Reviewed, UniProtKB/Swiss-Prot Q3KJE6 (HUTH_PSEPF)

Names and origin

Protein names

Recommended name:
    Histidine ammonia-lyase
      Short name=Histidase
    EC=4.3.1.3

Gene names

Name:	hutH
Ordered Locus Names:	Pfl01_0366

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-histidine = urocanate + NH3. HAMAP MF_00229
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3. HAMAP MF_00229
Subcellular location	Cytoplasm Potential.
Post-translational modification	Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly By similarity.
Sequence similarities	Belongs to the PAL/histidase family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro histidine catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ammonia ligase activity Inferred from electronic annotation. Source: InterPro histidine ammonia-lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Histidine ammonia-lyase HAMAP MF_00229		PRO_1000021566
Amino acid modifications
	Modified residue	144	1	2,3-didehydroalanine (Ser) By similarity
	Cross-link	143 ↔ 145		5-imidazolinone (Ala-Gly) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3KJE6-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 8646F690D1E5D65A	FASTA	510	54,108
	10 20 30 40 50 60 MTALNLIPGQ LSLAQLRDVY QNPVKLTLDN SASAQIEASV ACVEQILAEN RTAYGINTGF 70 80 90 100 110 120 GLLASTRIAS EDLENLQRSL VLSHAAGVGQ PISDELVRLI MVLKVNSLSR GFSGIRRVVI 130 140 150 160 170 180 DALIALINAE VYPHIPLKGS VGASGDLAPL AHMSLVLLGE GKARHKGEWM DATEALKVAG 190 200 210 220 230 240 LTPLTLAAKE GLALLNGTQV STAFALRGLF EGEDLFAGAL ALGGLTVEAV LGSRSPFDAR 250 260 270 280 290 300 IHAARGQKGQ IDAAAAYRDL LGERSEVSDS HQNCEKVQDP YSLRCQPQVM GACLTQFRQA 310 320 330 340 350 360 AEVLAIEANA VSDNPLVFAA EGDVISGGNF HAEPVAMAAD NMALAIAEIG SLSERRISLM 370 380 390 400 410 420 MDKHMSQLPP FLVANGGVNS GFMIAQVTAA ALASENKALS HPHSVDSLPT SANQEDHVSM 430 440 450 460 470 480 APAAGKRLWE MAENTRGILA VEWLAAVQGL DLRNGLKTSA KLEQARGILR REVPFYEKDR 490 500 510 FFAPDINAAT ELLASRILTE LVPAKLLPSL

References

[1]

"Complete sequence of Pseudomonas fluorescens PfO-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyripides N., Anderson I., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000094 Genomic DNA. Translation: ABA72110.1.
RefSeq	YP_346099.1.
3D structure databases
SMR	Q3KJE6. Positions 2-510.
ModBase	Search...
Genome annotation databases
GeneID	3713414.
GenomeReviews	Gene locus Pfl01_0366 in contig CP000094_GR.
KEGG	pfo:Pfl01_0366.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3KJE6.
OMA	Q3KJE6. FAPDIEA.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_0366-MON.
Family and domain databases
HAMAP	MF_00229. [Tree]
InterPro	IPR005921. HutH. IPR001106. Phe/His_NH3-lyase. [Graphical view]
Pfam	PF00221. PAL. 1 hit. [Graphical view]
TIGRFAMs	TIGR01225. hutH. 1 hit.
PROSITE	PS00488. PAL_HISTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HUTH_PSEPF

Accession

Primary (citable) accession number: Q3KJE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)