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Protein

E3 ubiquitin-protein ligase MARCH5

Gene

March5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri6 – 75RING-CH-typePROSITE-ProRule annotationAdd BLAST70

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MARCH5 (EC:2.3.2.27)
Alternative name(s):
Membrane-associated RING finger protein 5
Membrane-associated RING-CH protein V
Short name:
MARCH-V
RING-type E3 ubiquitin transferase MARCH5Curated
Gene namesi
Name:March5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1915207 March5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei99 – 119HelicalSequence analysisAdd BLAST21
Transmembranei139 – 159HelicalSequence analysisAdd BLAST21
Transmembranei209 – 229HelicalSequence analysisAdd BLAST21
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002717701 – 278E3 ubiquitin-protein ligase MARCH5Add BLAST278

Post-translational modificationi

Autoubiquitinated leading to degradation.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ3KNM2
MaxQBiQ3KNM2
PaxDbiQ3KNM2
PRIDEiQ3KNM2

PTM databases

iPTMnetiQ3KNM2
PhosphoSitePlusiQ3KNM2
SwissPalmiQ3KNM2

Expressioni

Gene expression databases

BgeeiENSMUSG00000023307
CleanExiMM_MARCH5
ExpressionAtlasiQ3KNM2 baseline and differential
GenevisibleiQ3KNM2 MM

Interactioni

Subunit structurei

Monomer and homodimer. Interacts with MFN1, MFN2, DNM1L and FIS1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213232, 4 interactors
STRINGi10090.ENSMUSP00000024078

Structurei

3D structure databases

ProteinModelPortaliQ3KNM2
SMRiQ3KNM2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-CH-type zinc finger domain is required for E3 ligase activity.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri6 – 75RING-CH-typePROSITE-ProRule annotationAdd BLAST70

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG3053 Eukaryota
ENOG410XSJW LUCA
GeneTreeiENSGT00390000009948
HOGENOMiHOG000247040
HOVERGENiHBG059795
InParanoidiQ3KNM2
KOiK10660
OMAiKQYGRRK
OrthoDBiEOG091G0G4K
PhylomeDBiQ3KNM2
TreeFamiTF316219

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR033275 MARCH-like
IPR011016 Znf_RING-CH
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR23012 PTHR23012, 1 hit
PfamiView protein in Pfam
PF12906 RINGv, 1 hit
SMARTiView protein in SMART
SM00744 RINGv, 1 hit
PROSITEiView protein in PROSITE
PS51292 ZF_RING_CH, 1 hit

Sequencei

Sequence statusi: Complete.

Q3KNM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDQALQQML DRSCWVCFAT DEDDRTAEWV RPCRCRGSTK WVHQACLQRW
60 70 80 90 100
VDEKQRGNST ARVACPQCNA EYLIVFPKLG PVVYVLDLAD RLISKACPFA
110 120 130 140 150
AAGIMVGSIY WTAVTYGAVT VMQVVGHKEG LDVMERADPL FLLIGLPTIP
160 170 180 190 200
VMLILGKMIR WEDYVLRLWR KYSNKLQILN SIFPGIGCPV PRIPAEANPL
210 220 230 240 250
ADHVSATRIL CGALVFPTIA TIVGKLMFSS VNSNLQRTIL GGIAFVAIKG
260 270
AFKVYFKQQQ YLRQAHRKIL NYPEQEEA
Length:278
Mass (Da):31,232
Last modified:November 8, 2005 - v1
Checksum:iCB00A408E228A9EE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85V → A in AAH64752 (PubMed:15489334).Curated1
Sequence conflicti241 – 244GGIA → VRWI in BAB26154 (PubMed:16141072).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003478 mRNA Translation: BAB22809.1
AK009232 mRNA Translation: BAB26154.1
BC064752 mRNA Translation: AAH64752.1
BC107215 mRNA Translation: AAI07216.1
CCDSiCCDS29776.1
RefSeqiNP_001157808.1, NM_001164336.1
NP_081590.3, NM_027314.3
UniGeneiMm.175989

Genome annotation databases

EnsembliENSMUST00000024078; ENSMUSP00000024078; ENSMUSG00000023307
ENSMUST00000215066; ENSMUSP00000150553; ENSMUSG00000111123
GeneIDi69104
KEGGimmu:69104
UCSCiuc008hif.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMARH5_MOUSE
AccessioniPrimary (citable) accession number: Q3KNM2
Secondary accession number(s): Q3KNM3
, Q6P230, Q9CPS3, Q9CTI6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 8, 2005
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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