ID   HUTH_PSEPF              Reviewed;         510 AA.
AC   Q3KJE6;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   16-JUN-2009, entry version 33.
DE   RecName: Full=Histidine ammonia-lyase;
DE            Short=Histidase;
DE            EC=4.3.1.3;
GN   Name=hutH; OrderedLocusNames=Pfl01_0366;
OS   Pseudomonas fluorescens (strain Pf0-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=205922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J.,
RA   Larimer F., Land M., Kyripides N., Anderson I., Richardson P.;
RT   "Complete sequence of Pseudomonas fluorescens PfO-1.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: L-histidine = urocanate + NH(3).
CC   -!- PATHWAY: Amino-acid degradation; L-histidine degradation into L-
CC       glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC   -!- PTM: Contains an active site 4-methylidene-imidazol-5-one (MIO),
CC       which is formed autocatalytically by cyclization and dehydration
CC       of residues Ala-Ser-Gly (By similarity).
CC   -!- SIMILARITY: Belongs to the PAL/histidase family.
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DR   EMBL; CP000094; ABA72110.1; -; Genomic_DNA.
DR   RefSeq; YP_346099.1; -.
DR   SMR; Q3KJE6; 2-510.
DR   GeneID; 3713414; -.
DR   GenomeReviews; CP000094_GR; Pfl01_0366.
DR   KEGG; pfo:Pfl01_0366; -.
DR   HOGENOM; Q3KJE6; -.
DR   OMA; Q3KJE6; FAPDIEA.
DR   BioCyc; PFLU205922:PFL_0366-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016211; F:ammonia ligase activity; IEA:InterPro.
DR   GO; GO:0004397; F:histidine ammonia-lyase activity; IEA:HAMAP.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   GO; GO:0006548; P:histidine catabolic process; IEA:HAMAP.
DR   HAMAP; MF_00229; -; 1.
DR   InterPro; IPR005921; HutH.
DR   InterPro; IPR001106; Phe/His_NH3-lyase.
DR   Pfam; PF00221; PAL; 1.
DR   TIGRFAMs; TIGR01225; hutH; 1.
DR   PROSITE; PS00488; PAL_HISTIDASE; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Histidine metabolism; Lyase.
FT   CHAIN         1    510       Histidine ammonia-lyase.
FT                                /FTId=PRO_1000021566.
FT   MOD_RES     144    144       2,3-didehydroalanine (Ser) (By
FT                                similarity).
FT   CROSSLNK    143    145       5-imidazolinone (Ala-Gly) (By
FT                                similarity).
SQ   SEQUENCE   510 AA;  54108 MW;  8646F690D1E5D65A CRC64;
     MTALNLIPGQ LSLAQLRDVY QNPVKLTLDN SASAQIEASV ACVEQILAEN RTAYGINTGF
     GLLASTRIAS EDLENLQRSL VLSHAAGVGQ PISDELVRLI MVLKVNSLSR GFSGIRRVVI
     DALIALINAE VYPHIPLKGS VGASGDLAPL AHMSLVLLGE GKARHKGEWM DATEALKVAG
     LTPLTLAAKE GLALLNGTQV STAFALRGLF EGEDLFAGAL ALGGLTVEAV LGSRSPFDAR
     IHAARGQKGQ IDAAAAYRDL LGERSEVSDS HQNCEKVQDP YSLRCQPQVM GACLTQFRQA
     AEVLAIEANA VSDNPLVFAA EGDVISGGNF HAEPVAMAAD NMALAIAEIG SLSERRISLM
     MDKHMSQLPP FLVANGGVNS GFMIAQVTAA ALASENKALS HPHSVDSLPT SANQEDHVSM
     APAAGKRLWE MAENTRGILA VEWLAAVQGL DLRNGLKTSA KLEQARGILR REVPFYEKDR
     FFAPDINAAT ELLASRILTE LVPAKLLPSL
//