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Q3KN03

- SYI_CHLTA

UniProt

Q3KN03 - SYI_CHLTA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydia trachomatis serovar A (strain HAR-13 / ATCC VR-571B)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei592 – 5921ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCTRA315277:GI4C-21-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CTA_0021
OrganismiChlamydia trachomatis serovar A (strain HAR-13 / ATCC VR-571B)
Taxonomic identifieri315277 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000002532: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Isoleucine--tRNA ligasePRO_1000216254Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi315277.CTA_0021.

Structurei

3D structure databases

ProteinModelPortaliQ3KN03.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 5611"HIGH" regionAdd
BLAST
Motifi589 – 5935"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKWIISEI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3KN03-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDNEDKISIS AKEEKILSFW KEQDIFQKTL DNREGCPTFS FYDGPPFATG
60 70 80 90 100
LPHYGHLLAG TIKDVVCRYA SMDGHYVPRR FGWDCHGVPV EYEVEKSLGL
110 120 130 140 150
TEPGAIERFG VANFNEECRK IVFRYADEWK YFVDRIGRWV DFSATWRTMD
160 170 180 190 200
LSFMESVWWV FRSLYDQGLV YEGTKVVPFS TKLGTPLSNF EAGQNYKEVD
210 220 230 240 250
DPSVVIKFAL QDNQGFLLAW TTTPWTLVSN MALAVHPELT YVRIKDKESG
260 270 280 290 300
DEYILGQESL PRWFPDRESY EWIGQLSGKS LVGQSYEPLF PYFQDKKELE
310 320 330 340 350
AFRILPADFI EESEGTGIVH MAPAFGEADF FACQEHNVPL VCPVDNQGCY
360 370 380 390 400
TAEVKDFVGE YIKSADKGIA RRLKNENKLF YQGTVRHRYP FCWRTDSPLI
410 420 430 440 450
YKAVNSWFVA VEKVKSKMLK ANESIHWTPG HIKQGRFGKW LEGARDWAIS
460 470 480 490 500
RNRYWGTPIP IWRSDDGELL VIGSIQELEA LSGQKIVDLH RHFIDEIEIN
510 520 530 540 550
QNGKSFRRIP YVFDCWFDSG AMPYAQNHYP FERAEETEAC FPADFIAEGL
560 570 580 590 600
DQTRGWFYTL TVIAAALFDQ PAFKNVIVNG IILAEDGNKM SKRLNNYPSP
610 620 630 640 650
KMIMDAYGAD ALRLYLLNSV VVKAEDLRFS DKGVESVLKQ VLLPLSNALA
660 670 680 690 700
FYKTYAELYG FDPKETDNIE LAEIDCWILS SLYSLVGKTR ESMSQYDLHA
710 720 730 740 750
AVNPFVDFIE DLTNWYIRRS RRRFWDAEDS ADRRAAFSTL YEVLVVFSKV
760 770 780 790 800
IAPFIPFIAE DMYQQLRGET DPESVHLCDF PHVVLEKILP DLERKMQDIR
810 820 830 840 850
EIVALGHSLR KEHKLKVRQP LQNVYIVGSK ERMEALAQVG SLIGEELNVK
860 870 880 890 900
DVHFCSETPE YVTTLIKPNF RTLGKKVGNR LPEIQRALAR LPQEQIRAFM
910 920 930 940 950
HKGQMVVSLG EETISLDKED ITVSWASAEG FVARSSASFV AVLDCQLTEP
960 970 980 990 1000
LIMEGIAREL VNKINTMRRN GKLHVSDRIA IRLHAPVIVQ EAFALHKEYI
1010 1020 1030
CEETLTTSVS VIDYKEGEEW DINGHAVSFV LERVER
Length:1,036
Mass (Da):118,905
Last modified:November 8, 2005 - v1
Checksum:i81330357B394C2AC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000051 Genomic DNA. Translation: AAX50269.1.
RefSeqiWP_011324523.1. NC_007429.1.
YP_327817.1. NC_007429.1.

Genome annotation databases

EnsemblBacteriaiAAX50269; AAX50269; CTA_0021.
GeneIDi3688115.
KEGGicta:CTA_0021.
PATRICi32021892. VBIChlTra31516_0027.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000051 Genomic DNA. Translation: AAX50269.1 .
RefSeqi WP_011324523.1. NC_007429.1.
YP_327817.1. NC_007429.1.

3D structure databases

ProteinModelPortali Q3KN03.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 315277.CTA_0021.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAX50269 ; AAX50269 ; CTA_0021 .
GeneIDi 3688115.
KEGGi cta:CTA_0021.
PATRICi 32021892. VBIChlTra31516_0027.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KWIISEI.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CTRA315277:GI4C-21-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative genomic analysis of Chlamydia trachomatis oculotropic and genitotropic strains."
    Carlson J.H., Porcella S.F., McClarty G., Caldwell H.D.
    Infect. Immun. 73:6407-6418(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HAR-13 / ATCC VR-571B.

Entry informationi

Entry nameiSYI_CHLTA
AccessioniPrimary (citable) accession number: Q3KN03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: November 8, 2005
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3