Reviewed, UniProtKB/Swiss-Prot Q3KHR0 (ALGL_PSEPF)

Names and origin

Protein names

Recommended name:
    Alginate lyase
    EC=4.2.2.3
Alternative name(s):
    Poly(beta-D-mannuronate) lyase
    Poly(mana) alginate lyase

Gene names

Name:	algL
Ordered Locus Names:	Pfl01_0953

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Depolymerizes alginate by cleaving the beta-1,4 glycosidic bond By similarity.
Catalytic activity	Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends. HAMAP MF_00557
Subcellular location	Periplasm Potential.
Sequence similarities	Belongs to the polysaccharide lyase 5 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alginic acid catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	poly(beta-D-mannuronate) lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 374	348	Alginate lyase HAMAP MF_00557		PRO_5000001586

Sequences

	Sequence		Length	Mass (Da)
	Q3KHR0-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: D133F033DC12D47C	FASTA	374	42,113
	10 20 30 40 50 60 MRNPKLKTLL APTLLGLAIF AGTAQAAAPL RPPQGYFAPV DKFKTGDKSE GCDAMPTPYT 70 80 90 100 110 120 GPLQFRSKYE GSDKARATLN VQSEKAFRDT TKDITTLERG TAKRVMQFMR DGRPEQLDCT 130 140 150 160 170 180 LNWLTAWAKA DALMSKDFNH TGKSMRKWAL GSMASSYIRL KFSDSHPLAQ HQQEAQVIEA 190 200 210 220 230 240 WFSKMADQVV SDWDNLPLEK TNNHSYWAAW SVMATSVATN RRDLFDWAVK EYKVGVNQVD 250 260 270 280 290 300 ADGFLPNELK RQQRALAYHN YALPPLAMIA SFAQVNGVDL RQENNGALKR LGDRVLAGVK 310 320 330 340 350 360 DPDQFEKKNG KEQDMTDLKQ DMKFAWLEPF CTLYTCSPDV IEKKHGMQPF KTFRLGGDLT 370 KVYDPSHEKG NKGS

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:RESEARCH51.1-RESEARCH51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000094 Genomic DNA. Translation: ABA72696.1.
RefSeq	YP_346685.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3KHR0.
Protein family/group databases
CAZy	PL5. Polysaccharide Lyase Family 5.
Genome annotation databases
GeneID	3715916.
GenomeReviews	Gene locus Pfl01_0953 in contig CP000094_GR.
KEGG	pfo:Pfl01_0953.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3KHR0.
OMA	RSKYEGS.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_0953-MON.
Family and domain databases
HAMAP	MF_00557. [Tree]
InterPro	IPR008397. Alginate_lyase. [Graphical view]
Gene3D	G3DSA:1.50.10.110. Alginate_lyase. 1 hit.
Pfam	PF05426. Alginate_lyase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALGL_PSEPF

Accession

Primary (citable) accession number: Q3KHR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 8, 2005
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)