Q3KR37 (GRM1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GRAM domain-containing protein 1B

Gene names

Name:	GRAMD1B
Synonyms:	KIAA1201
ORF Names:	UNQ3032/PRO9834

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Contains 1 GRAM domain.
Sequence caution	The sequence BAA86515.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q3KR37-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q3KR37-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q3KR37-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-309: Missing.
     310-361: EGDGSLEKEL...LSDSSDTHDE → MPTSSAVLLR...CLLPDPLFCA
     631-634: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 738	738	GRAM domain-containing protein 1B		PRO_0000287449
Regions
	Transmembrane	623 – 643	21	Helical; Potential
	Domain	96 – 163	68	GRAM
Amino acid modifications
	Modified residue	21	1	Phosphoserine By similarity
	Modified residue	274	1	Phosphoserine Ref.4
	Modified residue	389	1	Phosphotyrosine Ref.4
	Modified residue	581	1	Phosphoserine Ref.4
	Modified residue	585	1	Phosphothreonine Ref.4
Natural variations
	Alternative sequence	1 – 309	309	Missing in isoform 3.		VSP_025469
	Alternative sequence	1 – 40	40	Missing in isoform 2.		VSP_025470
	Alternative sequence	310 – 361	52	EGDGS…DTHDE → MPTSSAVLLRVLSIPLLTVL ILARDLSALGGCPWGPLPLR CHCLLPDPLFCA in isoform 3.		VSP_025471
	Alternative sequence	631 – 634	4	Missing in isoform 3.		VSP_025472

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 96784D4504307393	FASTA	738	85,400
	10 20 30 40 50 60 MKGFKLSCTA SNSNRSTPAC SPILRKRSRS PTPQNQDGDT MVEKGSDHSS DKSPSTPEQG 70 80 90 100 110 120 VQRSCSSQSG RSGGKNSKKS QSWYNVLSPT YKQRNEDFRK LFKQLPDTER LIVDYSCALQ 130 140 150 160 170 180 RDILLQGRLY LSENWICFYS NIFRWETLLT VRLKDICSMT KEKTARLIPN AIQVCTDSEK 190 200 210 220 230 240 HFFTSFGARD RTYMMMFRLW QNALLEKPLC PKELWHFVHQ CYGNELGLTS DDEDYVPPDD 250 260 270 280 290 300 DFNTMGYCEE IPVEENEVND SSSKSSIETK PDASPQLPKK SITNSTLTST GSSEAPVSFD 310 320 330 340 350 360 GLPLEEEALE GDGSLEKELA IDNIMGEKIE MIAPVNSPSL DFNDNEDIPT ELSDSSDTHD 370 380 390 400 410 420 EGEVQAFYED LSGRQYVNEV FNFSVDKLYD LLFTNSPFQR DFMEQRRFSD IIFHPWKKEE 430 440 450 460 470 480 NGNQSRVILY TITLTNPLAP KTATVRETQT MYKASQESEC YVIDAEVLTH DVPYHDYFYT 490 500 510 520 530 540 INRYTLTRVA RNKSRLRVST ELRYRKQPWG LVKTFIEKNF WSGLEDYFRH LESELAKTES 550 560 570 580 590 600 TYLAEMHRQS PKEKASKTTT VRRRKRPHAH LRVPHLEEVM SPVTTPTDED VGHRIKHVAG 610 620 630 640 650 660 STQTRHIPED TPNGFHLQSV SKLLLVISCV ICFSLVLLVI LNMMLFYKLW MLEYTTQTLT 670 680 690 700 710 720 AWQGLRLQER LPQSQTEWAQ LLESQQKYHD TELQKWREII KSSVMLLDQM KDSLINLQNG 730 IRSRDYTSES EEKRNRYH
	Isoform 2 [UniParc]. Checksum: 29D47C008D52509A	FASTA	698	81,079
	10 20 30 40 50 60 MVEKGSDHSS DKSPSTPEQG VQRSCSSQSG RSGGKNSKKS QSWYNVLSPT YKQRNEDFRK 70 80 90 100 110 120 LFKQLPDTER LIVDYSCALQ RDILLQGRLY LSENWICFYS NIFRWETLLT VRLKDICSMT 130 140 150 160 170 180 KEKTARLIPN AIQVCTDSEK HFFTSFGARD RTYMMMFRLW QNALLEKPLC PKELWHFVHQ 190 200 210 220 230 240 CYGNELGLTS DDEDYVPPDD DFNTMGYCEE IPVEENEVND SSSKSSIETK PDASPQLPKK 250 260 270 280 290 300 SITNSTLTST GSSEAPVSFD GLPLEEEALE GDGSLEKELA IDNIMGEKIE MIAPVNSPSL 310 320 330 340 350 360 DFNDNEDIPT ELSDSSDTHD EGEVQAFYED LSGRQYVNEV FNFSVDKLYD LLFTNSPFQR 370 380 390 400 410 420 DFMEQRRFSD IIFHPWKKEE NGNQSRVILY TITLTNPLAP KTATVRETQT MYKASQESEC 430 440 450 460 470 480 YVIDAEVLTH DVPYHDYFYT INRYTLTRVA RNKSRLRVST ELRYRKQPWG LVKTFIEKNF 490 500 510 520 530 540 WSGLEDYFRH LESELAKTES TYLAEMHRQS PKEKASKTTT VRRRKRPHAH LRVPHLEEVM 550 560 570 580 590 600 SPVTTPTDED VGHRIKHVAG STQTRHIPED TPNGFHLQSV SKLLLVISCV ICFSLVLLVI 610 620 630 640 650 660 LNMMLFYKLW MLEYTTQTLT AWQGLRLQER LPQSQTEWAQ LLESQQKYHD TELQKWREII 670 680 690 KSSVMLLDQM KDSLINLQNG IRSRDYTSES EEKRNRYH
	Isoform 3 [UniParc]. Checksum: BDE9CC95661A6D4B	FASTA	425	49,786
	10 20 30 40 50 60 MPTSSAVLLR VLSIPLLTVL ILARDLSALG GCPWGPLPLR CHCLLPDPLF CAGEVQAFYE 70 80 90 100 110 120 DLSGRQYVNE VFNFSVDKLY DLLFTNSPFQ RDFMEQRRFS DIIFHPWKKE ENGNQSRVIL 130 140 150 160 170 180 YTITLTNPLA PKTATVRETQ TMYKASQESE CYVIDAEVLT HDVPYHDYFY TINRYTLTRV 190 200 210 220 230 240 ARNKSRLRVS TELRYRKQPW GLVKTFIEKN FWSGLEDYFR HLESELAKTE STYLAEMHRQ 250 260 270 280 290 300 SPKEKASKTT TVRRRKRPHA HLRVPHLEEV MSPVTTPTDE DVGHRIKHVA GSTQTRHIPE 310 320 330 340 350 360 DTPNGFHLQS VSKLLLVISC VLVLLVILNM MLFYKLWMLE YTTQTLTAWQ GLRLQERLPQ 370 380 390 400 410 420 SQTEWAQLLE SQQKYHDTEL QKWREIIKSS VMLLDQMKDS LINLQNGIRS RDYTSESEEK RNRYH

