Q3KN18 (GATB_CHLTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Short name=Asp/Glu-ADT subunit B
EC=6.3.5.-

Gene names

Name:	gatB
Ordered Locus Names:	CTA_0005

Organism

Chlamydia trachomatis serovar A (strain HAR-13 / ATCC VR-571B) [Complete proteome] [HAMAP]

Taxonomic identifier

315277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chlamydiae/Verrucomicrobia group › Chlamydiae › Chlamydiia › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia › Chlamydia trachomatis

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00121
Catalytic activity	ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00121 ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate. HAMAP-Rule MF_00121
Subunit structure	Heterotrimer of A, B and C subunits By similarity. HAMAP-Rule MF_00121
Sequence similarities	Belongs to the GatB/GatE family. GatB subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B HAMAP-Rule MF_00121		PRO_0000241208

Sequences

	Sequence		Length	Mass (Da)
	Q3KN18 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 7E2014461830698E	FASTA	488	55,048
	10 20 30 40 50 60 MGIAHTEWES VIGLEVHVEL NTESKLFSPA RNHFGDEPNT NISPVCTGMP GSLPVLNKDA 70 80 90 100 110 120 VRKAVLFGCA VEGDVALFSR FDRKSYFYPD SPRNFQITQY EHPIVRGGCI RAVVEGEEKT 130 140 150 160 170 180 FELAQTHLED DAGMLKHFGD FAGVDYNRAG VPLIEIVSKP CMFSAEDAVA YANALVSILG 190 200 210 220 230 240 YIGISDCNME EGSIRFDVNI SVRPRGSREL RNKVEIKNMN SFTFMAQALE AEKRRQIEEY 250 260 270 280 290 300 LSYPNEDPKK VVPAATYRWD PEKKKTVLMR LKERAEDYMY FVEPDLPVLQ ITETYIDEVR 310 320 330 340 350 360 QTLPELPHSK YMRYITDFDI AEDLAMILVS DRHTAHFFET ATMSCKNYRA LSNWITVEFA 370 380 390 400 410 420 GRCKARGKTL PFTGILPEWV AQLVNFIDRG VITGKIAKEI ADRMVSSFGE SPEDILRRHP 430 440 450 460 470 480 SLLPMTDDHA LRAIVKEVVA QNTASVADYK NGKAKALGFL VGQIMKRTEG KAPPKRVNEL LLAAMRDM

References

[1]

"Comparative genomic analysis of Chlamydia trachomatis oculotropic and genitotropic strains." Carlson J.H., Porcella S.F., McClarty G., Caldwell H.D. Infect. Immun. 73:6407-6418(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HAR-13 / ATCC VR-571B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000051 Genomic DNA. Translation: AAX50254.1.
RefSeq	YP_327802.1. NC_007429.1.
3D structure databases
ProteinModelPortal	Q3KN18.
ModBase	Search...
Protein-protein interaction databases
STRING	315277.CTA_0005.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAX50254; AAX50254; CTA_0005.
GeneID	3688099.
KEGG	cta:CTA_0005.
PATRIC	32021852. VBIChlTra31516_0007.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0064.
HOGENOM	HOG000223742.
KO	K02434.
OMA	KNYFYAD.
ProtClustDB	PRK05477.
Enzyme and pathway databases
BioCyc	CTRA315277:GI4C-5-MONOMER.
Family and domain databases
Gene3D	1.10.10.410. 1 hit.
HAMAP	MF_00121. GatB.
InterPro	IPR004413. Apn/Gln-ADT_bsu. IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E. IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat. IPR018027. Asn/Gln_amidotransferase. IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel. IPR023168. GatB_Yqey_C. IPR017958. Gln-tRNA_amidoTrfase_suB_CS. [Graphical view]
PANTHER	PTHR11659. PTHR11659. 1 hit.
Pfam	PF02934. GatB_N. 1 hit. PF02637. GatB_Yqey. 1 hit. [Graphical view]
SMART	SM00845. GatB_Yqey. 1 hit. [Graphical view]
SUPFAM	SSF89095. GatB_Yqey. 1 hit.
TIGRFAMs	TIGR00133. gatB. 1 hit.
PROSITE	PS01234. GATB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GATB_CHLTA

Accession

Primary (citable) accession number: Q3KN18

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	November 8, 2005
Last modified:	May 29, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program