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Protein

3-deoxy-D-manno-octulosonic acid transferase

Gene

waaA

Organism
Chlamydia trachomatis serovar A (strain ATCC VR-571B / DSM 19440 / HAR-13)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of three 3-deoxy-D-manno-octulosonate (Kdo) residues from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A. Thus generates the genus-specific LPS epitope of Chlamydia, composed of the trisaccharide alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo.By similarity

Catalytic activityi

Lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->6)-lipid IV(A) + CMP.
Alpha-Kdo-(2->6)-lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP.
Alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP-beta-Kdo = alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IV(A) + CMP.

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei67Proton acceptorBy similarity1
Sitei138Transition state stabilizerBy similarity1
Sitei216Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processLipopolysaccharide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00958.

Protein family/group databases

CAZyiGT30. Glycosyltransferase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
3-deoxy-D-manno-octulosonic acid transferase (EC:2.4.99.12, EC:2.4.99.13, EC:2.4.99.14)
Short name:
Kdo transferase
Alternative name(s):
Kdo(2)-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Kdo-lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase
Gene namesi
Name:waaA
Synonyms:gseA, kdtA
Ordered Locus Names:CTA_0228
OrganismiChlamydia trachomatis serovar A (strain ATCC VR-571B / DSM 19440 / HAR-13)
Taxonomic identifieri315277 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000002532 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei5 – 27Helical; Signal-anchorSequence analysisAdd BLAST23

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802851 – 4313-deoxy-D-manno-octulosonic acid transferaseAdd BLAST431

Structurei

3D structure databases

ProteinModelPortaliQ3KMF4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni275 – 276CMP-Kdo bindingBy similarity2
Regioni315 – 317CMP-Kdo bindingBy similarity3
Regioni342 – 345CMP-Kdo bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000035072.
KOiK02527.
OMAiFIKYEFW.

Family and domain databases

InterProiView protein in InterPro
IPR007507. Glycos_transf_N.
PfamiView protein in Pfam
PF04413. Glycos_transf_N. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3KMF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRRWLTSRL YDAFLVCAFF VSAPRIFYKV FFHGKYIDSW KIRFGVQKPF
60 70 80 90 100
VKGEGPLVWF HGASVGEVSL LAPLLNRWRE EFPEWRFVVT TCSEAGVHTA
110 120 130 140 150
RRLYESLGAT VFVLPLDLSC IIKSVVRKLA PDIVIFSEGD CWLHFLTESK
160 170 180 190 200
RLGAKAFLIN GKLSEHSCKR FSFLKRLGRN YFAPLDLLIL QDELYKQRFM
210 220 230 240 250
QIGISSDKIH VTGNMKTFIE SSLATNRRDF WRAKLQISSQ DRLIVLGSVH
260 270 280 290 300
PKDVEVWAEV VSHFHNSSTK ILWVPRHLEK LKEHAKLLEK AGILFGLWSQ
310 320 330 340 350
GASFRQYNSL IMDAMGVLKD IYSAADIAFV GGTFDPSVGG HNLLEPLQKE
360 370 380 390 400
VPLMFGPYIY SQSVLAEKLR EKEAGLSVNK ETLLDVVTDL LQNEKNRQAY
410 420 430
IEKGKSFLKQ EENSFQQTWE ILKSQITCMK I
Length:431
Mass (Da):49,465
Last modified:November 8, 2005 - v1
Checksum:i6691DA206B1B12A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22652 Genomic DNA. Translation: CAA80367.1.
CP000051 Genomic DNA. Translation: AAX50468.1.
PIRiI40894.
RefSeqiWP_011324640.1. NC_007429.1.

Genome annotation databases

EnsemblBacteriaiAAX50468; AAX50468; CTA_0228.
KEGGicta:CTA_0228.

Similar proteinsi

Entry informationi

Entry nameiKDTA_CHLTA
AccessioniPrimary (citable) accession number: Q3KMF4
Secondary accession number(s): Q46394
, Q46395, Q46396, Q46401, Q57440
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 8, 2005
Last modified: June 7, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families