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Protein

NADH-cytochrome b5 reductase 2

Gene

Cyb5r2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1) (By similarity).By similarity

Catalytic activityi

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactori

FADBy similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi107 – 137FADBy similarityAdd BLAST31
Nucleotide bindingi146 – 181FADBy similarityAdd BLAST36

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandFAD, Flavoprotein, NAD

Enzyme and pathway databases

ReactomeiR-MMU-1237044 Erythrocytes take up carbon dioxide and release oxygen

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-cytochrome b5 reductase 2 (EC:1.6.2.2)
Short name:
b5R.2
Gene namesi
Name:Cyb5r2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2444415 Cyb5r2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002875491 – 276NADH-cytochrome b5 reductase 2Add BLAST276

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17N6-acetyllysineBy similarity1
Modified residuei18PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ3KNK3
PeptideAtlasiQ3KNK3
PRIDEiQ3KNK3

PTM databases

iPTMnetiQ3KNK3
PhosphoSitePlusiQ3KNK3

Expressioni

Gene expression databases

BgeeiENSMUSG00000048065
CleanExiMM_CYB5R2
GenevisibleiQ3KNK3 MM

Structurei

3D structure databases

ProteinModelPortaliQ3KNK3
SMRiQ3KNK3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 127FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000008881
HOGENOMiHOG000175005
HOVERGENiHBG052580
InParanoidiQ3KNK3
KOiK00326
OMAiIRHITKN
OrthoDBiEOG091G0EIS
PhylomeDBiQ3KNK3
TreeFamiTF314333

Family and domain databases

InterProiView protein in InterPro
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR001834 NADH-Cyt_B5_reductase
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00371 FPNCR
SUPFAMiSSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3KNK3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVKKKDLIT LQDPEAKYPL PLIEKEQISH NTRRFRFGLP SPDHVLGLPV
60 70 80 90 100
GNYVHLLAQI NNELVIRAYT PVSSDDDQGF VDLIIKIYFK NVHPKYPEGG
110 120 130 140 150
KMTQYLENMK IGDTILFRGP TGRLFYNEPG TLLIKANKTS EPEKKLVHHL
160 170 180 190 200
GMIAGGTGIT PMLQLIRHIT KDTSDETRMS LLFANQTEED ILLRKELEEV
210 220 230 240 250
ATTHHKQFNL WYTLDRPPSD WKYSSGFVSA DMIKEHLPPP GEDTLILVCG
260 270
PPPLIQAAAH PSLEQLSYTK DMIFIY
Length:276
Mass (Da):31,360
Last modified:May 15, 2007 - v2
Checksum:i56596AF2F33F33E3
GO
Isoform 2 (identifier: Q3KNK3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     130-130: G → GCQTRAAEVKNIFIFLG

Note: No experimental confirmation available.
Show »
Length:292
Mass (Da):33,152
Checksum:i1C4641E3F9297E31
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti132L → I in AAI07239 (PubMed:15489334).Curated1
Sequence conflicti267S → G in AAI07239 (PubMed:15489334).Curated1
Sequence conflicti275I → T in AAI07239 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025560130G → GCQTRAAEVKNIFIFLG in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK085272 mRNA Translation: BAC39408.1
AK147952 mRNA Translation: BAE28247.1
BC107238 mRNA Translation: AAI07239.1
BC107239 mRNA Translation: AAI07240.1
CCDSiCCDS57579.1 [Q3KNK3-2]
CCDS85373.1 [Q3KNK3-1]
RefSeqiNP_001192156.1, NM_001205227.1 [Q3KNK3-2]
NP_796190.1, NM_177216.4 [Q3KNK3-1]
XP_017177793.1, XM_017322304.1 [Q3KNK3-1]
XP_017177794.1, XM_017322305.1 [Q3KNK3-1]
UniGeneiMm.132828

Genome annotation databases

EnsembliENSMUST00000052438; ENSMUSP00000050061; ENSMUSG00000048065 [Q3KNK3-2]
ENSMUST00000208217; ENSMUSP00000146504; ENSMUSG00000048065 [Q3KNK3-1]
GeneIDi320635
KEGGimmu:320635
UCSCiuc009jbi.2 mouse [Q3KNK3-1]
uc012frx.2 mouse [Q3KNK3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNB5R2_MOUSE
AccessioniPrimary (citable) accession number: Q3KNK3
Secondary accession number(s): Q3UGG1, Q8BUG7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: May 23, 2018
This is version 91 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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