Reviewed, UniProtKB/Swiss-Prot Q3KMG9 (TGT_CHLTA)

Names and origin

Protein names

Recommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme

Gene names

Name:	tgt
Ordered Locus Names:	CTA_0211

Organism

Chlamydia trachomatis (strain A/HAR-13 / ATCC VR-571B) [Complete proteome] [HAMAP]

Taxonomic identifier

315277 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_1000016775
Sites
	Active site	89	1	Nucleophile By similarity
	Metal binding	319	1	Zinc By similarity
	Metal binding	321	1	Zinc By similarity
	Metal binding	324	1	Zinc By similarity
	Metal binding	351	1	Zinc By similarity
	Binding site	90	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3KMG9-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: B4F7FF3B79D294DB	FASTA	372	40,932
	10 20 30 40 50 60 MALRFEILHQ SKKSRARVGR IETAHGYIDT PAFVPVATNG ALKGVLDHSN IPLMFCNTYH 70 80 90 100 110 120 LIVHPGAEAI AAMGGLHQFI GRNAPIITDS GGFQIFSLAY GSVAEEIKSC GKKKGGNTII 130 140 150 160 170 180 KVNDDGVHFK SYRDGRKLFL SPEISVQAQK DLGADIILPL DELLPFHADP TYFHQSSQRT 190 200 210 220 230 240 YVWEKRSLDY HLKNPGIQSM YGVIHGGTFP DQRKLGCKFV EDLPFDGSAI GGSLGKNLQD 250 260 270 280 290 300 IVEVVGVTAA NLSAERPRHL LGIGDLPSIW ATVGFGIDSF DSSYPTKAAR HGMILTLQGP 310 320 330 340 350 360 LKINNQRYSS DLNPIEPGCS CLACSQGITR AYLRHLFKVH EPNAGIWASI HNMHHMQKVM 370 REIREGILND RI

References

[1]	"Comparative genomic analysis of Chlamydia trachomatis oculotropic and genitotropic strains." Carlson J.H., Porcella S.F., McClarty G., Caldwell H.D. Infect. Immun. 73:6407-6418(2005) [PubMed: 16177312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000051 Genomic DNA. Translation: AAX50453.1.
RefSeq	YP_328001.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3687237.
GenomeReviews	Gene locus CTA_0211 in contig CP000051_GR.
KEGG	cta:CTA_0211.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3KMG9.
OMA	Q3KMG9. MGVGLPE.
Enzyme and pathway databases
BioCyc	CTRA315277-1:CTA_0211-MON.
Family and domain databases
HAMAP	MF_00168. [Tree]
InterPro	IPR004803. QtRNA_ribo_trans. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_CHLTA

Accession

Primary (citable) accession number: Q3KMG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)