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Protein

E3 ubiquitin-protein ligase MARCH5

Gene

March5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri6 – 75RING-CH-typePROSITE-ProRule annotationAdd BLAST70

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MARCH5 (EC:2.3.2.27)
Alternative name(s):
Membrane-associated RING finger protein 5
Membrane-associated RING-CH protein V
Short name:
MARCH-V
RING-type E3 ubiquitin transferase MARCH5Curated
Gene namesi
Name:March5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1915207. March5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei99 – 119HelicalSequence analysisAdd BLAST21
Transmembranei139 – 159HelicalSequence analysisAdd BLAST21
Transmembranei209 – 229HelicalSequence analysisAdd BLAST21
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002717701 – 278E3 ubiquitin-protein ligase MARCH5Add BLAST278

Post-translational modificationi

Autoubiquitinated leading to degradation.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiQ3KNM2.
MaxQBiQ3KNM2.
PaxDbiQ3KNM2.
PRIDEiQ3KNM2.

PTM databases

iPTMnetiQ3KNM2.
PhosphoSitePlusiQ3KNM2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000023307.
CleanExiMM_MARCH5.
ExpressionAtlasiQ3KNM2. baseline and differential.
GenevisibleiQ3KNM2. MM.

Interactioni

Subunit structurei

Monomer and homodimer. Interacts with MFN1, MFN2, DNM1L and FIS1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213232. 4 interactors.
STRINGi10090.ENSMUSP00000024078.

Structurei

3D structure databases

ProteinModelPortaliQ3KNM2.
SMRiQ3KNM2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-CH-type zinc finger domain is required for E3 ligase activity.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri6 – 75RING-CH-typePROSITE-ProRule annotationAdd BLAST70

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG3053. Eukaryota.
ENOG410XSJW. LUCA.
GeneTreeiENSGT00390000009948.
HOGENOMiHOG000247040.
HOVERGENiHBG059795.
InParanoidiQ3KNM2.
KOiK10660.
OMAiKQYGRRK.
OrthoDBiEOG091G0G4K.
PhylomeDBiQ3KNM2.
TreeFamiTF316219.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR033275. MARCH-like.
IPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
PANTHERiPTHR23012. PTHR23012. 1 hit.
PfamiView protein in Pfam
PF12906. RINGv. 1 hit.
SMARTiView protein in SMART
SM00744. RINGv. 1 hit.
PROSITEiView protein in PROSITE
PS51292. ZF_RING_CH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3KNM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDQALQQML DRSCWVCFAT DEDDRTAEWV RPCRCRGSTK WVHQACLQRW
60 70 80 90 100
VDEKQRGNST ARVACPQCNA EYLIVFPKLG PVVYVLDLAD RLISKACPFA
110 120 130 140 150
AAGIMVGSIY WTAVTYGAVT VMQVVGHKEG LDVMERADPL FLLIGLPTIP
160 170 180 190 200
VMLILGKMIR WEDYVLRLWR KYSNKLQILN SIFPGIGCPV PRIPAEANPL
210 220 230 240 250
ADHVSATRIL CGALVFPTIA TIVGKLMFSS VNSNLQRTIL GGIAFVAIKG
260 270
AFKVYFKQQQ YLRQAHRKIL NYPEQEEA
Length:278
Mass (Da):31,232
Last modified:November 8, 2005 - v1
Checksum:iCB00A408E228A9EE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85V → A in AAH64752 (PubMed:15489334).Curated1
Sequence conflicti241 – 244GGIA → VRWI in BAB26154 (PubMed:16141072).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003478 mRNA. Translation: BAB22809.1.
AK009232 mRNA. Translation: BAB26154.1.
BC064752 mRNA. Translation: AAH64752.1.
BC107215 mRNA. Translation: AAI07216.1.
CCDSiCCDS29776.1.
RefSeqiNP_001157808.1. NM_001164336.1.
NP_081590.3. NM_027314.3.
UniGeneiMm.175989.

Genome annotation databases

EnsembliENSMUST00000024078; ENSMUSP00000024078; ENSMUSG00000023307.
ENSMUST00000215066; ENSMUSP00000150553; ENSMUSG00000111123.
GeneIDi69104.
KEGGimmu:69104.
UCSCiuc008hif.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMARH5_MOUSE
AccessioniPrimary (citable) accession number: Q3KNM2
Secondary accession number(s): Q3KNM3
, Q6P230, Q9CPS3, Q9CTI6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 8, 2005
Last modified: May 10, 2017
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways