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Protein

Protein-tyrosine sulfotransferase 1

Gene

Tpst1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei100Proton donor/acceptorBy similarity1
Sitei159Transition state stabilizerBy similarity1
Binding sitei184PAPSBy similarity1
Binding sitei192PAPSBy similarity1
Binding sitei196PAPSBy similarity1
Binding sitei239PAPSBy similarity1
Sitei286Transition state stabilizerBy similarity1
Binding sitei301PAPSBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

ReactomeiR-RNO-156584 Cytosolic sulfonation of small molecules

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-tyrosine sulfotransferase 1 (EC:2.8.2.201 Publication)
Alternative name(s):
Tyrosylprotein sulfotransferase 1
Short name:
TPST-1
Gene namesi
Name:Tpst1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1308473 Tpst1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini26 – 370LumenalSequence analysisAdd BLAST345

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002537221 – 370Protein-tyrosine sulfotransferase 1Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi60N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi97 ↔ 157By similarity
Disulfide bondi226 ↔ 234By similarity
Glycosylationi262N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ3KR92
PRIDEiQ3KR92

Expressioni

Gene expression databases

BgeeiENSRNOG00000000900
ExpressionAtlasiQ3KR92 baseline and differential
GenevisibleiQ3KR92 RN

Interactioni

Subunit structurei

Homodimer. Can also form heterodimers with TPST2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000060104

Structurei

3D structure databases

SMRiQ3KR92
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 83PAPS bindingBy similarity5
Regioni102 – 106Interaction with peptide substrateBy similarity5
Regioni286 – 295PAPS bindingBy similarity10

Sequence similaritiesi

Belongs to the protein sulfotransferase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3988 Eukaryota
ENOG410XQ2X LUCA
GeneTreeiENSGT00390000006030
HOGENOMiHOG000253020
HOVERGENiHBG001270
InParanoidiQ3KR92
KOiK01021
OMAiHIPWNHS
OrthoDBiEOG091G09YJ
PhylomeDBiQ3KR92
TreeFamiTF312910

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR026634 TPST
PANTHERiPTHR12788 PTHR12788, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits

Sequencei

Sequence statusi: Complete.

Q3KR92-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGKLKQNLL LACLVISSVT VFYLGQHAME CHHRIEERSQ PARLENPKAT
60 70 80 90 100
VRTGLDIKAN KTFTYHKNMP LIFIGGVPRS GTTLMRAMLD AHPDIRCGEE
110 120 130 140 150
TRVIPRILAL KQMWSRSSKE KIRLDEAGVT DEVLDSAMQA FLLEVIVKHG
160 170 180 190 200
EPAPYLCNKD PFALKSLTYL ARLFPNAKFL LMVRDGRASV HSMISRKVTI
210 220 230 240 250
AGFDLNSYRD CLTKWNRAIE TMYNQCMEVG YKKCMLVHYE QLVLHPERWM
260 270 280 290 300
RTLLKFLHIP WNHSVLHHEE MIGKAGGVSL SKVERSTDQV IKPVNVGALS
310 320 330 340 350
KWVGKIPPDV LQDMAVIAPM LAKLGYDPYA NPPNYGKPDP KILENTRRVH
360 370
KGEFQLPDFL KEKPQTEQVE
Length:370
Mass (Da):42,135
Last modified:November 8, 2005 - v1
Checksum:iA14A57DD5E62ED2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105826 mRNA Translation: AAI05827.1
RefSeqiNP_001011903.2, NM_001011903.2
XP_008767355.1, XM_008769133.2
UniGeneiRn.144004

Genome annotation databases

EnsembliENSRNOT00000065868; ENSRNOP00000060104; ENSRNOG00000000900
ENSRNOT00000084968; ENSRNOP00000070504; ENSRNOG00000000900
GeneIDi288617
KEGGirno:288617

Similar proteinsi

Entry informationi

Entry nameiTPST1_RAT
AccessioniPrimary (citable) accession number: Q3KR92
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: November 8, 2005
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health