Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q3KJG6

- THII_PSEPF

UniProt

Q3KJG6 - THII_PSEPF

Protein

tRNA sulfurtransferase

Gene

thiI

Organism
Pseudomonas fluorescens (strain Pf0-1)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (08 Nov 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

    Catalytic activityi

    L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei267 – 2671ATPUniRule annotation
    Binding sitei289 – 2891ATP; via amide nitrogenUniRule annotation
    Binding sitei298 – 2981ATPUniRule annotation
    Active sitei457 – 4571Cysteine persulfide intermediateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi185 – 1862ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. sulfurtransferase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-HAMAP
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
    3. thiazole biosynthetic process Source: InterPro
    4. tRNA thio-modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Enzyme and pathway databases

    BioCyciPFLU205922:GJBD-354-MONOMER.
    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
    Alternative name(s):
    Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
    Thiamine biosynthesis protein ThiIUniRule annotation
    tRNA 4-thiouridine synthaseUniRule annotation
    Gene namesi
    Name:thiIUniRule annotation
    Ordered Locus Names:Pfl01_0346
    OrganismiPseudomonas fluorescens (strain Pf0-1)
    Taxonomic identifieri205922 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002704: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 484484tRNA sulfurtransferasePRO_1000074253Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi346 ↔ 457Redox-activeUniRule annotation

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi205922.Pfl01_0346.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3KJG6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini63 – 167105THUMPUniRule annotationAdd
    BLAST
    Domaini405 – 48379RhodaneseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ThiI family.UniRule annotation
    Contains 1 rhodanese domain.UniRule annotation
    Contains 1 THUMP domain.UniRule annotation

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    eggNOGiCOG0301.
    HOGENOMiHOG000227469.
    KOiK03151.
    OMAiKLFPEIM.
    OrthoDBiEOG6TBHGR.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPiMF_00021. ThiI.
    InterProiIPR001763. Rhodanese-like_dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR026340. Thiazole_biosynth_dom.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view]
    PfamiPF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view]
    SMARTiSM00981. THUMP. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    TIGRFAMsiTIGR04271. ThiI_C_thiazole. 1 hit.
    TIGR00342. TIGR00342. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS51165. THUMP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3KJG6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLIVKVFPE ITIKSRPVRT KFIRQLAKNI RTVLRDLDPA VVVNGVWDNL    50
    ELETRVTDPK ALKEMGERLT CMPGIAHFLQ IDEYPLGDFD DITEKCKQHY 100
    GDALAGKIFS VRCKRAGKHA FSSMDVEKYV GSKLRRECGA AGIDLKQPEI 150
    EVRIEVRDKR LFVIHSQHNG IGGYPLGALE QTLVLMSGGF DSTVAAYQIL 200
    RRGLMTHFCF FNLGGRAHEL GVMEVAHFIW KKYGSSQRVL FVSVPFEEVL 250
    GEILGKVDDS HMGVVLKRMM LRAASSIAER LHIDALVTGE AISQVSSQTL 300
    PNLSVIDCVT DKLVLRPLIV AHKQDIIDTA DQIGTGDFAR HMPEYCGVIS 350
    VNPKTAAKRG RVEHEEQEFD MAVLERALAN ARLVPIDRVI DELGQDLQIE 400
    EVSEALAGQI VIDIRHPDAA EDDPLELAGI EVQTMPFYAV NARFKELDPT 450
    RQYLLYCDKG VMSRLHAHHL LSEGHANVRV YRPS 484
    Length:484
    Mass (Da):54,193
    Last modified:November 8, 2005 - v1
    Checksum:iABD3F5C83DDE633C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000094 Genomic DNA. Translation: ABA72090.1.
    RefSeqiWP_011332034.1. NC_007492.2.
    YP_346079.1. NC_007492.2.

    Genome annotation databases

    EnsemblBacteriaiABA72090; ABA72090; Pfl01_0346.
    GeneIDi3713374.
    KEGGipfo:Pfl01_0346.
    PATRICi19882720. VBIPseFlu44242_0347.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000094 Genomic DNA. Translation: ABA72090.1 .
    RefSeqi WP_011332034.1. NC_007492.2.
    YP_346079.1. NC_007492.2.

    3D structure databases

    ProteinModelPortali Q3KJG6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 205922.Pfl01_0346.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABA72090 ; ABA72090 ; Pfl01_0346 .
    GeneIDi 3713374.
    KEGGi pfo:Pfl01_0346.
    PATRICi 19882720. VBIPseFlu44242_0347.

    Phylogenomic databases

    eggNOGi COG0301.
    HOGENOMi HOG000227469.
    KOi K03151.
    OMAi KLFPEIM.
    OrthoDBi EOG6TBHGR.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .
    BioCyci PFLU205922:GJBD-354-MONOMER.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPi MF_00021. ThiI.
    InterProi IPR001763. Rhodanese-like_dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR026340. Thiazole_biosynth_dom.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view ]
    Pfami PF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view ]
    SMARTi SM00981. THUMP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    TIGRFAMsi TIGR04271. ThiI_C_thiazole. 1 hit.
    TIGR00342. TIGR00342. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS51165. THUMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pf0-1.

    Entry informationi

    Entry nameiTHII_PSEPF
    AccessioniPrimary (citable) accession number: Q3KJG6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: November 8, 2005
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3