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Protein

Protein Spindly

Gene

Spdl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (By similarity).UniRule annotationBy similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-RNO-141444. Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
R-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SpindlyUniRule annotation
Alternative name(s):
Coiled-coil domain-containing protein 99UniRule annotation
Spindle apparatus coiled-coil domain-containing protein 1UniRule annotation
Gene namesi
Name:Spdl1
Synonyms:Ccdc99
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1306908. Spdl1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002745191 – 597Protein SpindlyAdd BLAST597

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei508PhosphoserineBy similarity1
Modified residuei547PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ3KR99.
PRIDEiQ3KR99.

PTM databases

iPTMnetiQ3KR99.
PhosphoSitePlusiQ3KR99.

Expressioni

Gene expression databases

BgeeiENSRNOG00000007292.
GenevisibleiQ3KR99. RN.

Interactioni

Subunit structurei

Interacts with KNTC1 and ZW10. These interactions appear weak and may be transient or indirect. Interacts with dynein intermediate chain and dynactin (DCTN1) (By similarity).UniRule annotationBy similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009648.

Structurei

3D structure databases

SMRiQ3KR99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1 – 445UniRule annotationAdd BLAST445

Sequence similaritiesi

Belongs to the Spindly family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IDXF. Eukaryota.
ENOG4110EUG. LUCA.
GeneTreeiENSGT00510000047951.
HOGENOMiHOG000070239.
InParanoidiQ3KR99.
OMAiCECEAIK.
OrthoDBiEOG091G08GL.
PhylomeDBiQ3KR99.
TreeFamiTF332470.

Family and domain databases

HAMAPiMF_03041. SPDLY. 1 hit.
InterProiView protein in InterPro
IPR028593. SPDLY_chordates.

Sequencei

Sequence statusi: Complete.

Q3KR99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEADITNLRN KLKECEDERL KAAQYGLQLL ERQTELQSQL DKCHEEMMTT
60 70 80 90 100
AENYNQEKYA LQREVELKSR MLESLSCECE ALRQQQKAQL EQLEMQLHRS
110 120 130 140 150
HRQEVHGLRN KLENLKVELD EARLSEKQLK QKLDHQGELL SHKSEELRLL
160 170 180 190 200
SEQRALSSVS SELLSLQTEL TEAEGVKNAL REEVNELQCK QEQLECLNAS
210 220 230 240 250
LLHQVDRLKE EKEEREKEAV SYYNALEKAR VENQDLQVQL GHALQQAADP
260 270 280 290 300
NSKGNSLFAE VEDRRVAMER RLNLMKDKYQ SLKKQNAFTR DQMNKMKLQI
310 320 330 340 350
STLLRMRGSQ TEFEQQERLF AMLEQKNGEI KHLLGEINKL EKFKNLYENM
360 370 380 390 400
ESKSSTSDSA CALEDSTYYT DLLQLKLDKL NKENESTKGE LSIQRMKALF
410 420 430 440 450
ESQRTLDIER KLFANERHLQ LSESENLKLR AKLDELKLKY EPEERIEVPV
460 470 480 490 500
LKRRREVLPL NINTPEETAA ASATGEDVSR LPLERKEESC LDNLKDNTVQ
510 520 530 540 550
WKQPASLSPH KSLPLDTQPK KEKCVTLVAS PASTEVLHEQ SGNTPSSPRL
560 570 580 590
TEESRLPTKV KERKEATSKL EKGASKKSHT IMYVSSKSTP EMQCPQQ
Length:597
Mass (Da):69,112
Last modified:November 8, 2005 - v1
Checksum:i8405161BE73F7235
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105812 mRNA. Translation: AAI05813.1.
RefSeqiNP_001029310.1. NM_001034138.1.
XP_006246166.1. XM_006246104.1.
UniGeneiRn.54949.

Genome annotation databases

EnsembliENSRNOT00000009648; ENSRNOP00000009648; ENSRNOG00000007292.
GeneIDi303037.
KEGGirno:303037.
UCSCiRGD:1306908. rat.

Similar proteinsi

Entry informationi

Entry nameiSPDLY_RAT
AccessioniPrimary (citable) accession number: Q3KR99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: November 8, 2005
Last modified: November 22, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families