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Protein

Protein-tyrosine sulfotransferase 1

Gene

Tpst1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.By similarity

Catalytic activityi

3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei79 – 791By similarity
Active sitei100 – 1001By similarity
Sitei159 – 1591Transition state stabilizerBy similarity
Sitei286 – 2861Transition state stabilizerBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-tyrosine sulfotransferase 1 (EC:2.8.2.20)
Alternative name(s):
Tyrosylprotein sulfotransferase 1
Short name:
TPST-1
Gene namesi
Name:Tpst1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1308473. Tpst1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence analysis
Transmembranei9 – 2517Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini26 – 370345LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Protein-tyrosine sulfotransferase 1PRO_0000253722Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi60 – 601N-linked (GlcNAc...)Sequence analysis
Disulfide bondi97 ↔ 157By similarity
Disulfide bondi226 ↔ 234By similarity
Glycosylationi262 – 2621N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ3KR92.
PRIDEiQ3KR92.

Expressioni

Gene expression databases

ExpressionAtlasiQ3KR92. baseline and differential.
GenevisibleiQ3KR92. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4579848.
STRINGi10116.ENSRNOP00000060104.

Family & Domainsi

Sequence similaritiesi

Belongs to the protein sulfotransferase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3988. Eukaryota.
ENOG410XQ2X. LUCA.
GeneTreeiENSGT00390000006030.
HOGENOMiHOG000253020.
HOVERGENiHBG001270.
InParanoidiQ3KR92.
KOiK01021.
OMAiYNKDMPL.
PhylomeDBiQ3KR92.
TreeFamiTF312910.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR026634. TPST.
[Graphical view]
PANTHERiPTHR12788. PTHR12788. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q3KR92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGKLKQNLL LACLVISSVT VFYLGQHAME CHHRIEERSQ PARLENPKAT
60 70 80 90 100
VRTGLDIKAN KTFTYHKNMP LIFIGGVPRS GTTLMRAMLD AHPDIRCGEE
110 120 130 140 150
TRVIPRILAL KQMWSRSSKE KIRLDEAGVT DEVLDSAMQA FLLEVIVKHG
160 170 180 190 200
EPAPYLCNKD PFALKSLTYL ARLFPNAKFL LMVRDGRASV HSMISRKVTI
210 220 230 240 250
AGFDLNSYRD CLTKWNRAIE TMYNQCMEVG YKKCMLVHYE QLVLHPERWM
260 270 280 290 300
RTLLKFLHIP WNHSVLHHEE MIGKAGGVSL SKVERSTDQV IKPVNVGALS
310 320 330 340 350
KWVGKIPPDV LQDMAVIAPM LAKLGYDPYA NPPNYGKPDP KILENTRRVH
360 370
KGEFQLPDFL KEKPQTEQVE
Length:370
Mass (Da):42,135
Last modified:November 8, 2005 - v1
Checksum:iA14A57DD5E62ED2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105826 mRNA. Translation: AAI05827.1.
RefSeqiNP_001011903.2. NM_001011903.2.
XP_008767355.1. XM_008769133.1.
UniGeneiRn.144004.

Genome annotation databases

EnsembliENSRNOT00000065868; ENSRNOP00000060104; ENSRNOG00000000900.
ENSRNOT00000084968; ENSRNOP00000070504; ENSRNOG00000000900.
GeneIDi288617.
KEGGirno:288617.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105826 mRNA. Translation: AAI05827.1.
RefSeqiNP_001011903.2. NM_001011903.2.
XP_008767355.1. XM_008769133.1.
UniGeneiRn.144004.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4579848.
STRINGi10116.ENSRNOP00000060104.

Proteomic databases

PaxDbiQ3KR92.
PRIDEiQ3KR92.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000065868; ENSRNOP00000060104; ENSRNOG00000000900.
ENSRNOT00000084968; ENSRNOP00000070504; ENSRNOG00000000900.
GeneIDi288617.
KEGGirno:288617.

Organism-specific databases

CTDi8460.
RGDi1308473. Tpst1.

Phylogenomic databases

eggNOGiKOG3988. Eukaryota.
ENOG410XQ2X. LUCA.
GeneTreeiENSGT00390000006030.
HOGENOMiHOG000253020.
HOVERGENiHBG001270.
InParanoidiQ3KR92.
KOiK01021.
OMAiYNKDMPL.
PhylomeDBiQ3KR92.
TreeFamiTF312910.

Miscellaneous databases

NextBioi628431.
PROiQ3KR92.

Gene expression databases

ExpressionAtlasiQ3KR92. baseline and differential.
GenevisibleiQ3KR92. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR026634. TPST.
[Graphical view]
PANTHERiPTHR12788. PTHR12788. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.

Entry informationi

Entry nameiTPST1_RAT
AccessioniPrimary (citable) accession number: Q3KR92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: November 8, 2005
Last modified: May 11, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.