Q3KHM8 (RLMF_PSEPF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	Pfl01_0985

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349930

Sequences

	Sequence		Length	Mass (Da)
	Q3KHM8 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 2DD6A809EEA527D1	FASTA	340	38,148
	10 20 30 40 50 60 MNAPRTPKPA RKKPDSATPA KPVEPRKEAS LHPRNRHQGR YDFPALIKTT PELAKFVITN 70 80 90 100 110 120 PYGKESIDFA SPDAVRVFNR ALLKSFYGIQ HWDIPADYLC PPVPGRADYI HFLADLLASN 130 140 150 160 170 180 NDGVVPRGAI VNVLDIGMGA NCVYPLIGNS EYRWHFLGSE IDPTAVAAAR AIVQSNDLNK 190 200 210 220 230 240 VIQLRQQENR KHILIGLLEP GERFDLTMCN PPFHASMEEA TKGSERKWRA LGKADPKRKL 250 260 270 280 290 300 PVLNFGGQSA ELWCEGGEAR FVTQLIAESA NFAHKVLWFS TLVSKASNLP AIETALKKAG 310 320 330 340 ALESQVVEMS QGQKQSRFVA WTFQTKSEQQ IWRRERWVRK

References

[1]

"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pf0-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000094 Genomic DNA. Translation: ABA72728.1.
RefSeq	YP_346717.1. NC_007492.2.
3D structure databases
ProteinModelPortal	Q3KHM8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3KHM8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3715968.
GenomeReviews	Gene locus Pfl01_0985 in contig CP000094_GR.
KEGG	pfo:Pfl01_0985.
PATRIC	19884053. VBIPseFlu44242_1003.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
OMA	WSFVGSD.
ProtClustDB	PRK11727.
Enzyme and pathway databases
BioCyc	PFLU205922:PFL_0985-MONOMER.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_PSEPF

Accession

Primary (citable) accession number: Q3KHM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	November 8, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program