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Protein

Mas-related G-protein coupled receptor member B2

Gene

Mrgprb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core. Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction (PubMed:25517090).1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Mas-related G-protein coupled receptor member B2
Gene namesi
Name:Mrgprb2
Synonyms:Mrgb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2441674. Mrgprb2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 40ExtracellularSequence analysisAdd BLAST40
Transmembranei41 – 61Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini62 – 89CytoplasmicSequence analysisAdd BLAST28
Transmembranei90 – 110Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini111ExtracellularSequence analysis1
Transmembranei112 – 132Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini133 – 157CytoplasmicSequence analysisAdd BLAST25
Transmembranei158 – 178Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini179 – 191ExtracellularSequence analysisAdd BLAST13
Transmembranei192 – 212Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini213 – 231CytoplasmicSequence analysisAdd BLAST19
Transmembranei232 – 252Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini253 – 268ExtracellularSequence analysisAdd BLAST16
Transmembranei269 – 289Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini290 – 338CytoplasmicSequence analysisAdd BLAST49

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype in normal conditions. Absence of pseudo-allergic reactions in response to small-molecule therapeutic drugs: secretagogue-induced histamine release, inflammation and airway contraction are abolished.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003048781 – 338Mas-related G-protein coupled receptor member B2Add BLAST338

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi9N-linked (GlcNAc...)Sequence analysis1
Glycosylationi18N-linked (GlcNAc...)Sequence analysis1
Glycosylationi23N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ3KNA1.
PRIDEiQ3KNA1.

PTM databases

iPTMnetiQ3KNA1.
PhosphoSitePlusiQ3KNA1.

Expressioni

Tissue specificityi

Mast cell-specific.1 Publication

Gene expression databases

BgeeiENSMUSG00000050425.
CleanExiMM_MRGPRB2.
ExpressionAtlasiQ3KNA1. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000061878.

Structurei

3D structure databases

ProteinModelPortaliQ3KNA1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Mas subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J9NM. Eukaryota.
ENOG4111AUB. LUCA.
GeneTreeiENSGT00550000074531.
HOGENOMiHOG000015412.
HOVERGENiHBG003592.
InParanoidiQ3KNA1.
KOiK08396.
OrthoDBiEOG091G0CSX.
PhylomeDBiQ3KNA1.
TreeFamiTF336336.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR026234. MRGPCRFAMILY.
[Graphical view]
PANTHERiPTHR11334. PTHR11334. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR02108. MRGPCRFAMILY.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3KNA1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGDFLIKNL STSAWKTNIT VLNGSYYIDT SVCVTRNQAM ILLSIIISLV
60 70 80 90 100
GMGLNAIVLW FLGIRMHTNA FTVYILNLAM ADFLYLCSQF VICLLIAFYI
110 120 130 140 150
FYSIDINIPL VLYVVPIFAY LSGLSILSTI SIERCLSVIW PIWYRCKRPR
160 170 180 190 200
HTSAITCFVL WVMSLLLGLL EGKACGLLFN SFDSYWCETF DVITNIWSVV
210 220 230 240 250
FFGVLCGSSL TLLVRIFCGS QRIPMTRLYV TITLTVLVFL IFGLPFGIYW
260 270 280 290 300
ILYQWISNFY YVEICNFYLE ILFLSCVNSC MNPIIYFLVG SIRHRRFRRK
310 320 330
TLKLLLQRAM QDTPEEEQSG NKSSSEHPEE LETVQSCS
Length:338
Mass (Da):38,832
Last modified:September 11, 2007 - v2
Checksum:i1B0A091D67C868B9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28I → F in BAC26422 (PubMed:16141072).Curated1
Sequence conflicti263E → K in AAI07391 (PubMed:15489334).Curated1
Sequence conflicti263E → K in AAI07392 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY042200 Genomic DNA. Translation: AAK91796.1.
AK029369 mRNA. Translation: BAC26422.1.
BC107390 mRNA. Translation: AAI07391.1.
BC107391 mRNA. Translation: AAI07392.1.
CCDSiCCDS21303.1.
RefSeqiNP_780740.2. NM_175531.4.
UniGeneiMm.83689.

Genome annotation databases

EnsembliENSMUST00000052730; ENSMUSP00000061878; ENSMUSG00000050425.
GeneIDi243979.
KEGGimmu:243979.
UCSCiuc009hat.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY042200 Genomic DNA. Translation: AAK91796.1.
AK029369 mRNA. Translation: BAC26422.1.
BC107390 mRNA. Translation: AAI07391.1.
BC107391 mRNA. Translation: AAI07392.1.
CCDSiCCDS21303.1.
RefSeqiNP_780740.2. NM_175531.4.
UniGeneiMm.83689.

3D structure databases

ProteinModelPortaliQ3KNA1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000061878.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ3KNA1.
PhosphoSitePlusiQ3KNA1.

Proteomic databases

PaxDbiQ3KNA1.
PRIDEiQ3KNA1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052730; ENSMUSP00000061878; ENSMUSG00000050425.
GeneIDi243979.
KEGGimmu:243979.
UCSCiuc009hat.2. mouse.

Organism-specific databases

CTDi243979.
MGIiMGI:2441674. Mrgprb2.

Phylogenomic databases

eggNOGiENOG410J9NM. Eukaryota.
ENOG4111AUB. LUCA.
GeneTreeiENSGT00550000074531.
HOGENOMiHOG000015412.
HOVERGENiHBG003592.
InParanoidiQ3KNA1.
KOiK08396.
OrthoDBiEOG091G0CSX.
PhylomeDBiQ3KNA1.
TreeFamiTF336336.

Miscellaneous databases

PROiQ3KNA1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050425.
CleanExiMM_MRGPRB2.
ExpressionAtlasiQ3KNA1. baseline and differential.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR026234. MRGPCRFAMILY.
[Graphical view]
PANTHERiPTHR11334. PTHR11334. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR02108. MRGPCRFAMILY.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMRGB2_MOUSE
AccessioniPrimary (citable) accession number: Q3KNA1
Secondary accession number(s): Q8CDY4, Q91ZC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: November 2, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

In spite of its official gene name, this protein may be the functional ortholog of human MRGPRX2, in terms of expression pattern and pharmacology.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.