Q3KJQ5 (GSH1_PSEPF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--cysteine ligase
EC=6.3.2.2

Alternative name(s):
Gamma-ECS
Short name=GCS
Gamma-glutamylcysteine synthetase

Gene names

Name:	gshA
Ordered Locus Names:	Pfl01_0257

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP MF_00578
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP MF_00578
Sequence similarities	Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	Glutamate--cysteine ligase HAMAP MF_00578		PRO_1000025182

Sequences

	Sequence		Length	Mass (Da)
	Q3KJQ5 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 4A96C284BB0BC5F6	FASTA	532	59,302
	10 20 30 40 50 60 MKESKLSELL NRRLALLGER ANLSLLEQCL HGIERECLRV TGEGRLAQTP HPEALGSALT 70 80 90 100 110 120 NEQITTDYSE SLLEFITPAL PDPADTLASL DKIHRFAYSK LGNEYLWSPS MPCPLPAEED 130 140 150 160 170 180 IPIAYYGTSN IGQLKYVYRK GLALRYGKTM QCIAGIHYNF SLPEKLWPLL KEAEGFVGTD 190 200 210 220 230 240 RDFQSSSYIA LIRNFRRYSW LLMYLFGASP ALDAGFLRGR AHQLEQLDPD TLYLPYATSL 250 260 270 280 290 300 RMSDLGYQSN AQAGLTPCYN DLASYTDSLR KAVATPYAPY VEVGTHQDGE WVQLNTNILQ 310 320 330 340 350 360 IENEYYSNIR PKRVTYTGER PIQALMARGI QYVEVRCLDI NPFLPMGIDL TESRFLDAFL 370 380 390 400 410 420 LYCALNESPL LTNNSCGNAT SNFLSVVKEG RRPGLQLQRD GQPVELKEWA AELLEKIAPL 430 440 450 460 470 480 AALLDQSHGG DAHSKALDAQ LAKVKDSSLT PSAQVLAAMA AHKESFAQFS LRQSQAHAEF 490 500 510 520 530 FRSEPLAAEE QAKFEELARS SLAQQAELEQ NEVGDFDVFV GSYQASILAI SN

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf0-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000094 Genomic DNA. Translation: ABA72001.1.
RefSeq	YP_345990.1. NC_007492.2.
3D structure databases
ProteinModelPortal	Q3KJQ5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3KJQ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3713244.
GenomeReviews	Gene locus Pfl01_0257 in contig CP000094_GR.
KEGG	pfo:Pfl01_0257.
PATRIC	19882532. VBIPseFlu44242_0255.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2918.
HOGENOM	HBG289023.
OMA	ADTLYLP.
ProtClustDB	PRK02107.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_0257-MONOMER.
Family and domain databases
HAMAP	MF_00578. Glu_cys_ligase. [Tree]
InterPro	IPR007370. Glu_cys_ligase. IPR006334. Glut_cys_ligase. [Graphical view]
KO	K01919.
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01434. Glu_cys_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_PSEPF

Accession

Primary (citable) accession number: Q3KJQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program