarity
Metal binding1821Iron; catalytic By similarity

Natural variations

Alternative sequence11M → MAGRGMGMLNLEIGGDAGGR IGCKELVLM in isoform 2 and isoform 3.
VSP_032056
Alternative sequence64 – 238175LPHPR…LLLGK → PSGPHCLPQVGFLIPEGWFL SGCPHGSSATWTKCQTSASL EASQLTMSS in isoform 3.
VSP_032057

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 18, 2008. Version 2.
Checksum: 2CA6EEDA8DB61388

FASTA23826,483
        10         20         30         40         50         60 
MEEQDARVPA LEPFRVEQAP PVIYYVPDFI SKEEEEYLLR QVFNAPKPKW TQLSGRKLQN 

        70         80         90        100        110        120 
WGGLPHPRGM VPERLPPWLQ RYVDKVSNLS LFGGLPANHV LVNQYLPGEG IMPHEDGPLY 

       130        140        150        160        170        180 
YPTVSTISLG SHTVLDFYEP RRPEDDDPTE QPRPPPRPTT SLLLEPRSLL VLRGPAYTRL 

       190        200        210        220        230 
LHGIAAARVD ALDAASSPPN AAACPSARPG ACLVRGTRVS LTIRRVPRVL RAGLLLGK 

« Hide

Isoform 2 [UniParc].

Checksum: 65D9DAF9D01E59BC
Show »

FASTA26629,285
Isoform 3 [UniParc].

Checksum: 6468CF74757A398B
Show »

FASTA14015,325

References

« Hide 'large scale' references
[1]"Expression and sub-cellular localization of human ABH family molecules."
Tsujikawa K., Koike K., Kitae K., Shinkawa A., Arima H., Suzuki T., Tsuchiya M., Makino Y., Furukawa T., Konishi N., Yamamoto H.
J. Cell. Mol. Med. 11:1105-1116(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Testis.
[2]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Testis.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-238 (ISOFORM 2).
Tissue: Urinary bladder.
[5]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Lysine methylation of VCP by a member of a novel human protein methyltransferase family."
Kernstock S., Davydova E., Jakobsson M., Moen A., Pettersen S., Maelandsmo G.M., Egge-Jacobsen W., Falnes P.O.
Nat. Commun. 3:1038-1038(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH VCPKMT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB273714 mRNA. Translation: BAF63844.1.
AF038458 Genomic DNA. No translation available.
AK310704 mRNA. No translation available.
BC105801 mRNA. Translation: AAI05802.1. Different initiation.
RefSeqNP_116267.3. NM_032878.3.
XP_005259414.1. XM_005259357.2.
XP_005259415.1. XM_005259358.2.
UniGeneHs.71941.

3D structure databases

ProteinModelPortalQ3KRA9.
SMRQ3KRA9. Positions 13-229.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000368152.

PTM databases

PhosphoSiteQ3KRA9.

Polymorphism databases

DMDM172046713.

Proteomic databases

PaxDbQ3KRA9.
PRIDEQ3KRA9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000252984; ENSP00000252984; ENSG00000239382. [Q3KRA9-1]
ENST00000378875; ENSP00000368152; ENSG00000239382. [Q3KRA9-2]
GeneID84964.
KEGGhsa:84964.
UCSCuc002ocv.1. human. [Q3KRA9-2]
uc002ocx.1. human. [Q3KRA9-1]

Organism-specific databases

CTD84964.
GeneCardsGC19M036500.
HGNCHGNC:28243. ALKBH6.
MIM613304. gene.
neXtProtNX_Q3KRA9.
PharmGKBPA143485295.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG254390.
HOGENOMHOG000210721.
HOVERGENHBG056765.
InParanoidQ3KRA9.
KOK10768.
OMAGKTANHV.
PhylomeDBQ3KRA9.
TreeFamTF314467.

Gene expression databases

ArrayExpressQ3KRA9.
BgeeQ3KRA9.
CleanExHS_ALKBH6.
GenevestigatorQ3KRA9.

Family and domain databases

Gene3D2.60.120.590. 1 hit.
InterProIPR027450. AlkB-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamPF13532. 2OG-FeII_Oxy_2. 1 hit.
[Graphical view]
PROSITEPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi84964.
NextBio75486.
PROQ3KRA9.
SOURCESearch...

Entry information

Entry nameALKB6_HUMAN
AccessionPrimary (citable) accession number: Q3KRA9
Secondary accession number(s): A5LGM8, A6NLP1, A8MU96
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: April 16, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM