Reviewed, UniProtKB/Swiss-Prot Q3KKT4 (MURD_CHLTA)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	CTA_0828

Organism

Chlamydia trachomatis (strain A/HAR-13 / ATCC VR-571B) [Complete proteome] [HAMAP]

Taxonomic identifier

315277 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000257176
Regions
	Nucleotide binding	108 – 114	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3KKT4-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 32A2BB047221AF5F	FASTA	416	46,113
	10 20 30 40 50 60 MGLERVVVIG LGVSGRSIAH FLAQKGVCVL GVDKSLHALQ NCPYIQEKYL ENEEFPSQVD 70 80 90 100 110 120 YVVRSPGVSK EHPWVQAAIA SHIPVMADIQ LAFQTEKFTE RESLAITGTT GKTTTILFLE 130 140 150 160 170 180 YLFKRSGIPA FAMGNVGIPI LDGMQNPGVR IVEISSFQLA DQEKSYPVLS GGMILNISDN 190 200 210 220 230 240 HLDYHGNFSE YFQAKQNLAL CMRNPDDLWV GDERFYGHLY LEEVQKYMRL LDKESALKPL 250 260 270 280 290 300 YLHDKYNYCC AYLLAKTEFP ISETSFIEAV ATFNKPPHRM EYLGQKQGIH YINDSKATTV 310 320 330 340 350 360 SATETALLGV GNQAIVILGG RNKGCTFSSL LPALRKAAKS VVAMGECAQE IARDLEEFPV 370 380 390 400 410 TVVKNLSEAL LCAEEQAVPG DVIVLSPACA SFDQFRSYEE RGAMFKQLVG MEEVLL

References

[1]	"Comparative genomic analysis of Chlamydia trachomatis oculotropic and genitotropic strains." Carlson J.H., Porcella S.F., McClarty G., Caldwell H.D. Infect. Immun. 73:6407-6418(2005) [PubMed: 16177312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000051 Genomic DNA. Translation: AAX51038.1.
RefSeq	YP_328586.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3687471.
GenomeReviews	Gene locus CTA_0828 in contig CP000051_GR.
KEGG	cta:CTA_0828.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3KKT4.
OMA	Q3KKT4. DSKATNF.
Enzyme and pathway databases
BioCyc	CTRA315277-1:CTA_0828-MON.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01266. DAO. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_CHLTA

Accession

Primary (citable) accession number: Q3KKT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)