Q3KMH9 (PYRG_CHLTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CTP synthase
EC=6.3.4.2

Alternative name(s):
CTP synthetase
UTP--ammonia ligase

Gene names

Name:	pyrG
Ordered Locus Names:	CTA_0201

Organism

Chlamydia trachomatis serovar A (strain HAR-13 / ATCC VR-571B) [Complete proteome] [HAMAP]

Taxonomic identifier

315277 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity. HAMAP MF_01227
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity. HAMAP MF_01227
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP MF_01227
Subunit structure	Homotetramer By similarity. HAMAP MF_01227
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 539	539	CTP synthase HAMAP MF_01227		PRO_0000266090
Regions
	Domain	294 – 532	239	Glutamine amidotransferase type-1
	Region	1 – 255	255	Aminator domain HAMAP MF_01227
Sites
	Active site	380	1	Nucleophile By similarity
	Active site	505	1	By similarity
	Active site	507	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3KMH9 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 413F7475BCDDD7CE	FASTA	539	60,204
	10 20 30 40 50 60 MSFKSIFLTG GVVSSLGKGL TAASLALLLE RQDLKVAMLK LDPYLNVDPG TMNPYEHGEV 70 80 90 100 110 120 YVTDDGVETD LDLGHYHRFS SVQLSKYSTA TSGQIYTKVL TKERNGEFLG STVQVIPHVT 130 140 150 160 170 180 NEIINVIQSC ADHHKPDILI VEIGGTIGDI ESLPFLEAVR QFRCEHPQDC LSIHMTYVPY 190 200 210 220 230 240 LRAAKEIKTK PTQHSVQNLR SIGISPDVIL CRSEAPLSTE VKRKISLFCN VPEHAVFNAI 250 260 270 280 290 300 DLERSIYEMP LLLAKENISD FLLNKLGFSP KPLDLSDWQD LVEALCDKER QHVRIGLVGK 310 320 330 340 350 360 YLEHEDAYKS VFESLFHASV PANCSLELVP IAPESEDLLE QLSQCDGCLI PGGFGTRSWE 370 380 390 400 410 420 GKISAARYCR ERNIPCFGIC LGMQALVVEY ARNVLDKPLA NSMEINPETP DPVVCMMEGQ 430 440 450 460 470 480 DSVVKGGTMR LGAYPCRIAP GSLASAAYKT DLVQERHRHR YEVNPSYIER LEEHGLKIAG 490 500 510 520 530 VCPLGELCEI VEIPNHRWML GVQFHPEFLS KLAKPHPLFI EFIRAAKAYS LEKANHEHR

References

[1]

"Comparative genomic analysis of Chlamydia trachomatis oculotropic and genitotropic strains." Carlson J.H., Porcella S.F., McClarty G., Caldwell H.D. Infect. Immun. 73:6407-6418(2005) [PubMed: 16177312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HAR-13 / ATCC VR-571B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000051 Genomic DNA. Translation: AAX50443.1.
RefSeq	YP_327991.1. NC_007429.1.
3D structure databases
HSSP	HSSP built from PDB template 2C5M based on UniProtKB P17812.
ProteinModelPortal	Q3KMH9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3KMH9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3687227.
GenomeReviews	Gene locus CTA_0201 in contig CP000051_GR.
KEGG	cta:CTA_0201.
PATRIC	32022268. VBIChlTra31516_0208.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0504.
HOGENOM	HBG597806.
OMA	CRAERNI.
PhylomeDB	Q3KMH9.
ProtClustDB	PRK05380.
Family and domain databases
HAMAP	MF_01227. PyrG. [Tree]
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. [Graphical view]
KO	K01937.
PANTHER	PTHR11550. PyrG_synth. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_CHLTA

Accession

Primary (citable) accession number: Q3KMH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program