Reviewed, UniProtKB/Swiss-Prot Q3KHV3 (HGD_PSEPF)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	Pfl01_0910

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Homogentisate 1,2-dioxygenase HAMAP MF_00334		PRO_0000225792
Sites
	Metal binding	329	1	Iron By similarity
	Metal binding	335	1	Iron By similarity
	Metal binding	365	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3KHV3-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 796796E8D13A483B	FASTA	431	47,490
	10 20 30 40 50 60 MNLDSALAYQ SGFGNEFSSE ALPGALPVGQ NSPQKAPYGL YTELFSGTAF TMTRSEARRT 70 80 90 100 110 120 WMYRIQPSAN HPAFVKLERQ LAGGPLGEVT PNRLRWNPLD LPTEPTDFID GLVSMAANSG 130 140 150 160 170 180 ADKPAGISIY HYGANRSMER VFFNADGELL LVPQLGRLRI ATELGVLDVA PLEIAVLPRG 190 200 210 220 230 240 LKFRVELLDP QARGYVAENH GAPLRLPDLG PIGSNGLANP RDFLTPVAAY ENLQQPTTLV 250 260 270 280 290 300 QKFLGQLWGT ELNHSPLNVV AWHGNNVPYK YDLRRFNTIG TVSFDHPDPS IFTVLTSPTS 310 320 330 340 350 360 VHGLANLDFV IFPPRWMVAE NTFRPPWFHR NLMNEFMGLI QGEYDAKAEG FVPGGASLHS 370 380 390 400 410 420 CMSAHGPDGE TCTKAINADL KPAKIDNTMA FMFETSQVLR PSRFALDCPQ LQSTYDACWA 430 TLPATFDPTR R

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:RESEARCH51.1-RESEARCH51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000094 Genomic DNA. Translation: ABA72653.1.
RefSeq	YP_346642.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3KHV3.
Genome annotation databases
GeneID	3715813.
GenomeReviews	Gene locus Pfl01_0910 in contig CP000094_GR.
KEGG	pfo:Pfl01_0910.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3KHV3.
OMA	HRNCMSE.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_0910-MON.
Family and domain databases
HAMAP	MF_00334. [Tree]
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_PSEPF

Accession

Primary (citable) accession number: Q3KHV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)