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Q3KHL4 (PUR4_PSEPF) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3
Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Gene names
Name:purL
Ordered Locus Names:Pfl01_0999
OrganismPseudomonas fluorescens (strain Pf0-1) [Complete proteome] [HAMAP]
Taxonomic identifier205922 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length1298 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12981298Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000264588

Regions

Domain1045 – 1298254Glutamine amidotransferase type-1
Nucleotide binding305 – 31612ATP Potential

Sites

Active site11381Nucleophile By similarity
Active site12631 By similarity
Active site12651 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3KHL4 [UniParc].

Last modified November 8, 2005. Version 1.
Checksum: 85DD6C14A2A2D090

FASTA1,298140,904
        10         20         30         40         50         60 
MLILRGAPAL SAFRHSKLLE QLSQKVPAVS GLYAEFAHFA EVTGVLTGDE QQVLARLLKY 

        70         80         90        100        110        120 
GPSVPVQEPT GRLFLVLPRF GTISPWSSKA SDIARNCGLS KIQRLERGIA FYVAGQFSET 

       130        140        150        160        170        180 
EAQQIADVLH DRMTQIVLAN LEQAAGLFSH AEPKPLTAID ILGGGRAALE KANVELGLAL 

       190        200        210        220        230        240 
AEDEIDYLVN AFNGLKRNPH DIELMMFAQA NSEHCRHKIF NASWDIDGES QEKSLFGMIK 

       250        260        270        280        290        300 
NTYQMHSEGV LSAYKDNASV IVGNVAGRFF PDPETRQYGA VQEPVHILMK VETHNHPTAI 

       310        320        330        340        350        360 
APFPGASTGS GGEIRDEGAT GRGAKPKAGL TGFTVSNLQI PGFEQPWEVP YGKPERIVNA 

       370        380        390        400        410        420 
LDIMIEGPLG GAAFNNEFGR PALTGYFRTF EQSITTPHGD EVRGYHKPIM LAGGMGNIRE 

       430        440        450        460        470        480 
EHVKKGEIVV GSKLVVLGGP AMLIGLGGGA ASSMATGTSS ADLDFASVQR ENPEMERRCQ 

       490        500        510        520        530        540 
EVIDRCWQLG DKNPISFIHD VGAGGLSNAF PELVNDGDRG GRFELRNIPN DEPGMAPHEI 

       550        560        570        580        590        600 
WSNESQERYV LAVGPADFER FKAICERERC PFAVVGEATA EPQLTVTDSH FGNNPVDMPL 

       610        620        630        640        650        660 
EVLLGKAPRM HRSVVREAEL GDDFDPSNLD IGESIERVLH HPAVASKSFL ITIGDRTITG 

       670        680        690        700        710        720 
LVARDQMVGP WQVPVADVAV TATSFDVYTG EAMAMGERTP LALLDAPASG RMAIGETITN 

       730        740        750        760        770        780 
IAASRINKLS DIKLSANWMS AAGHPGEDAR LYDTVKAVGM ELCPELGITI PVGKDSMSMA 

       790        800        810        820        830        840 
TRWNDNGEDK TVTSPMSLIV TGFAPVADIR QTLTPELRMD KGTTDLILID LGRGQNRMGA 

       850        860        870        880        890        900 
SILAQVHGKL GKQAPDVDDA EDLKAFFAVI QGLNADGHLL AYHDRSDGGL LTSVMEMAFA 

       910        920        930        940        950        960 
GHCGLSLNLD SVAESSAEIA AILFNEELGA VIQVRQDATP DILAQFSAAG LGDCVSVIGQ 

       970        980        990       1000       1010       1020 
PINNGQINIT FNGDTVFEGQ RRLLQRQWAE TSYQIQRLRD NADCAEQEFD ALLEEDNPGL 

      1030       1040       1050       1060       1070       1080 
SVKLSYDVNQ DIAAPYIKKG IRPQVAVLRE QGVNGQVEMA AAFDRAGFNA IDVHMSDILA 

      1090       1100       1110       1120       1130       1140 
GRVDLNEFKG LVACGGFSYG DVLGAGEGWA KSALFNSRAR DAFQGFFERN DSFTLGVCNG 

      1150       1160       1170       1180       1190       1200 
CQMMSNLHEL IPGSEFWPHF VRNRSEQFEA RVAMVQVQES NSIFLQGMAG SRMPIAIAHG 

      1210       1220       1230       1240       1250       1260 
EGHAEFESEE ALLEADLSGC VAMRFVDNHG KVTEAYPANP NGSPRGITGL TSRDGRVTIM 

      1270       1280       1290 
MPHPERVFRA VQNSWRSEDW NEDAPWMRMF RNARVWVN

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000094 Genomic DNA. Translation: ABA72742.1.
RefSeqYP_346731.1. NC_007492.2.

3D structure databases

HSSPHSSP built from PDB template 1VK3 based on UniProtKB Q9X0X3.
ProteinModelPortalQ3KHL4.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ3KHL4.

PTM databases

PhosSiteQ3KHL4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3716001.
GenomeReviewsGene locus Pfl01_0999 in contig CP000094_GR.
KEGGpfo:Pfl01_0999.
PATRIC19884081. VBIPseFlu44242_1017.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0046.
HOGENOMHBG335309.
OMAVKAVGME.
PhylomeDBQ3KHL4.
ProtClustDBPRK05297.

Enzyme and pathway databases

BioCycPFLU205922:PFL_0999-MONOMER.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR4_PSEPF
AccessionPrimary (citable) accession number: Q3KHL4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 8, 2005
Last modified: January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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