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Protein

Protein Spindly

Gene

Spdl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SpindlyUniRule annotation
Alternative name(s):
Coiled-coil domain-containing protein 99UniRule annotation
Spindle apparatus coiled-coil domain-containing protein 1UniRule annotation
Gene namesi
Name:Spdl1
Synonyms:Ccdc99
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1306908. Spdl1.

Subcellular locationi

  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome UniRule annotation
  • Chromosomecentromerekinetochore UniRule annotation
  • Nucleus UniRule annotation
  • Cytoplasmcytoskeletonspindle pole UniRule annotation

  • Note: Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 597597Protein SpindlyPRO_0000274519Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei508 – 5081PhosphoserineBy similarity
Modified residuei547 – 5471PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ3KR99.
PRIDEiQ3KR99.

PTM databases

iPTMnetiQ3KR99.
PhosphoSiteiQ3KR99.

Expressioni

Gene expression databases

BgeeiENSRNOG00000007292.
GenevisibleiQ3KR99. RN.

Interactioni

Subunit structurei

Interacts with KNTC1 and ZW10. These interactions appear weak and may be transient or indirect.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009648.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1 – 445445UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the Spindly family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IDXF. Eukaryota.
ENOG4110EUG. LUCA.
GeneTreeiENSGT00510000047951.
HOGENOMiHOG000070239.
InParanoidiQ3KR99.
OMAiCECEAIK.
OrthoDBiEOG091G08GL.
PhylomeDBiQ3KR99.
TreeFamiTF332470.

Family and domain databases

HAMAPiMF_03041. SPDLY. 1 hit.
InterProiIPR028593. SPDLY_chordates.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3KR99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEADITNLRN KLKECEDERL KAAQYGLQLL ERQTELQSQL DKCHEEMMTT
60 70 80 90 100
AENYNQEKYA LQREVELKSR MLESLSCECE ALRQQQKAQL EQLEMQLHRS
110 120 130 140 150
HRQEVHGLRN KLENLKVELD EARLSEKQLK QKLDHQGELL SHKSEELRLL
160 170 180 190 200
SEQRALSSVS SELLSLQTEL TEAEGVKNAL REEVNELQCK QEQLECLNAS
210 220 230 240 250
LLHQVDRLKE EKEEREKEAV SYYNALEKAR VENQDLQVQL GHALQQAADP
260 270 280 290 300
NSKGNSLFAE VEDRRVAMER RLNLMKDKYQ SLKKQNAFTR DQMNKMKLQI
310 320 330 340 350
STLLRMRGSQ TEFEQQERLF AMLEQKNGEI KHLLGEINKL EKFKNLYENM
360 370 380 390 400
ESKSSTSDSA CALEDSTYYT DLLQLKLDKL NKENESTKGE LSIQRMKALF
410 420 430 440 450
ESQRTLDIER KLFANERHLQ LSESENLKLR AKLDELKLKY EPEERIEVPV
460 470 480 490 500
LKRRREVLPL NINTPEETAA ASATGEDVSR LPLERKEESC LDNLKDNTVQ
510 520 530 540 550
WKQPASLSPH KSLPLDTQPK KEKCVTLVAS PASTEVLHEQ SGNTPSSPRL
560 570 580 590
TEESRLPTKV KERKEATSKL EKGASKKSHT IMYVSSKSTP EMQCPQQ
Length:597
Mass (Da):69,112
Last modified:November 8, 2005 - v1
Checksum:i8405161BE73F7235
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105812 mRNA. Translation: AAI05813.1.
RefSeqiNP_001029310.1. NM_001034138.1.
XP_006246166.1. XM_006246104.1.
UniGeneiRn.54949.

Genome annotation databases

EnsembliENSRNOT00000009648; ENSRNOP00000009648; ENSRNOG00000007292.
GeneIDi303037.
KEGGirno:303037.
UCSCiRGD:1306908. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105812 mRNA. Translation: AAI05813.1.
RefSeqiNP_001029310.1. NM_001034138.1.
XP_006246166.1. XM_006246104.1.
UniGeneiRn.54949.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009648.

PTM databases

iPTMnetiQ3KR99.
PhosphoSiteiQ3KR99.

Proteomic databases

PaxDbiQ3KR99.
PRIDEiQ3KR99.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009648; ENSRNOP00000009648; ENSRNOG00000007292.
GeneIDi303037.
KEGGirno:303037.
UCSCiRGD:1306908. rat.

Organism-specific databases

CTDi54908.
RGDi1306908. Spdl1.

Phylogenomic databases

eggNOGiENOG410IDXF. Eukaryota.
ENOG4110EUG. LUCA.
GeneTreeiENSGT00510000047951.
HOGENOMiHOG000070239.
InParanoidiQ3KR99.
OMAiCECEAIK.
OrthoDBiEOG091G08GL.
PhylomeDBiQ3KR99.
TreeFamiTF332470.

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.

Miscellaneous databases

PROiQ3KR99.

Gene expression databases

BgeeiENSRNOG00000007292.
GenevisibleiQ3KR99. RN.

Family and domain databases

HAMAPiMF_03041. SPDLY. 1 hit.
InterProiIPR028593. SPDLY_chordates.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPDLY_RAT
AccessioniPrimary (citable) accession number: Q3KR99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.