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Q3KJG6

- THII_PSEPF

UniProt

Q3KJG6 - THII_PSEPF

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Protein

tRNA sulfurtransferase

Gene

thiI

Organism
Pseudomonas fluorescens (strain Pf0-1)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei267 – 2671ATPUniRule annotation
Binding sitei289 – 2891ATP; via amide nitrogenUniRule annotation
Binding sitei298 – 2981ATPUniRule annotation
Active sitei457 – 4571Cysteine persulfide intermediateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi185 – 1862ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. sulfurtransferase activity Source: UniProtKB-HAMAP
  3. tRNA adenylyltransferase activity Source: InterPro
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-HAMAP
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
  3. thiazole biosynthetic process Source: InterPro
  4. tRNA thio-modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciPFLU205922:GJBD-354-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:Pfl01_0346
OrganismiPseudomonas fluorescens (strain Pf0-1)
Taxonomic identifieri205922 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002704: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484tRNA sulfurtransferasePRO_1000074253Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi346 ↔ 457Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi205922.Pfl01_0346.

Structurei

3D structure databases

ProteinModelPortaliQ3KJG6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 167105THUMPUniRule annotationAdd
BLAST
Domaini405 – 48379RhodaneseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation
Contains 1 rhodanese domain.UniRule annotation
Contains 1 THUMP domain.UniRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiCOG0301.
HOGENOMiHOG000227469.
KOiK03151.
OMAiKLFPEIM.
OrthoDBiEOG6TBHGR.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR001763. Rhodanese-like_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR026340. Thiazole_biosynth_dom.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
TIGRFAMsiTIGR04271. ThiI_C_thiazole. 1 hit.
TIGR00342. TIGR00342. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3KJG6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLIVKVFPE ITIKSRPVRT KFIRQLAKNI RTVLRDLDPA VVVNGVWDNL
60 70 80 90 100
ELETRVTDPK ALKEMGERLT CMPGIAHFLQ IDEYPLGDFD DITEKCKQHY
110 120 130 140 150
GDALAGKIFS VRCKRAGKHA FSSMDVEKYV GSKLRRECGA AGIDLKQPEI
160 170 180 190 200
EVRIEVRDKR LFVIHSQHNG IGGYPLGALE QTLVLMSGGF DSTVAAYQIL
210 220 230 240 250
RRGLMTHFCF FNLGGRAHEL GVMEVAHFIW KKYGSSQRVL FVSVPFEEVL
260 270 280 290 300
GEILGKVDDS HMGVVLKRMM LRAASSIAER LHIDALVTGE AISQVSSQTL
310 320 330 340 350
PNLSVIDCVT DKLVLRPLIV AHKQDIIDTA DQIGTGDFAR HMPEYCGVIS
360 370 380 390 400
VNPKTAAKRG RVEHEEQEFD MAVLERALAN ARLVPIDRVI DELGQDLQIE
410 420 430 440 450
EVSEALAGQI VIDIRHPDAA EDDPLELAGI EVQTMPFYAV NARFKELDPT
460 470 480
RQYLLYCDKG VMSRLHAHHL LSEGHANVRV YRPS
Length:484
Mass (Da):54,193
Last modified:November 8, 2005 - v1
Checksum:iABD3F5C83DDE633C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000094 Genomic DNA. Translation: ABA72090.1.
RefSeqiYP_346079.1. NC_007492.2.

Genome annotation databases

EnsemblBacteriaiABA72090; ABA72090; Pfl01_0346.
GeneIDi3713374.
KEGGipfo:Pfl01_0346.
PATRICi19882720. VBIPseFlu44242_0347.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000094 Genomic DNA. Translation: ABA72090.1 .
RefSeqi YP_346079.1. NC_007492.2.

3D structure databases

ProteinModelPortali Q3KJG6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 205922.Pfl01_0346.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABA72090 ; ABA72090 ; Pfl01_0346 .
GeneIDi 3713374.
KEGGi pfo:Pfl01_0346.
PATRICi 19882720. VBIPseFlu44242_0347.

Phylogenomic databases

eggNOGi COG0301.
HOGENOMi HOG000227469.
KOi K03151.
OMAi KLFPEIM.
OrthoDBi EOG6TBHGR.

Enzyme and pathway databases

UniPathwayi UPA00060 .
BioCyci PFLU205922:GJBD-354-MONOMER.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPi MF_00021. ThiI.
InterProi IPR001763. Rhodanese-like_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR026340. Thiazole_biosynth_dom.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view ]
Pfami PF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view ]
SMARTi SM00981. THUMP. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
TIGRFAMsi TIGR04271. ThiI_C_thiazole. 1 hit.
TIGR00342. TIGR00342. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
PS51165. THUMP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pf0-1.

Entry informationi

Entry nameiTHII_PSEPF
AccessioniPrimary (citable) accession number: Q3KJG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 8, 2005
Last modified: October 29, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3