Reviewed, UniProtKB/Swiss-Prot Q3KHM8 (RLMF_PSEPF)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase F
    EC=2.1.1.48
Alternative name(s):
    23S rRNA mA1618 methyltransferase
    rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	Pfl01_0985

Organism

Pseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]

Taxonomic identifier

205922 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine. HAMAP MF_01848
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. METT10D/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA modification Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349930

Sequences

	Sequence		Length	Mass (Da)
	Q3KHM8-1 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 2DD6A809EEA527D1	FASTA	340	38,148
	10 20 30 40 50 60 MNAPRTPKPA RKKPDSATPA KPVEPRKEAS LHPRNRHQGR YDFPALIKTT PELAKFVITN 70 80 90 100 110 120 PYGKESIDFA SPDAVRVFNR ALLKSFYGIQ HWDIPADYLC PPVPGRADYI HFLADLLASN 130 140 150 160 170 180 NDGVVPRGAI VNVLDIGMGA NCVYPLIGNS EYRWHFLGSE IDPTAVAAAR AIVQSNDLNK 190 200 210 220 230 240 VIQLRQQENR KHILIGLLEP GERFDLTMCN PPFHASMEEA TKGSERKWRA LGKADPKRKL 250 260 270 280 290 300 PVLNFGGQSA ELWCEGGEAR FVTQLIAESA NFAHKVLWFS TLVSKASNLP AIETALKKAG 310 320 330 340 ALESQVVEMS QGQKQSRFVA WTFQTKSEQQ IWRRERWVRK

References

[1]

"Complete sequence of Pseudomonas fluorescens PfO-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Saunders E.H., Schmutz J., Larimer F., Land M., Kyripides N., Anderson I., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000094 Genomic DNA. Translation: ABA72728.1.
RefSeq	YP_346717.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3715968.
GenomeReviews	Gene locus Pfl01_0985 in contig CP000094_GR.
KEGG	pfo:Pfl01_0985.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3KHM8.
OMA	Q3KHM8. LNFGGQN.
Family and domain databases
HAMAP	MF_01848. [Tree]
InterPro	IPR016909. S-AdoMet-dep_MeTrfase_YbiN_prd. IPR010286. SAM-Mtase_prd. [Graphical view]
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_PSEPF

Accession

Primary (citable) accession number: Q3KHM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)