Reviewed, UniProtKB/Swiss-Prot Q3KKL0 (TRMB_CHLTA)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
Alternative name(s):
    tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	CTA_0903

Organism

Chlamydia trachomatis (strain A/HAR-13 / ATCC VR-571B) [Complete proteome] [HAMAP]

Taxonomic identifier

315277 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057		PRO_0000288134
Sites
	Binding site	49	1	S-adenosyl-L-methionine By similarity
	Binding site	74	1	S-adenosyl-L-methionine By similarity
	Binding site	101	1	S-adenosyl-L-methionine By similarity
	Binding site	124	1	S-adenosyl-L-methionine By similarity
	Binding site	128	1	Substrate By similarity
	Binding site	160	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3KKL0-1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: 6A605C3022819F6A	FASTA	219	25,854
	10 20 30 40 50 60 MKLPYFWEER SPQIANHVFY VPNYYSRYEE FVMPTWQELF ANNGPICCEL CSGNGDWVVE 70 80 90 100 110 120 QALKDASVNW IAVEKRFDRV RKIWSKMGNY RVNNLLIVCG EAQTFFSHYV SDASFQKIVV 130 140 150 160 170 180 NFPDPWPKFR HRKHRLFQDL FVQDMMRTLV VGGQLTLATD DYNYLVNAIT VMLKYLSPGL 190 200 210 KSPHYINVKD NYGGSWFENL WRSKGQEIFC TEFIKRVGI

References

[1]	"Comparative genomic analysis of Chlamydia trachomatis oculotropic and genitotropic strains." Carlson J.H., Porcella S.F., McClarty G., Caldwell H.D. Infect. Immun. 73:6407-6418(2005) [PubMed: 16177312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000051 Genomic DNA. Translation: AAX51112.1. Different initiation.
RefSeq	YP_328660.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3688067.
GenomeReviews	Gene locus CTA_0903 in contig CP000051_GR.
KEGG	cta:CTA_0903.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3KKL0.
Enzyme and pathway databases
BioCyc	CTRA315277-1:CTA_0903-MON.
Family and domain databases
HAMAP	MF_01057. [Tree]
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
PANTHER	PTHR23417:SF1. Methyltransf_4. 1 hit.
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMB_CHLTA

Accession

Primary (citable) accession number: Q3KKL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	May 5, 2009
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)