Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADH-cytochrome b5 reductase 2

Gene

Cyb5r2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1) (By similarity).By similarity

Catalytic activityi

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactori

FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi107 – 13731FADBy similarityAdd
BLAST
Nucleotide bindingi146 – 18136FADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

ReactomeiR-MMU-1237044. Erythrocytes take up carbon dioxide and release oxygen.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-cytochrome b5 reductase 2 (EC:1.6.2.2)
Short name:
b5R.2
Gene namesi
Name:Cyb5r2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2444415. Cyb5r2.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: MGI
  • mitochondrion Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276NADH-cytochrome b5 reductase 2PRO_0000287549Add
BLAST

Proteomic databases

MaxQBiQ3KNK3.
PeptideAtlasiQ3KNK3.
PRIDEiQ3KNK3.

PTM databases

iPTMnetiQ3KNK3.
PhosphoSiteiQ3KNK3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000048065.
CleanExiMM_CYB5R2.
GenevisibleiQ3KNK3. MM.

Structurei

3D structure databases

ProteinModelPortaliQ3KNK3.
SMRiQ3KNK3. Positions 9-276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 127113FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000008881.
HOGENOMiHOG000175005.
HOVERGENiHBG052580.
InParanoidiQ3KNK3.
KOiK00326.
OMAiEEITHNT.
OrthoDBiEOG091G0EIS.
PhylomeDBiQ3KNK3.
TreeFamiTF314333.

Family and domain databases

InterProiIPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR001834. NADH-Cyt_B5_reductase.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00406. CYTB5RDTASE.
PR00371. FPNCR.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3KNK3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVKKKDLIT LQDPEAKYPL PLIEKEQISH NTRRFRFGLP SPDHVLGLPV
60 70 80 90 100
GNYVHLLAQI NNELVIRAYT PVSSDDDQGF VDLIIKIYFK NVHPKYPEGG
110 120 130 140 150
KMTQYLENMK IGDTILFRGP TGRLFYNEPG TLLIKANKTS EPEKKLVHHL
160 170 180 190 200
GMIAGGTGIT PMLQLIRHIT KDTSDETRMS LLFANQTEED ILLRKELEEV
210 220 230 240 250
ATTHHKQFNL WYTLDRPPSD WKYSSGFVSA DMIKEHLPPP GEDTLILVCG
260 270
PPPLIQAAAH PSLEQLSYTK DMIFIY
Length:276
Mass (Da):31,360
Last modified:May 15, 2007 - v2
Checksum:i56596AF2F33F33E3
GO
Isoform 2 (identifier: Q3KNK3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     130-130: G → GCQTRAAEVKNIFIFLG

Note: No experimental confirmation available.
Show »
Length:292
Mass (Da):33,152
Checksum:i1C4641E3F9297E31
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti132 – 1321L → I in AAI07239 (PubMed:15489334).Curated
Sequence conflicti267 – 2671S → G in AAI07239 (PubMed:15489334).Curated
Sequence conflicti275 – 2751I → T in AAI07239 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei130 – 1301G → GCQTRAAEVKNIFIFLG in isoform 2. 1 PublicationVSP_025560

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK085272 mRNA. Translation: BAC39408.1.
AK147952 mRNA. Translation: BAE28247.1.
BC107238 mRNA. Translation: AAI07239.1.
BC107239 mRNA. Translation: AAI07240.1.
CCDSiCCDS57579.1. [Q3KNK3-2]
RefSeqiNP_001192156.1. NM_001205227.1. [Q3KNK3-2]
NP_796190.1. NM_177216.4. [Q3KNK3-1]
UniGeneiMm.132828.

Genome annotation databases

EnsembliENSMUST00000052438; ENSMUSP00000050061; ENSMUSG00000048065. [Q3KNK3-2]
ENSMUST00000208217; ENSMUSP00000146504; ENSMUSG00000048065. [Q3KNK3-1]
GeneIDi320635.
KEGGimmu:320635.
UCSCiuc009jbi.2. mouse. [Q3KNK3-1]
uc012frx.2. mouse. [Q3KNK3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK085272 mRNA. Translation: BAC39408.1.
AK147952 mRNA. Translation: BAE28247.1.
BC107238 mRNA. Translation: AAI07239.1.
BC107239 mRNA. Translation: AAI07240.1.
CCDSiCCDS57579.1. [Q3KNK3-2]
RefSeqiNP_001192156.1. NM_001205227.1. [Q3KNK3-2]
NP_796190.1. NM_177216.4. [Q3KNK3-1]
UniGeneiMm.132828.

3D structure databases

ProteinModelPortaliQ3KNK3.
SMRiQ3KNK3. Positions 9-276.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ3KNK3.
PhosphoSiteiQ3KNK3.

Proteomic databases

MaxQBiQ3KNK3.
PeptideAtlasiQ3KNK3.
PRIDEiQ3KNK3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052438; ENSMUSP00000050061; ENSMUSG00000048065. [Q3KNK3-2]
ENSMUST00000208217; ENSMUSP00000146504; ENSMUSG00000048065. [Q3KNK3-1]
GeneIDi320635.
KEGGimmu:320635.
UCSCiuc009jbi.2. mouse. [Q3KNK3-1]
uc012frx.2. mouse. [Q3KNK3-2]

Organism-specific databases

CTDi51700.
MGIiMGI:2444415. Cyb5r2.

Phylogenomic databases

GeneTreeiENSGT00390000008881.
HOGENOMiHOG000175005.
HOVERGENiHBG052580.
InParanoidiQ3KNK3.
KOiK00326.
OMAiEEITHNT.
OrthoDBiEOG091G0EIS.
PhylomeDBiQ3KNK3.
TreeFamiTF314333.

Enzyme and pathway databases

ReactomeiR-MMU-1237044. Erythrocytes take up carbon dioxide and release oxygen.

Miscellaneous databases

PROiQ3KNK3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000048065.
CleanExiMM_CYB5R2.
GenevisibleiQ3KNK3. MM.

Family and domain databases

InterProiIPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR001834. NADH-Cyt_B5_reductase.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00406. CYTB5RDTASE.
PR00371. FPNCR.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNB5R2_MOUSE
AccessioniPrimary (citable) accession number: Q3KNK3
Secondary accession number(s): Q3UGG1, Q8BUG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: September 7, 2016
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.