References

[1]	"Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O. DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-274; TYR-389; SER-581 AND THR-585, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB033027 mRNA. Translation: BAA86515.1. Different initiation. AY358924 mRNA. Translation: AAQ89283.1. BC105741 mRNA. Translation: AAI05742.1. BC105932 mRNA. Translation: AAI05933.1. BC105931 mRNA. Translation: AAI05932.1. BC107480 mRNA. Translation: AAI07481.1.
IPI	IPI00432073. IPI00845280. IPI00939399.
RefSeq	NP_065767.1. NM_020716.1.
UniGene	Hs.144725. Hs.732509.
3D structure databases
ProteinModelPortal	Q3KR37.
ModBase	Search...
PTM databases
PhosphoSite	Q3KR37.
Polymorphism databases
DMDM	121942617.
Proteomic databases
PaxDb	Q3KR37.
PRIDE	Q3KR37.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000450171; ENSP00000388458; ENSG00000023171. ENST00000529432; ENSP00000432987; ENSG00000023171. ENST00000529750; ENSP00000436500; ENSG00000023171.
GeneID	57476.
KEGG	hsa:57476.
UCSC	uc001pyx.2. human. uc001pyy.2. human.
Organism-specific databases
CTD	57476.
GeneCards	GC11P123396.
HGNC	HGNC:29214. GRAMD1B.
HPA	HPA008557.
neXtProt	NX_Q3KR37.
PharmGKB	PA142671708.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG304071.
HOGENOM	HOG000294164.
HOVERGEN	HBG062004.
InParanoid	Q3KR37.
Gene expression databases
ArrayExpress	Q3KR37.
Bgee	Q3KR37.
CleanEx	HS_GRAMD1B.
Genevestigator	Q3KR37.
Family and domain databases
InterPro	IPR004182. GRAM. [Graphical view]
Pfam	PF02893. GRAM. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	57476.
NextBio	63719.

Entry information

Entry name

GRM1B_HUMAN

Accession

Primary (citable) accession number: Q3KR37
Secondary accession number(s): Q6UW85, Q9ULL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	November 8, 2005
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.